Cocktail Selection for Use with 3.0 M Ammonium Formate

Introduction
However, they were concerned that the high molarity
of the sample might cause mixing problems and result
in a non-homogeneous counting situation which might
cause problems in their flow counting system.
Sample Volume
Appearance at 20 °C
1.0 mL
Clear
2.0 mL
Clear
4.0 mL
Clear
5.0 mL
Clear
5.5 mL
Clear
6.0 mL
Clear
6.66 mL
Clear
7.0 mL
Clear
8.0 mL
Clear
8.5 mL
Clear
9.0 mL
Hazy/Unstable
10.0 mL
Hazy/Unstable
Recommendation
Discussion
We prepared 3.0 M ammonium formate and buffered
it to pH 4.9 with formic acid and then evaluated
Ultima-Flo AF at 20 °C. The results are shown in
the following table:
Ultima-Flo AF will accept a maximum of 8.5 mL 3.0 M
ammonium formate in 10.0 mL cocktail at 20 °C.
Consequently a flow counting ratio of 2:1 (10.0 mL
cocktail with 5.0 mL sample) is possible with easy,
rapid and reliable mixing.
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007902_20
N O T E
One laboratory was performing flow counting with
samples of 3.0 M ammonium formate, buffered to
pH 4.9 with formic acid. The laboratory requested help
to select the proper safer cocktail from PerkinElmer’s
Ultima-Flo™ family of flow scintillation cocktails.
The researchers suggested that Ultima-Flo AF
(PerkinElmer part number 6013589) might be suitable,
since “AF” indicated that this cocktail had been developed for use with flow gradient systems involving
ammonium formate.
A P P L I C A T I O N
L S C C O C K TA I L S
LSC in Practice
Cocktail Selection for Use
with 3.0 M Ammonium Formate
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