Cocktail Selection for Tetrabutylammonium Hydrogen Sulfate and Acetonitrile Gradients

Discussion
A researcher was counting 1 mL fractions and wished
to add a maximum of 2 mL of a “safer” cocktail to
help comply with local regulations. The sample is a
gradient run from 100% to 45% of solution A, the
remainder is solution B.
We evaluated several safer cocktails, namely
ULTIMA Gold™ XR (part number 6013119), ULTIMA
Gold AB (part number 6013309) and ULTIMA-Flo™
AP (part number 6013599) using this gradient system
and tabulated the results below. The gradient was
evaluated by preparing the mixtures indicated in
the table and for each mixture, 1 mL was added to
2 mL of cocktail. Since no operating temperature
was specified, we checked the stability at 18 °C
and 20 °C only.
Solution A = 3.5 mM tetrabutylammonium
hydrogen sulfate, 35 mM KH2PO4,
0.125 mM EDTA (adjusted to pH 6)
Solution B = 50% of Solution A and 50%
acetonitrile.
Gradient
Ratio A:B
ULTIMA Gold XR
at 20 °C
ULTIMA Gold XR ULTIMA Gold AB ULTIMA Gold AB
at 18 °C
at 20 °C
at 18 °C
ULTIMA-Flo AP ULTIMA-Flo AP
at 20 °C
at 18 °C
100:00
Clear
Clear
Clear
Clear
Clear
Clear
90:10
Clear
Clear
Clear
Clear
Clear
Clear
80:20
Clear
Clear
Clear
Clear
Clear
Clear
70:30
Clear
Clear
Clear
Clear
Clear
Clear
60:40
Clear
Clear
Clear
Clear
Clear
Clear
50:50
Clear
Clear
Clear
Clear
Hazy
Clear
45:55
Clear
Clear
Clear
Clear
Hazy
Clear
Recommendation:
From the table, it can be seen that both ULTIMA Gold
XR and ULTIMA AB are suitable for this application
if used between 18 °C and 20 °C. If tritium is the
nuclide of interest, we recommend the use of ULTIMA
Gold AB since it will give the highest counting
efficiency in this system.
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errors.
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N O T E
Problem
A P P L I C A T I O N
Cocktail Selection for Tetrabutylammonium
Hydrogen Sulfate and Acetonitrile Gradients
L S C C O C K TA I L S
LSC in Practice
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