Adenosine 5`-Triphosphate, [γ-33 P]-

TECHNICAL
DATA
SHEET
33
P
Caution: For Laboratory Use. A product for research purposes only.
Adenosine 5’-Triphosphate, [γ -33P]Product Number: NEG302H
LOT SPECIFIC INFORMATION
NH2
Lot Number:
____11284____
N
N
Specific Activity: ____3000____ Ci/mmol
HO
Concentration:
____10______ mCi/ml
N
N
___ 111_____ TBq/mmol
HO
HO
O
HO
O
O
33P
P
P
O
O
O
O
____370_____ MBq/ml
____3.33_____ μM
Calibration Date: ___13-Dec-2014___
M.W. 509.18
C10H 16N5O 13P233P
OH
OH
PACKAGING: 10mCi/ml (370 MBq/ml) on the Calibration Date. 10mM Tricine pH 7.6 solution The product is shipped
on dry ice in a plastic container. The lead free container is non toxic and environmentally friendly.
STABILITY AND STORAGE: Adenosine 5’-Triphosphate, [γ -33P]- should be stored at -20oC or below in its
original solvent and at its original concentration. Lot to lot variation may occur and it is advisable to check purity prior to
use. This product will undergo decomposition if left at room temperature for long periods. It is recommended that the
product remain on ice while in use. The product can be thawed at room temperature or quick-thawed in a 37oC water bath.
Multiple thawing and freezing will not affect product purity if care is taken to minimize the time spent at room temperature.
Pre-mixing and aliquoting the product is recommended if feasible.
HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause
cancer.
QUALITY CONTROL:
Radiochemical Purity: This lot was initially found to be >99% when purified and >95% on day of shipment when
determined by the following analytical HPLC method using a C-18 column. Elution is isocratic by a mixture of 70% Solvent
A (20mM potassium phosphate, 20mM phosphoric acid containing 10mM tetrabutylammonium hydroxide) and Solvent B
(30% methanol).
Isotopic Purity: All lots of 33P are typically > 99.5% isotopically pure and may contain <0.5% 32P.
Biological Testing: Polynucleotide Kinase Assay.
Page 1 of 2
PREPARATIVE PROCEDURE: Each lot has been purified by HPLC using an anion exchange resin eluting with
triethylammoniumbicarbonate. The purified nucleotide will be in the triethylammonium salt form. Consistently high quality –
Synthesized, purified, diluted and packaged using state of the art automation.
SAFE HANDLING: At the sub-millicurie amounts used in most applications, 33P can be manipulated without any special
shielding or badge dosimetry. A radiation protection specialist should be consulted for specific applications. Whenever working
with 33P on the open bench, the eyes should be shielded with approved safety glasses.
DISPOSAL: Hold for decay; specific regulations should be addressed with your radiation safety officer.
SPECIAL INFORMATION: Visit www.perkinelmer.com/toolkit to use our online Radioactive Decay Calculator.
Specific Activity Before Calibration Date
The specific activity is specified as of the calibration date. This must be taken into consideration when calculating
concentration in mass-dependent applications. The specific activity on any day prior to the calibration date can be calculated
using the formula:
Specific Activity After Calibration Date
The specific activity on any day after the calibration date can be calculated using the formula:
Where:
SA = Specific Activity expressed as Ci/mmol
SA cal = Specific Activity on the calibration date.
DF = Fraction of current radioactivity that will remain on the calibration date (from the decay chart)
For example, for a date 8 days prior to the calibration date D F = 0.804.
SA Theo = 5200 Ci/mmol for the theoretical specific activity of carrier free 33P.
PHOSPHORUS-33 DECAY TABLE HALF LIFE= 25.4 DAYS
days
0
10
20
30
40
50
60
0
1.000
0.761
0.579
0.441
0.336
0.256
0.195
1
0.973
0.741
0.564
0.429
0.327
0.249
0.189
2
0.947
0.721
0.549
0.418
0.318
0.242
0.184
3
0.921
0.701
0.534
0.406
0.309
0.236
0.179
4
0.897
0.683
0.520
0.395
0.301
0.229
0.174
5
0.872
0.664
0.506
0.385
0.293
0.223
0.170
6
0.849
0.646
0.492
0.374
0.285
0.217
0.165
7
0.826
0.629
0.479
0.364
0.277
0.211
0.161
8
0.804
0.612
0.466
0.355
0.270
0.205
0.156
9
0.782
0.595
0.453
0.345
0.263
0.200
0.152
To use the decay table above, find the number of days in the top and left hand columns of the chart, then find the
corresponding decay factor. To obtain a precalibration number, divide by the decay factor. For a postcalibration number,
multiply by the decay factor.
PerkinElmer, Inc.
549 Albany Street
Boston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc.
All other trademarks are the property of their respective owners.