HP MR-0130.024 For detection of germline variants, snvs, in exons 18-21 of egfr in ffpe-derived dna Datasheet

EGFR 18-21 MASTR
The EGFR MASTR is a molecular research assay for the identification of variants in exons
18 to 21 of the EGFR gene, which codes for the Epidermal Growth Factor Receptor (EGFR).
This assay is ready to use and offers robust performance with minimum hands-on time. It
consists of all the reagents necessary to enable multiplex amplification of 4 amplicons for
complete coverage of exons 18 to 21 of the EGFR gene.
EGFR 18-21
Research application
Performance Characteristics
Assay characteristics
• For detection of germline variants, SNVs, in exons 18-21 of EGFR in FFPE-derived DNA.
Genes
Full coding region of exons 18-21 of EGFR
Genomic region analyzed
0.6 kb
Number of amplicons
4
Amplicon length
100-180 bp
Number of plexes
1
Designed to be compatible with
MiSeq, MiniSeq, NextSeq, Ion Torrent NGS Systems
DNA input
as low as 4 ng per plex reaction
Number of samples/run**
(20 reads/allele):
@ 5 % VAF sample
Illumina MiSeq V2: 2,057*
Illumina MiSeq V3: 3,771*
* only 192 MID combinations available.
** Number of samples per run for Illumina & IonTorrent NGS Systems can be calculated via the Sequencing Calculator.
Order
information
Workflow
MASTR workflow
Multiplex PCR
Universal PCR
Dilution
Mixing Clean up
Pooling
NGS
SEQUENCING
Plan run
Multiplex PCR
Quality Control
Universal PCR
Quality Control
Mixing plexes of
single sample
Purification
DATA ANALYSIS
Equimolar pooling
Cat. No.
Product Name
Reactions
MR-0130.024
EGFR 18-21 MASTR
1
MID (Molecular Identifiers) kits are necessary to complete the workflow.
For Research Use Only. Not for use in diagnostic procedures.
© Agilent Technologies, Inc. 2017
Printed in Belgium, December 31, 2017
5991-8383ENN
PR7000-1408
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