Lot# APC1K110

Technical Data
Caution: For Laboratory Use. A product for research purposes only
Product Number: CR130-100
Product Number:
CR130-100
Compound:
Streptavidin conjugated to SureLight-Allophycocyanin
for kinase assays
Amount:
1.0 mg total protein
Lot Number:
APC1K110
Storage:
4C in the dark. After product has been reconstituted, DO NOT FREEZE.
Handling:
Before use, reconstitute to 1.0 ml with distilled deionized water and allow vial to sit on ice for 20-30 minutes. Gently
mix by vortexing or tapping tube before use.
Buffer:
Supplied lyophilized. Upon reconstitution with 1.0 mL distilled deionized water, the product is in 10 mM Tris (pH
8.2), 150 mM sodium chloride, 50 mM sucrose, 0.1% BSA and 1 g/mL pentachlorophenol.
Stability:
Upon reconstitution with water, product should be stored at 4C and be used within 8 months.
Expiration:
1/2017 when stored lyophilized.
Intended use: The Streptavidin: SureLight-Allophycocyanin conjugate can be used as an acceptor for the energy emitted by Eu 3+-chelates in the LANCE
homogeneous assay format. Product is for research purposes only, the product is not intended for any other use (such as cosmetic, agricultural, drug, food or
household uses). Assay optimization should be based on the Streptavidin concentration. One can assume that 1 Streptavidin binds two biotinylated peptides or
one biotinylated protein.
Lot specific information:
(after reconstitution)
2.00E+06
Concentration: 1.01 mg/ml total protein
Relative Fluorescence
1.60E+06
1.20E+06
SureLight-APC molarity = 4.73 M
8.00E+05
Streptavidin molarity = 9.32 M
4.00E+05
0.00E+00
600
640
680
720
760
Wavelength (nm)
SureLight is a registered trademark of Columbia Biosciences Corporation
LANCE is a trademark of Perkin Elmer Corporation
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