FLOW CELLS OVERVIEW Hamamatsu provides a wide variety of precision glass products such as flow cells. These are designed and manufactured based on our 60 years of experience in the production of vacuum tubes such as photomultiplier tubes along with our well-established glass processing technology. We welcome your requests for custom products. -003 -002 FEATURES ●High dimensional accuracy: Minimum tolerance ±0.005 mm ●Available in various shapes (polishing, grinding, gas processing, lens bonding) ●Materials: synthetic fused silica, synthetic black quartz, etc. ●Custom products with sample inlet/outlet mechanism available -004 -001 -006 -005 APPLICATIONS ●Flow cytometry ●Blood analysis ●Imaging flow cytometry ●Particle counter SPECIFICATIONS Type No. Material Flow channel size (mm) J11020-000-001 Standard type. Suitable flow channel for detecting minute particles. -002 Grinded type. Standard type with nipple-type outlet. M0.25 ± 0.025 -003 Synthetic fused silica -004 Trapezoid *2 -005 -006 Features Synthetic fused silica *1 Synthetic black quartz M1.0 ± 0.025 Standard type flow cell with a synthetic fused silica hemisphere lens attached by UV glue. Combined glass pipe with flow cell by gas-process. Smooth laminar flow and hydrodynamic focusing. Plate type. Synthetic black quartz galss type. Less stray light and low fluorescence from material. *1: Index of refraction of synthetic fused silica: nC (656 nm) 1.456, nd (587 nm) 1.458, nF (486 nm) 1.463 *2: Upper base: 0.2 mm m ±0.03 mm, Lower base: 0.15 m mm ±0.03 03 mm, Height: 0.2 mm ±0.03 mm Customization options: Size, shape, etc. Customization With grinding 1 With grinding 2 High quality synthetic fused silica flow cells With step Large channel Thin cover glass Synthetic black quartz With lens Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2016 Hamamatsu Photonics K.K. FLOW CELLS ●Surface quality Parameter Optical active areas or flow channel Other polished surfaces Scratch Width 0.004 mm Max. Width 0.02 mm Max. Dig Size 0.025 mm Max. Size 0.1 mm Max. Bubble and inclusion Size 0.025 mm Max. Size 0.1 mm Max. Chipping Width 0.025 mm Max. Width 0.1 mm Max. Stain Size 0.01 mm Max. Size 0.1 mm Max. ●Dimensional outline (Unit: mm) J11020-000-001 J11020-000-002 5 ± 0.1 15 ± 0.1 1 9 ± 0.1 A 1 ± 0.1 2 × EFFECTIVE AREA 1.5 6 0.25 ± 0.025 2 × 0.22 DETAIL A TPMZA0005EC J11020-000-003 TPMZA0006EC J11020-000-004 5 ± 0.1 15 ± 0.1 R2.5 (LENS) 9.5 2.475 FLOW CHANNEL 2 × 0.22 FLOW CHANNEL DETAIL A 8 ± 0.5 GLUED JOINT 35 ± 0.5 5 ± 0.5 A 4 ± 0.05 (6) 11 ± 1 2 × 0.22 60° 1 2 × EFFECTIVE AREA (5) 2 × EFFECTIVE AREA 0.25 ± 0.025 1 0.22 6 2 4.475 ± 0.1 2 × EFFECTIVE AREA 2 × EFFECTIVE AREA 2 × EFFECTIVE AREA 0.25 ± 0.025 2.5 (6) 60° 4 ± 0.1 60° 4 ± 0.1 A 15 ± 0.1 9 2 × 0.22 5 2 × 0.22 5 ± 0.1 FLOW CHANNEL A 4 × EFFECTIVE AREA 0.25 ± 0.025 FLOW CHANNEL DETAIL A DETAIL A TPMZA0007EC TPMZA0008EC J11020-000-006 SYNTHETIC BLACK QUARTZ 47 5.0 ± 0.1 4.0 ± 0.1 5.5 EFFECTIVE AREA 0.2 ± 0.03 4 16 MIN. A 9 FLOW CHANNEL 2 × AREA SYNTHETIC BLACK QUARTZ 16 MIN. (0.2) TPMZA0014EB 0.2 ± 0.03 24 15.0 ± 0.1 5 26 1 ± 0.025 A SYNTHETIC BLACK QUARTZ 1 55 2 2.2 1 ± 0.025 J11020-000-005 0.15 ± 0.05 EXPANSION A-A TPMZA0009EC ●Other capabilities Mirror coating Transmittance of synthetic black quartz Ex.: Aluminum