Research Clinical Screening Microplates Deepwell LumaPlate TM-96 96-Well, White, Scintillator coated ISOTOPIC FLUORESCENCE COLORIMETRIC LUMINESCENCE Description Polystyrene No No No Not applicable Optional Not applicable Typical Applications The Deepwell LumaPlate-96 is a solid scintillator coated, 96-well microplate that eliminates the need for adding LSC cocktail. This deepwell version has a sample working volume of 300 µL. For analysis of non-volatile samples, simply dispense samples into the standard Deepwell LumaPlate wells, dry down and count on a microplate scintillation counter, such as the Perkin-Elmer TopCount ® or MicroBeta ®. The LumaPlate's solid scintillator simplifies single and dual label CPM/DPM counting by eliminating variable chemical quench and in addition dramatically reduces total cost per sample. Deepwell LumaPlates are particularly suitable for samples produced from 51 Cr release assays, enzyme inhibition assays, organic extractions, chromatography fractions, and capillary electrophoresis fractions. Bar coded microplates are also available. ❒ HPLC fraction analysis ❒ Larger sample volumes (up to 300 µL) ❒ Enzyme inhibition assay ❒ 51Cr release assay Recommended Companion Products Microplate sealing: TM ● TopSeal -A 6005185 ● TopSeal-S 6005161 TM ● MicroMate heat sealing system (for TopSeal-S) Instrumentation: ● TopCount Microplate Scintillation and Luminescence Counter ● MicroBeta Microplate Scintillation and Luminescence Counter Features & Benefits ❒ ❒ ❒ ❒ Sample volumes of up to 300 µL Very low backgrounds for greater sensitivity High counting efficiency for greater sensitivity No variable chemical quench for maximum signal output ❒ Easy to use with reproducible results ❒ No liquid radioactive waste - reduces disposal costs Ordering Information Deepwell LumaPlate-96, 96-well, White, Scintillator Coated Case of 50 6005630 Bar code labeled Deepwell LumaPlates are available. Contact your local PerkinElmer representative for details. www.perkinelmer.com/lifesciences No - 19 Material ............................................. Sterile ................................................ Lids .................................................... Tissue culture treated ....................... Filter type ......................................... Bar code ............................................. White writing area ............................ PRODUCT INFORMATION BULLETIN Drug Discovery 10.8 ± 0.1 7.15 ± 0.05 Material: Polystyrene with white colorant. Scintillator layer on well bottom. ( ± 0.25 mm) ( ± 0.20 mm) ( ± 0.13 mm) 14.38 ± 0.08 1 21.8 ± 0.2 2 3 4 5 6 7 8 9 10 11 12 A B 85.47 ± 0.20 C D E 9.00 ± 0.08 127.76 mm 85.47 mm 14.60 mm 7.15 mm 10.80 mm 400 µL 11.24 mm 14.38 mm 11.24 ± 0.08 Dimensions (Tolerances): Length Width Height Well diameter Well depth Well volume (nominal) A1 offset to top A1 offset to side Volume per well 400 µL 6.78 ± 0.1 ( ± 0.08 mm) ( ± 0.08 mm) F G H 14.6 ± 0.13 Experimental Results 6.65 ± 0.05 9.00 ± 0.08 The chromatogram below (Figure 3) shows the result of 127.76 two HPLC runs of the same rat urine. Curve B represents a 100 µL injection with 61,500 DPM of 3 H labeled drug and its metabolites measured on-line using the PerkinElmer Radiomatic TM Model 505TR flow scintillation analyzer. Curve A represents a 7 µL injection with only 4,300 DPM of total radioactivity measured off-line in a Deepwell LumaPlate-96 with the TopCount system. For better visualization, the upper curve of A was also expanded by a factor of five. When comparing the classical on-line and the new off-line approaches, it becomes clear that curve A shows much more detail than does curve B. In addition to using a 14-fold smaller injection volume, the TopCount/Deepwell LumaPlate-96 combination provides better sensitivity because of a much lower background, while maintaining very high counting efficiency. On a blank Deepwell LumaPlate-96, the background was determined to be only 0-2 CPM. Every count above 4 CPM can be interpreted as a valid tritium signal. It should also be noted that all peak intensities in both curves (A and B) compare well to each other. The main metabolite at a retention time of 16.5 minutes is clearly visible in both curves (A and B). This peak measured by the TopCount shows a better chromatographic resolution than the on-line chromatogram. PRODUCT INFORMATION BULLETIN Technical Information ± 0.25 No - 19 Figure 3: Radioactivity chromatogram of rat urine containing 3H labeled drug; A = 4300 DPM (7 µL) injected and counted with the TopCount system (8 minutes per well). B = 61500 DPM (100 µL) injected and monitored on-line with adding 800 µL LSC-cocktail to a 100 µL mixing cell. Reference: Packard Application Note AN004-TC Worldwide Headquarters: PerkinElmer Life Sciences, 549 Albany Street, Boston, MA 02118-2512 USA (800) 551-2121 European Headquarters: PerkinElmer Life Sciences, Imperiastraat 8, BE-1930 Zaventem Belgium Technical Support: in Europe: [email protected] in US and Rest of World: [email protected] Belgium: Tel: 0800 94 540 • France: Tel: 0800 90 77 62 • Netherlands: Tel: 0800 02 23 042 • Germany: Tel: 0800 1 81 00 32 • United Kingdom: Tel: 0800 89 60 46 Switzerland: Tel: 0800 55 50 27 • Italy: Tel: 800 79 03 10 • Sweden: Tel: 020 79 07 35 • Norway: Tel: 800 11 947 • Denmark: Tel: 80 88 3477 • Spain: Tel: 900 973 255 LumaPlate, TopSeal, MicroMate and Radiomatic are trademarks and TopCount and MicroBeta are registered trademarks of PerkinElmer Life Sciences. www.perkinelmer.com/lifesciences PIB#19 - 1002 - Rev. B © 2002 PerkinElmer Life Sciences