LabChip® EZ Reader Substrate Selection Guide Substrate name Cat. No. Sequence Recommended Enzymes ProfilerPro Peptide 1 760345 5-FAM-AKRRRLSSLRA-COOH IKKα, IKKβ, IKKe, LOK, PAK2, PAK3, PKG1α, PKG1β, ROCK2, ROCK1, DAPK1, ZIPK, PIM3, CLK3 ProfilerPro Peptide 2 760346 5-FAM-EAIYAAPFAKKK-CONH2 Abl, Abl(Q252H), Abl(T315I), Abl(H396P), Abl(E255K), Abl(Y253F),Abl(G250E), FGR, Arg, BTK, Flt-3, Flt-3(D835Y), MET, KIT, RET ProfilerPro Peptide 4 760348 5-FAM-EGIYGVLFKKK-CONH2 FYN, LCK, LYN, SRC, Hck, BLK ProfilerPro Peptide 5 760349 5-FAM-GQLIDSMANSFVGTR-CONH2 cRAF ProfilerPro Peptide 6 760350 5-FAM-GRPRTSSFAEG-CONH2 AKT1, AKT2, AKT3, MSK1, MSK2, SGK1 ProfilerPro Peptide 7 760351 5-FAM-HMRSAMSGLHLVKRR-COOH AMPKα1 ProfilerPro Peptide 8 760352 5-FAM-IPTSPITTTYFFFKKK-COOH Erk1, Erk2, CDK6, IRAK4, p38α,p38a(T106M), p38β2, p38δ, p38γ ProfilerPro Peptide 10 760354 5-FAM-KKKVSRSGLYRSPSMPENLNRPR-COOH AMPKa2/b1/1, BRSK1, BRSK2, CHK1, CHK2, MARK1, MARK2, MARK3, MARK4,MNK1, NuaK1, c-TAK1, TSSK1, TSSK2 ProfilerPro Peptide 11 760355 5-FAM-KKLNRTLSVA-COOH CAMK2α, CAMK2β, CAMK2γ, CAMK4, RSK1, RSK2, RSK3, MAPKAPK3, NEK2, SGK2, SGK3, PhKg1,PhKg2, DCAML1, PKN1, PKN2, PASK ProfilerPro Peptide 12 760356 5-FAM-KKLRRTLSVA-COOH Camk1a, Camk1d, MAPKAPK2, PKD1, PKD2, PDK3, MAPKAPK5 (PRAK), MELK ProfilerPro Peptide 13 760357 5-FAM-KKSRGDYMTMQIG-CONH2 DDR2, INSR, IGF1R, MST2, FGFR1, ALK 1 Substrate name Cat. No. Sequence Recommended Enzymes ProfilerPro Peptide 14 760358 5-FAM-KRELVEPLTPSGEAPNQALLR-CONH2 JNK2 ProfilerPro Peptide 15 760359 5-FAM-KRREILSRRPpSYR-COOH GSK3β, GSK3a ProfilerPro Peptide 16 760360 5-FAM-KRRRALpSVASLPGL-CONH2 CK1δ, CK1ε, CSNK1a, CK1g1, CK1g2, CK1g3 ProfilerPro Peptide 18 760362 5-FAM-QSPKKG-CONH2 CDK2 ProfilerPro Peptide 19 760363 5-FAM-RFARKGSLRQKNV-COOH PKCα, PKCβ1, PKCβ2, PKCγ, PKCδ, PKCε, PKCξ PKCΘ, PKCι ProfilerPro Peptide 20 760364 5-FAM-RSRHSSYPAGT-CONH2 PIM1, PIM2 ProfilerPro Peptide 21 760365 5-FAM-LRRASLG-CONH2 PKACα, AurA, AurB, AurC, PRKX ProfilerPro Peptide 22 760366 5-FAM-EEPLYWSFPAKKK-CONH2 KDR (VEGFR2), EGFR, EGFR(T790M), EGFR( T790M,L856R), FER, BMX, FGFR1(V561M), FGFR2, FGFR2(N549H), FGFR3, FGFR3(K650E), FGFR4, FRK, HER4, JAK2, LTK, ROS, RET, RET(Y791F), RET(V804L), SYK,TEC, YES ProfilerPro Peptide 24 760387 5-FAM-KKISGRLSPIMTEQ-CONH2 DYRK1α, DYRK1b, DYRK3, DYRK4, HIPK1, HIPK2, HIPK3 ProfilerPro Peptide 25 760388 5-FAM-VDGKEIYNTIRRK-CONH2 HGK, GCK, MINK1, MST1, MST3, NEK1, CLK2 ProfilerPro Peptide 26 760389 5-FAM-ARKRERTYSFGHHA-COOH P70S6K ProfilerPro Peptide 27 760424 5-FAM-EFPIYDFLPAKKK-CONH2 FAK2, EphA1,EphA2, EphA3, EphA4,EphA5, EpHa8, EpHB1, EphB2,EphB3, EphB4, FGFR3, LynB, FES, PYK2, TXK, ITK, TYRO3, TRKB, TRKC, MER, SRM ProfilerPro Peptide 29 760429 5-FAM-GGGPATPKKAKKL-CONH2 CDK2/-25, CDC2/cyclinB1, CDK3 2 Substrate name Cat. No. Sequence Recommended Enzymes ProfilerPro Peptide 30 760430 5-FAM-KKKKEEIYFFF-CONH2 FLT1, FLT4, FMS, AXL, PDGFRa, PDGFRa(D842V), PDGFRb, MSTR, KIT, KIT(T670I), MET(M1250T) ProfilerPro Peptide 31 760480 5-FAM-RRRLSFAEPG-CONH2 PAK4, PAK5(PAK7) ProfilerPro Peptide 32 760641 5-FAM-FLAKSFGSPNRAYKK-CONH2 NEK6 NEK7 NEK9 ProfilerPro Peptide 33 760642 5-FAM-C6-RLGRDKYKTLRQIRQ-CONH2 LRRK2 TAOK3 YSK SLK ProfilerPro Peptide 34 760643 5-FAM-RRRFRPASPLRGPPK-COOH DYRK2 MSSK1 CDK1 CDK4 CDK9 HIPK3 HIPK4 CLK1 CLK3 SRPK2 iFL-cAMP 760444 Proprietary Phosphodiesterase Assays iFL-cAMP 760445 Proprietary Phosphodiesterase Assays Histone 3 Acetylated Peptide 760511 FITC-TGGK(Ac)APR-COOH Epigenetic Assays p53 Acetylated Peptide 760512 FITC-TSPQPKK(Ac)-CONH2 Epigenetic Assays Histone 4 Acetylated Peptide 760513 FITC-LGKGGAK(Ac)-CONH2 Epigenetic Assays PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2012, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. Version created 26 June 2012. 3