HP G7555B Sureguide crispr library Datasheet

SureGuide CRISPR Libraries
GeCKOv2 Catalog Libraries
Benefits
SureGuide CRISPR Libraries
SureGuide Catalog CRISPR Libraries
Agilent’s pooled libraries come in a variety of formats designed to suit your unique
experimental requirements. Pooled libraries require both uniform representation
and high fidelity to reduce screening, improve analysis and speed your time to
results. All of our libraries, whether standard, genome-wide content or fully
customized pools, are synthesized on our industry-leading DNA manufacturing
system, so you can be sure of getting the same quality and consistency out
of every assay. Our CRISPR libraries are also one of the few licensed solutions
for conducting research with pooled guide libraries, making sure that you have
not only the best libraries available but the freedom to use them for the research
you’ve designed.
– Exome-wide knock-down screening Validated guides targeting all known
genes
– Highest quality, sequence-verified
GeCKOv2 libraries
– Lentiviral, ready-to-package plasmid
libraries
– Human and mouse libraries - Common
model organisms/cell lines for genomewide screening
– Full flexibility to develop your research
applications
Catalog Ready-to-Package Plasmid Library
Agilent’s catalog CRISPR guide libraries target all exonic sequences in the human
and mouse genome and are ideal for genome-wide functional screening. These
libraries contain content developed by and licensed by a well known research
institute. In comparison to other GeCKO libraries, Agilent’s GeCKO libraries
contain fewer missed guides and more uniform representation across the
library (Table 1). Both the mouse and human GeCKO libraries come as plasmids,
with a puromycin selection marker and a GFP reporter, that can be used to
generate high-titers of lentiviral particles.
Integration of SureGuide CRISPR library in Genomics Assay Workflow
OLS Library
PCR amplify
Clone into
viral vector
Transform
E. coli host
Isolate
plasmid DNA
Transfect
packaging line
Catalog Ready-to-Package Library
Custom Ready-to-Clone Library
Custom Ready-to-Amplify Library
Figure 1. Agilent offers CRISPR libraries in a variety of formats, from plasmid libraries to unamplified oligo pools.
Isolate viral
supernatant
GeCKO Libraries
Guide Representation Comparison with Competitor Libraries
Species
Human
Mouse
Genes targeted
19,050
20,611
6
6
1,864
1,175
4
4
Targeting constructs per gene
miRNA targeted
Targeting constructs
per miRNA
Control (nontargeting) sgRNA
Total sgRNA constructs
Viral plasmid vector
1,000
1,000
123,411
130,209
Single and dual vector.lentiCRISPRv2
and lenitGuide-Puro
Missed
Guides
90th/10th
percentile
95th/5th
percentile
99.5th/0.5th
percentile
Agilent option 1
1
2.32
3.04
6.72
Agilent option 2
1
2.47
3.38
11.06
Agilent option 3
1
2.38
3.19
9.83
Agilent option 4
1
1.99
2.64
8.30
GeCKO (Broad)
?
8.73
16.00
NA
GeCKO (Competitor)
39
5.29
9.83
68.40
GeCKO (Competitor) expanded
204
6.00
11.95
333.00
Table 2. With <.01% missed guides, you can be sure the guides in your library
will be present in your delivered library. The uniformity of our libraries has an
average 90/10 ratio of 2.29, which is less than half that of our competitor’s
libraries (5.29).
Table 1. The GeCKOv2 library contains experimentally validated CRISPR
guides targeting all human or mouse genes.
Instructions to order GeCKOv2 Libraries
GeCKOv2 libraries are available for both mouse (G7554A) and human (G7555A) applications. Table 1 provides a summary of
the libraries including the number of guides per gene, non-coding targets, and control sequences. Kits also include a control
appropriate for the organism the library is designed for. If you require additional information, including the sequences of each
component of the library please contact your local Agilent representative or technical support.
Ordering Information
SureGuide GeCKOv2
CRISPR Libraries
SureGuide Amplified Custom
CRISPR Libraries
SureGuide Unamplified Custom
CRISPR Libraries
Part Numbers
G7553A - Human
G7554A - Mouse
G7555A - Human (hU6 promoter)
G7555B - Any species
Library Format
Ready-to-package plasmid library
Ready-to-clone amplified DNA library
Ready-to-amplify DNA library
Delivery
Lentivirus
Designed for lentivirus, user choice
Users choice
Number of Guides
Human - 123,411; Mouse - 130,209
Up to 60,000
Up to 100,000
User Supplies
Lentivirus packaging, delivery, screening
Cloning, viral packaging, delivery screening
Amplification, cloning, viral packaging,
delivery, screening
Sequence Verified
Yes
No
No
Quantity
200 ug of plasmid DNA split into A and B libraries
125 ng of linear, amplified, pooled library
10 pmol of linear, unamplified, pooled
library
Also Included
N/A
N/A
Amplification kit
Custom Design Input
None
Up to 50 nt sequence for each guide
Complete sequence up to 200 nt
Control
Mal1-C
User designed
User designed
www.agilent.com/genomics/CRISPR
For Research Use Only. Not for use in diagnostic procedures.
This information is subject to change without notice.
PR7000-0245
© Agilent Technologies, Inc. 2017
Published in the USA, September, 2017
5991-7347EN
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