Alkaline Phosphatase Labeled Affinity Purified Antibody to Rabbit IgG (H+L) [Goat]

TECHNICAL
DATA
SHEET
Labeled Antibodies
Caution: For Laboratory Use. A product for research purposes only
Alkaline Phosphatase Labeled Affinity Purified Antibody to Rabbit
IgG (H+L) [Goat]
Product Number: NEF814001EA
Alkaline Phosphatase Labeled Affinity Purified Antibody to Rabbit IgG (H+L) [Goat]
LOT NO:
SIZE
1.0 mL
DESCRIPTION
Affinity purified antibody, isolated from a pool of serum from goats immunized with purified
rabbit IgG was labeled with phosphatase using a modified glutaraldehyde procedure.
FORM/STORAGE
CONTENT
Liquid. Store refrigerated at 2 – 8°C.
Each vial contains 1.0 mL at 1.0 mg/mL of affinity purified antibody from unlabeled antibody
LOT NO
E/P RATIO
The calculated enzyme/antibody protein ratio:
2.40: 1
SPECIFICITY
Tested by immunoelectrophoresis, gel diffusion and ELISA techniques as applicable. This product reacts
specifically with rabbit IgG, heavy and light chains. No antibody is detectable to non-immunoglobulin serum
components.
SUGGESTED WORKING DILUTIONS
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other
buffer such as blocking solution. These buffers are not recommended for long term storage. Suggested starting
dilutions are as follows. Note: In many cases, the antibody may be diluted more than indicated.
Microwell:
1 / 2,000 – 1 / 10,000
0.5 µg/mL– 0.1 µg/mL
Blotting:
1 / 10,000 – 1 / 40,000
0.1 µg/mL – 0.025 ugImL
Histo / Cytochemical Procedures:
1/400 – 1/1,000
2.5 µg/mL – 1.0 µg/mL
PRODUCT SAFETY AND HANDLING
This product is considered non-hazardous as defined by The Hazard Communication Standard (29 CFR
1910.1200). Avoid contact with skin and eyes. In case of contact or spillage, clean with copious amounts of water.
Dispose via sanitary sewer.
STABILIZERS AND PRESERVATIVES
Goat serum and bovine serum albumin added as protein stabilizer. No preservatives added. Non-sterile.
STABILITY
Product is stable for one year from date of receipt when stored properly.
COUNTRY OF ORIGIN OF ANTISERA
United States
DATE OF MANUFACTURE:
PerkinElmer, Inc.
549 Albany Street
Boston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
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