Deepwell LumaPlate™-96: 96-Well, White, Scintillator Coated (Product Information Bulletin No. 19)

Research
Clinical Screening
Microplates
Deepwell LumaPlate TM-96
96-Well, White, Scintillator coated
ISOTOPIC
FLUORESCENCE
COLORIMETRIC
LUMINESCENCE
Description
Polystyrene
No
No
No
Not applicable
Optional
Not applicable
Typical Applications
The Deepwell LumaPlate-96 is a solid scintillator coated, 96-well microplate that eliminates the need for
adding LSC cocktail. This deepwell version has a
sample working volume of 300 µL. For analysis of
non-volatile samples, simply dispense samples into
the standard Deepwell LumaPlate wells, dry down
and count on a microplate scintillation counter, such
as the Perkin-Elmer TopCount ® or MicroBeta ®.
The LumaPlate's solid scintillator simplifies single
and dual label CPM/DPM counting by eliminating
variable chemical quench and in addition dramatically
reduces total cost per sample. Deepwell LumaPlates
are particularly suitable for samples produced from
51
Cr release assays, enzyme inhibition assays, organic
extractions, chromatography fractions, and capillary
electrophoresis fractions. Bar coded microplates are
also available.
❒ HPLC fraction analysis
❒ Larger sample volumes (up to 300 µL)
❒ Enzyme inhibition assay
❒ 51Cr release assay
Recommended Companion Products
Microplate sealing:
TM
● TopSeal -A
6005185
● TopSeal-S
6005161
TM
● MicroMate
heat sealing system (for TopSeal-S)
Instrumentation:
● TopCount Microplate Scintillation and
Luminescence Counter
● MicroBeta Microplate Scintillation and
Luminescence Counter
Features & Benefits
❒
❒
❒
❒
Sample volumes of up to 300 µL
Very low backgrounds for greater sensitivity
High counting efficiency for greater sensitivity
No variable chemical quench for maximum
signal output
❒ Easy to use with reproducible results
❒ No liquid radioactive waste - reduces disposal
costs
Ordering Information
Deepwell LumaPlate-96, 96-well, White,
Scintillator Coated
Case of 50
6005630
Bar code labeled Deepwell LumaPlates are available.
Contact your local PerkinElmer representative for details.
www.perkinelmer.com/lifesciences
No - 19
Material .............................................
Sterile ................................................
Lids ....................................................
Tissue culture treated .......................
Filter type .........................................
Bar code .............................................
White writing area ............................
PRODUCT INFORMATION BULLETIN
Drug Discovery
10.8 ± 0.1
7.15 ± 0.05
Material:
Polystyrene with white colorant.
Scintillator layer on well bottom.
( ± 0.25 mm)
( ± 0.20 mm)
( ± 0.13 mm)
14.38 ± 0.08
1
21.8 ± 0.2
2
3
4
5
6
7
8
9
10
11
12
A
B
85.47 ± 0.20
C
D
E
9.00 ± 0.08
127.76 mm
85.47 mm
14.60 mm
7.15 mm
10.80 mm
400 µL
11.24 mm
14.38 mm
11.24 ± 0.08
Dimensions (Tolerances):
Length
Width
Height
Well diameter
Well depth
Well volume (nominal)
A1 offset to top
A1 offset to side
Volume per well 400 µL
6.78 ± 0.1
( ± 0.08 mm)
( ± 0.08 mm)
F
G
H
14.6 ± 0.13
Experimental Results
6.65 ± 0.05
9.00 ± 0.08
The chromatogram below (Figure 3) shows the result of
127.76
two HPLC runs of the same rat urine. Curve B represents
a 100 µL injection with 61,500 DPM of 3 H labeled drug
and its metabolites measured on-line using the PerkinElmer Radiomatic TM Model 505TR flow scintillation analyzer. Curve A represents a 7 µL injection with only
4,300 DPM of total radioactivity measured off-line in a Deepwell LumaPlate-96 with the TopCount system. For better
visualization, the upper curve of A was also expanded by a factor of five. When comparing the classical on-line and
the new off-line approaches, it becomes clear that curve A shows much more detail than does curve B. In addition
to using a 14-fold smaller injection volume, the TopCount/Deepwell LumaPlate-96 combination provides better
sensitivity because of a much lower background, while maintaining very high counting efficiency. On a blank
Deepwell LumaPlate-96, the background was determined to be only 0-2 CPM. Every count above 4 CPM can be
interpreted as a valid tritium signal. It should also be noted that all peak intensities in both curves (A and B)
compare well to each other. The main metabolite at a retention time of 16.5 minutes is clearly visible in both curves
(A and B). This peak measured by the TopCount shows a better chromatographic resolution than the on-line
chromatogram.
PRODUCT INFORMATION BULLETIN
Technical Information
± 0.25
No - 19
Figure 3: Radioactivity chromatogram of rat urine
containing 3H labeled drug;
A = 4300 DPM (7 µL) injected and counted with
the TopCount system (8 minutes per well).
B = 61500 DPM (100 µL) injected and monitored
on-line with adding 800 µL LSC-cocktail to a
100 µL mixing cell.
Reference: Packard Application Note AN004-TC
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European Headquarters: PerkinElmer Life Sciences, Imperiastraat 8, BE-1930 Zaventem Belgium
Technical Support: in Europe: [email protected] in US and Rest of World: [email protected]
Belgium: Tel: 0800 94 540 • France: Tel: 0800 90 77 62 • Netherlands: Tel: 0800 02 23 042 • Germany: Tel: 0800 1 81 00 32 • United Kingdom: Tel: 0800 89 60 46
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LumaPlate, TopSeal, MicroMate and Radiomatic are trademarks and TopCount and MicroBeta are registered trademarks of PerkinElmer Life Sciences.
www.perkinelmer.com/lifesciences
PIB#19 - 1002 - Rev. B
© 2002 PerkinElmer Life Sciences
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