Somatic MASTR Research Assays Simple, fast and robust Next-Generation Sequencing assays for accurate identification and profiling of variants in targeted genes associated with cancer MASTR Technology for Somatic Mutations Profiling Somatic MASTR are molecular research assays, based on multiplex amplification technology, for the identification of variants associated with most human related cancers with Next-Generation Sequencing (NGS). Advances in NGS technologies enable simultaneous sequencing of different cancer-related genes from tumors. The Somatic MASTR research assays are ready-to-use with minimum hands-on time for variant analysis from biopsy tissue, such as formalin-fixed paraffin-embedded (FFPE) samples. Agilent Technologies has developed the following collection of simple, targeted somatic gene research panels to facilitate fast and accurate variant detection from tumors in key targeted genes associated with breast & ovarian, colon, lung, prostate and skin cancer. Tumor Types Cat. No. Product Name Genomic Target Contents Samples Gastrointestinal MR-0150.024 GIST MASTR KIT exons 9,11,13,14,15,16,17 PDGFRA exons 8,10,12,14,18 hotspots (SNV) (17 amplicons) 2 PCR mixes, Taq, AR1 24 Lymphatic MR-0300.024 CLL MASTR Plus TP53, BIRC3, NOTCH1, SF3B1, MYD88, FBXW7, ATM, POT1, XPO1 full exon coverage (SNV, CNA) (251 amplicons) 6 PCR mixes, Taq, AR3 24 Lung MR-0130.024 EGFR 18-21 MASTR EGFR exons 18 - 21 hotspots (SNV) (4 amplicons) 1 PCR mix, Taq, AR1 24 Gastrointestinal, glioblastomas, colon, lung, breast, ovarian, endometrial, and other cancers MR-0200.024 Tumor Hotspot MASTR Plus 26 cancer related genes hotspots (SNV) (252 amplicons) 4 PCR mixes, Taq, AR1 24 Pan cancer MR-0160.024 TP53 MASTR full exon coverage (SNV) (17 amplicons) 2 PCR Mixes, Taq, AR2 24 Workflow NGS Sample Preparation Multiplex PCR Universal PCR Pooling & Clean Up Quality Control www.agilent.com Not for EU [email protected] For Research Use Only. Not for use in diagnostic procedures. This information is subject to change without notice. PR7000-1435 © Agilent Technologies, Inc. 2018 Published in the USA, March, 2018 5991-8392ENUC SEQUENCING DATA ANALYSIS