Dissolving Agarose Gels

Recommendation
A researcher had been trying to use PerkinElmer’s
Soluene®-350 (part number 6003038) to digest
agarose gels.
We recommend the following procedure for reliable
sample preparation and counting of agarose gels:
1. Place a section of agarose gel in a 20 mL glass vial.
Although Soluene-350 has an excellent capacity for the
solubilization of wet tissue, aqueous tissue homogenates,
proteins, nucleotides and high water content biological samples, it cannot dissolve agarose gels.
However, our journals did provide us with an alternate
method that has proven reliable in many laboratories.
It should be noted that techniques utilizing digestion
or solubilization do not allow recovery of the original
sample.
3. Incubate for 45 minutes at 65 °C to dissolve
completely.
4. Allow to cool to room temperature.
5. Add 10 mL of PerkinElmer’s Hionic-Fluor™
(part number 6013319).
* The sodium hypochlorite solution is an aqueous
solution containing about 15% active material
(about 5% to 6% available chlorine). This is
available from any laboratory chemicals supplier.
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or typographical errors.
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N O T E
2. Add 1 mL of sodium hypochlorite solution*.
Discussion
A P P L I C A T I O N
Problem
L S C C O C K TA I L S
LSC in Practice
Dissolving Agarose Gels
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