TECHNICAL DATA SHEET AlphaScreen® Research Reagents Caution: For Laboratory Use. A research reagent for research purposes only TruHits Kit Product No.: 6760627D/M Lot No.: 1583439 Material Provided Format: 6760627D 6760627M 1000 Assay Points 10000 Assay Points Note: The number of assay points is based on a final bead concentration of 20 µg/mL in a 25 µL/well reaction volume. Manufacturing Date: February 08, 2012 Kit Components: Component BSA-biotin Acceptor Beads at 2.5 mg/mL in 1X PBS, 0.05% Proclin-300, pH 7.2 Streptavidin Donor Beads at 5 mg/mL in 1X PBS, 0.05% Proclin-300, pH 7.2 6760627D 1 x 50 µL 6760627M 1 x 500 µL (6760148B) (6760149) 1 x 50 µL 1 x 500 µL (6760003) (6760003C) Product Information Antibody/Protein: The biotinylated Acceptor beads are coated with biotinylated bovine serum albumin (BSA) designed as a tool for AlphaScreen users to identify false positives in AlphaScreen HTS assays Stability: This product is stable for at least 12 months from the manufacturing date if used and stored under recommended conditions. Storage Conditions: Store undiluted at 4°C protected from light. Freeze-thaw is not recommended and can cause the beads to form aggregates. Recommended use: AlphaScreen® Donor beads are light-sensitive. All Alpha assays using the Donor beads should be performed under subdued laboratory lighting (< 100 lux). Green filters (LEE 090 filters (preferred) or Roscolux filters #389 from Rosco) can be applied to light fixtures. ® For additional information on running AlphaScreen assays or on potential interfering compounds, please visit our website: www.perkinelmer.com/AlphaTech TDS-6760627D/M-02 Page 1 of 2 Quality Control ® ® AlphaScreen maximum signal, minimum signal are based on a one point assay performed on an EnVision HTS Alpha detection instrument. Maximum signal represents an average of the 18 wells. We certify that these results meet our requirements. TEST RESULTS Maximum signal Background 259 956 cps 17 cps Recommended Assay Conditions Note: This protocol provides a method to verify kit performance and is not representative of an assay. Acceptor Beads: Add 10 μL BSA-biotin Acceptor beads to 990 μL 1x PBS, pH 7.2. Donor beads: Add 10 μL Streptavidin Donor beads to 990 μL 1x PBS, pH 7.2. Premix: Pre-mix the BSA-biotin Acceptor beads and Streptavidin Donor beads from previously diluted solutions by adding 100 µL BSA-biotin Acceptor beads to 100 µL Streptavidin Donor beads and 300 uL of 1x PBS, pH 7.2. Incubate in the dark at room temperature for 30 minutes. Titration Protocol: To a white opaque 384-well Optiplate: 1- Add 25 μL in 18 wells. Incubate in the dark at room temperature for 10 minutes and analyze on your AlphaScreen reader. ® detection Suggested Materials and Instrumentation Please visit our website www.perkinelmer.com/AlphaTech This product is not for resale or distribution except by authorized distributors. LIMITED WARRANTY: PerkinElmer BioSignal Inc. warrants that, at the time of shipment, the products sold by it are free from defects in material and workmanship and conform to specifications which accompany the product. PerkinElmer BioSignal Inc. makes no other warranty, express or implied with respect to the products, including any warranty of merchantability or fitness for any particular purpose. Notification of any breach of warranty must be made within 60 days of receipt unless otherwise provided in writing by PerkinElmer BioSignal Inc. No claim shall be honored if the customer fails to notify PerkinElmer BioSignal Inc. within the period specified. The sole and exclusive remedy of the customer for any liability of PerkinElmer BioSignal Inc. of any kind including liability based upon warranty (express or implied whether contained herein or elsewhere), strict liability contract or otherwise is limited to the replacement of the goods or the refunds of the invoice price of goods. PerkinElmer BioSignal Inc. shall not in any case be liable for special, incidental or consequential damages of any kind. PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2009, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.