TP53 MASTR The TP53 MASTR is a research panel for the identification of variants in the coding region of TP53, a gene frequently mutated in a range of cancers. TP53 Research application • For detection of mutations (SNVs and small indels) in TP53 in FFPE-derived DNA • For detection of mutations (SNVs and small indels) in TP53 in blood-derived DNA • For detection of mutations (SNVs and small indels) in TP53 in FFT-derived DNA Panel characteristics 17 Gene full coding region of TP53 (excl. promoter 5’ or 3’ UTR) Genomic region analyzed 2.4 kb Number of amplicons 17 Amplicon length 176 - 260 bp Number of plexes 2 Design compatible with MiSeq, NextSeq, MiniSeq and Ion PGM Systems Number of samples/run @ 5 % VAFsample: Illumina MiSeq V2: 211* Illumina MiSeq V3: 388* * only 192 MID combinations available. Due to the low complexity of the library, it is advised to combine with other assays on a sequencing run Performance Parameters ** Number of samples per run for Illumina & IonTorrent NGS Systems can be calculated via the sequencing calculator. Uniformity of amplification (≥ 0.2 x mean coverage) ≥ 99 % amplicons on high-molecular weight DNA ≥ 96 % amplicons detected on DNA from FFPE tissue On target read count >93 % for FFPE-derived DNA Low DNA amount 4 ng per plex reaction Lowest observed mutation frequency 3% Order information Workflow MASTR workflow Multiplex PCR Universal PCR Dilution Mixing Clean up Pooling NGS SEQUENCING Plan run Multiplex PCR Quality Control Universal PCR Quality Control Mixing plexes of single sample Purification DATA ANALYSIS Equimolar pooling Cat. No. Product Name Samples MR-0160.024 TP53 MASTR 24 MID (Molecular Identifiers) kits are necessary to complete the workflow. For Research Use Only. Not for use in diagnostic procedures. © Agilent Technologies, Inc. 2017 Printed in Belgium, December 31, 2017 5991-8391ENN PR7000-1416 Multiplicom N.V. [ a ] Galileïlaan 18, B-2845 Niel, Belgium [ w ] www.agilent.com [ e ] [email protected] [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16