mirror: reflectance characteristics 100 TPMZ0022EA TPMZ0023EA 10 THICKNESS 0.3 mm 0.5 mm 0.7 mm 1.0 mm 80 70 FROM AIuminum SIDE FROM AIuminum SIDE With SiO2 COAT FROM GLASS SIDE 60 50 40 30 20 TRANSMITTANCE (%) Typ. REFLECTANCE (%) Typ. 90 1.0 0.1 10 0 200 300 400 500 600 700 800 900 0 200 300 WAVELENGTH (nm) 400 500 600 700 800 WAVELENGTH (nm) * The values on the above chart are sample values only * The values on the above chart are sample values only Optical thin film coating Marking Ex.: AR (Antireflection) coating: reflectance characteristics 5.0 <0.5 % @488 nm <1.0 % @400 to 650 nm 4.5 REFLECTANCE (%) Typ. ● Serial number ● Part number ● Flow path diameter TPMZ0024EA 4.0 405 nm 3.5 633 nm 488 nm 532 nm 3.0 2.5 2.0 1.5 1.0 0.5 0 350 400 450 500 550 600 650 700 WAVELENGTH (nm) * The values on the above chart are sample values only Clear polishing for grinded inner surface Fusion welding Ex.: Conical shape / 30 deg Ex.: J11020-000-004 Ex.: Micro cylindrical / 0.5 mm diameter FLOW CELLS FLOW CELL ASSEMBLY J12800 SERIES FEATURES ●Alignment free ●Can use commercial tube fitting ●Easy to install ●Repetition stability ●Stable laminar flow TECHNOLOGIES ▲J12800-000-101 ●Proposed assembly structure design based on laminar flow model ●Flow simulation technology ●High pressure toughness ▲J12800-000-103 SPECIFICATIONS Parameter Flow channel Outside dimension Material Outside dimension Accuracy of position Fitting of sheath port Fitting of the other ports Control pressure Flow cell Assembly Unit µm mm mm µm inch inch kPa J12800-000-101 J12800-000-103 Please refer to 250 × 250 J11020-000-005 20.0 (H) × 5.0 (W) × 4.0 (D) Synthetic fused silica 65.0 (H) × 46.0 (W) × 28.7 (D) 137.0 (H) × 59.0 (W) × 19.0 (D) — ± 100 for 1/16ID tubing for 1/8ID tubing for 1/16ID tubing -50 to +200 +10 to 70 ●Dimensional outline (Unit: mm) J12800-000-103 28.7 10 5 BARB FITTING FOR 1/8”ID TUBING IDEX: P-647/ETFE (1/4-28 MALE SCREW) 4 × 3.5 THRU HOLE 3x BARB FITTING FOR 1/16”ID TUBING IDEX: P-646/ETFE (1/4-28 MALE SCREW) 19 10 54 40 A 14 +0.00 FLOW CELL 7.8 5 55 2.2 E 13 14 1/16”OD, 0.15 mmID NEEDLE / STAINLESS STEEL 26 10 90° 4 HOLES FOR MOUNTING (M3 or #5-40UNF) 10 (137) (121.9) 4 × M3 THREADED HOLE NOTES 1. Pressure resistance: 30 psi 2. Easy to assemble and disassemble for cleaning, etc. 24 14 65.5 69 SAMPLE INLET PORT 71.5 28 28.4 12 SHEATH INLET PORT E 46 36 60.00 - 0.05 WASTE INLET PORT SECTION A-A 17 28 59 13.5 5.00 ± 0.05 FITTING FOR 1/16”OD TUBING IDEX: P-420/PEEK FEMALE NUT, P-250/PEEK FERRULE HAMAMATSU PHOTONICS K.K. 4.00 ± 0.05 0.25 ± 0.025 0.250 ± 0.025 2.00 ± 0.025 REFERENCE DRAWING OF J11020-000-005 0.1 A F 27.0 ± 0.10 13 21 31 2.50 ± 0.025 8.00 ± 0.05 0.1 A A 26 65 14 11.5 J12800-000-101 A B 120° FLOW CELL SYNTHETIC FUSED SILICA: TOSOH ES www.hamamatsu.com HAMAMATSU PHOTONICS K.K., Electron Tube Division 314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205 U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, United Kingdom, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] TPMZ1011E03 Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-93581733, Fax: (39)02-93581741 E-mail: [email protected] JAN. 2016 IP China: Hamamatsu Photonics (China) Co., Ltd.: B1201 Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected]