TECHNICAL DATA SHEET 32 P Caution: For Laboratory Use. A product for research purposes only. Cytidine 3’,5’-bis(phosphate), [5’ -32P]Product Number: BLU019A NH 2 LOT SPECIFIC INFORMATION N Lot Number: ____07071____ O Specific Activity: ____3000 ___ Ci/mmol N HO ___ 111_____ TBq/mmol HO O 32P Concentration: ____10.0_____ mCi/ml O O ____370.0_____MBq/ml HO O HO ____3.33_____ μM Calibration Date: ___16-July-2011___ OH P O M.W. 405.18 C 9H15N 3O1132P2 PACKAGING: 10.0mCi/ml (370.0 MBq/ml) on the Calibration Date. 10mM Tricine pH 7.6 solution and shipped in a plastic container on dry ice. The lead free container is non toxic and environmentally friendly. STABILITY AND STORAGE: Cytidine 3’,5’-bis(phosphate), [5’ -32P]-should be stored at -20oC in its original solvent and at its original concentration. Lot to lot variation may occur, and it is advisable to check purity prior to use. This product will undergo decomposition if left at room temperature for long periods. It is recommended that the product remain on ice while in use. The product can be briefly thawed at room temperature or quick-thawed in a 37oC water bath. Multiple thawing and freezing will not affect product purity if care is taken to minimize the time spent at room temperature. Premixing and aliquoting the product is recommended if feasible. HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause cancer. QUALITY CONTROL: Radiochemical Purity: This lot was initially found to be >95% when determined by the following analytical HPLC method using a C-18 column. Elution is isocratic by a mixture of 70% Solvent A (20mM potassium phosphate, 20mM phosphoric acid containing 10mM tetrabutylammonium hydroxide) and Solvent B (30% methanol). The radiochemical purity is usually greater than 99% when purified. Isotopic Purity: All lots of 32P are typically > 99.9% pure. Page 1 of 2 PREPARATIVE PROCEDURE: Each lot has been purified by HPLC using an anion exchange resin eluting with Triethylammoniumbicarbonate. The purified nucleotide will be in the triethylammonium salt form. Consistently high quality Synthesized diluted and packaged using state of the art automation. SAFE HANDLING: Film badges should be used by all personnel working with 32P. If millicurie quantities are manipulated, finger badges are recommended. Acrylic plastic (1/4 inch) is the recommended shielding for working with quantities of 32P up to 10mCi. A radiation protection specialist should be consulted for specific applications. Whenever working with 32P on the open bench, the eyes should be shielded with approved safety glasses. DISPOSAL: Hold for decay; specific regulations should be addressed with your radiation safety officer. SPECIAL INFORMATION: Visit www.perkinelmer.com/toolkit to use our online Radioactive Decay Calculator. Specific Activity Before Calibration Date The specific activity is specified as of the calibration date. This must be taken into consideration when calculating concentration in mass-dependent applications. The specific activity on any day prior to the calibration date can be calculated using the formula: Specific Activity After Calibration Date The specific activity on any day after the calibration date can be calculated using the formula: Where: SA = Specific Activity expressed as Ci/mmol SA cal = Specific Activity on the calibration date. DF = Fraction of current radioactivity that will remain on the calibration date (from the decay chart) For example, for a date 8 days prior to the calibration date DF = 0.678. SA Theo = 9120 Ci/mmol for the theoretical specific activity of carrier free 32P. PHOSPHORUS-32 DECAY TABLE HALF LIFE= 14.3 DAYS days 0 10 20 30 40 50 60 0 1.000 0.616 0.379 0.233 0.144 0.088 0.054 1 0.953 0.587 0.361 0.222 0.137 0.084 0.052 2 0.906 0.559 0.344 0.212 0.130 0.080 0.049 3 0.865 0.532 0.328 0.202 0.124 0.077 0.047 4 0.824 0.507 0.312 0.192 0.118 0.073 0.045 5 0.785 0.483 0.297 0.183 0.113 0.069 0.043 6 0.748 0.460 0.283 0.174 0.107 0.066 0.041 7 0.712 0.436 0.270 0.166 0.102 0.063 0.039 8 0.678 0.418 0.257 0.158 0.096 0.060 0.037 9 0.646 0.396 0.245 0.151 0.093 0.057 0.035 To use the decay table above, find the number of days in the top and left hand columns of the chart, then find the corresponding decay factor. To obtain a precalibration number, divide by the decay factor. For a postcalibration number, multiply by the decay factor. PerkinElmer, Inc. 549 Albany Street Boston, MA 02118 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com/nenradiochemicals For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.