BRD4 BD2 Technical Data Sheet

BRD4 Bromodomain 2
Catalog No.
Type
15-0013
Bromodomain
Mol. Wgt. 42 kDa
Expressed In E. coli
Epitope Tag
GST
Product Description:
Recombinant human BRD4 Bromodomain 2 (HUNK1,
accession number O60885, amino acids 341 to 470)
containing an N-terminal GST tag, expressed in E. coli.
BRD4 is a member of the BET sub-family of bromodomaincontaining proteins, which also includes BRD2, BRD3 and
BRDT. BET proteins have two bromodomains and an ET
domain C-terminal to the second bromodomain. BRD4
serves as a cofactor for Myc and is crucial to Myc’s role in
acute myeloid leukemia. Inhibition of BRD4 binding
suppresses Myc activity and leads to differentiation of AML
cells and elimination of AML stem cells.
Protein Gel Data: BRD4 Bromodomain 2 (1 µg) was
run on a PAGE gel and stained with Coomassie blue.
The migration and molecular weight of the protein
standards are indicated.
Formulation:
Recombinant GST-tagged protein at 1 mg/ml in 20 mM
Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability:
Stable for six months at -80°C from date of receipt. For
best results, aliquot and avoid multiple freeze/thaws.
Application Notes:
Recombinant BRD4 Bromodomain 2 is useful for protein
binding and screening experiments examining acetylated
protein substrates.
References:
This product is for in vitro research use only and is not intended for use in humans or animals.
EpiCypher, Inc • Phone: 855-374-2461 • Fax: 855-420-6111 • www.epicypher.com
Histone H4 K5Ac, K8Ac, K12Ac Peptide, Biotinylated
Catalog No. 12-0047
Product Description:
A synthetic peptide derived from human histone H4,
amino acids 1-23, acetylated at lysine 5, lysine 8 and lysine
12. The alpha-amino group on the N-terminus is
acetylated, with a C-terminal PEG spacer containing a
biotin moiety.
Formulation:
50 µg of lyophilized powder.
Sequence:
Ac-SGRGK(Ac)GGK(Ac)GLGK(Ac)GGAKRHRKVLR-Peg-Biot
Molecular Weight:
2958 Da
Mass Spec Data: MALDI-TOF mass spectrometry was
used to analyze histone H4 K5Ac, K8Ac, K12Ac
peptide for identity and homogeneity.
Purity:
>95% as judged by HPLC and mass spectrometry.
Storage and Stability:
Lyophilized peptides are stable for 2 years at -20°C from
date of shipment. Peptides in solution are stable for 6
months at -80°C. Spin vial briefly in microfuge and
reconstitute with sterile distilled water and vortex briefly
until resuspended. Aliquot peptide to avoid repeated
freezing and thawing.
References:
Fuchs SM et al (2011). Curr Biol 21: 53-58.
HPLC Data: Reversed phase analytical HPLC
chromatogram of purified histone H4 K5Ac, K8Ac,
K12Ac peptide.
This product is for in vitro research use only and is not intended for use in humans or animals.
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