BRD4 Bromodomain 2 Catalog No. Type 15-0013 Bromodomain Mol. Wgt. 42 kDa Expressed In E. coli Epitope Tag GST Product Description: Recombinant human BRD4 Bromodomain 2 (HUNK1, accession number O60885, amino acids 341 to 470) containing an N-terminal GST tag, expressed in E. coli. BRD4 is a member of the BET sub-family of bromodomaincontaining proteins, which also includes BRD2, BRD3 and BRDT. BET proteins have two bromodomains and an ET domain C-terminal to the second bromodomain. BRD4 serves as a cofactor for Myc and is crucial to Myc’s role in acute myeloid leukemia. Inhibition of BRD4 binding suppresses Myc activity and leads to differentiation of AML cells and elimination of AML stem cells. Protein Gel Data: BRD4 Bromodomain 2 (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Formulation: Recombinant GST-tagged protein at 1 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol. Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. Application Notes: Recombinant BRD4 Bromodomain 2 is useful for protein binding and screening experiments examining acetylated protein substrates. References: This product is for in vitro research use only and is not intended for use in humans or animals. EpiCypher, Inc • Phone: 855-374-2461 • Fax: 855-420-6111 • www.epicypher.com Histone H4 K5Ac, K8Ac, K12Ac Peptide, Biotinylated Catalog No. 12-0047 Product Description: A synthetic peptide derived from human histone H4, amino acids 1-23, acetylated at lysine 5, lysine 8 and lysine 12. The alpha-amino group on the N-terminus is acetylated, with a C-terminal PEG spacer containing a biotin moiety. Formulation: 50 µg of lyophilized powder. Sequence: Ac-SGRGK(Ac)GGK(Ac)GLGK(Ac)GGAKRHRKVLR-Peg-Biot Molecular Weight: 2958 Da Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze histone H4 K5Ac, K8Ac, K12Ac peptide for identity and homogeneity. Purity: >95% as judged by HPLC and mass spectrometry. Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing. References: Fuchs SM et al (2011). Curr Biol 21: 53-58. HPLC Data: Reversed phase analytical HPLC chromatogram of purified histone H4 K5Ac, K8Ac, K12Ac peptide. This product is for in vitro research use only and is not intended for use in humans or animals.