S P E C I F I C A T I O N S Radiometric and Luminescence Detection MicroBeta2 and MicroBeta2 LumiJET Microplate Counters Description MicroBeta®2 and MicroBeta2 LumiJET are microplate counters that measure radioactivity, as well as luminescence. These instruments are available in 1-, 2-, 6-, and 12-detector configurations with 16-shelf loading capacity. A robotic loading option allows efficient integration into robotics systems. Each instrument model also supports different plate matrices and is capable of running samples in either “96-well + 384-well plate” format or in “96-well + 24-well plate” format. The samples can be contained on microtiter plates, 4-mL vials, Eppendorf® tubes, or on filtermats. The MicroBeta2 LumiJET model is available with a choice of single or dual injectors per detector, and is also available with 1-, 2-, 6-, and 12-detector configurations. Both 96- and 384-well plates can be used. The injector configuration of the MicroBeta2 LumiJET enables flash luminescence studies. MicroBeta2 MicroBeta2 LumiJET Standard Features for MicroBeta2 Plate Counters • LCD display: 17” Hardware • 1,2, 6, or 12 detectors • Keyboard and mouse • LAN connectivity •1- and 2-detector systems run 24- and 96-well plates, 6- and 12-detector systems run 96- and 384-well plates, 6-detector systems can be configured to count 24- and 96-well plates (optional) • Sample types for radioactivity: – Liquid scintillation counting in microtiter plates – Filterplates, e.g. UniFilter Plates ® – Filtermats – FlashPlates® – Solid scintillator, e.g. MeltiLex® – Scintillation proximity assay Manager Software •Factory preset labels exist in software. User may manually add new isotopes to the library. •Plate library contains SBS standard plate settings. The user may specify non-standard plate settings in the system. •Repeat, replicates, and cycles can be programmed into the protocol. •Delays can be set before the measurement, between cycles, and between plates. – Microtiter plates, e.g. OptiPlate™ or CulturPlate™ •Plate mapping is used to define the positions of the measurable samples. Protocol-specific “auto-fill” features allow quick and easy set up of sample positions in a plate map. •Counting modes include single- and dual-label CPM, single- and dual-label DPM, ParaLux™ count mode, and luminescence counting. •Optional Enhanced Security functionality to support 21 CFR Part 11 compatibility. Includes audit trails, access control, and data security features. • Proprietary Time-Resolved Liquid Scintillation Counting (TR-LSC) mode significantly improves counting capabilities with opaque plates and low-energy isotopes. TR-LSC mode can generate close to dual-PMT coincidence counting performance by using top PMTs only. Data Collection •Unique detector design consists of two photomultiplier tubes (PMT). One is located below the sample, the other above the sample. These count the sample simultaneously, in coincidence, which allows the best possible counting geometry, superior counting efficiency, and the most efficient reduction of background. •Counting commands, such as "Next position," "Next assay," and "Stop" are available while the counter is operating. • Sample types for luminescence: •PMT positioning for the measurement is automatic. Maximum height of the sample for 16-shelf MicroBeta2 is 45 mm, or 20 mm with robotic loading shelf. •Robust cassette sample-changing mechanism provides a flexible system for varying sample types. Microtiter plates, micro centrifuge tubes, 4-mL liquid scintillation counting (LSC) vials, and Filtermats can be loaded simultaneously into the instrument. •Standard barcode reader allows recognition of 100 protocols and other counting commands. •Luminescence option includes a cooling device, which assures a stable temperature for the Upper PMTs, providing superior luminescence sensitivity. Computer Specifications •User interface: Windows® 7 (32 bit) •Processor: 1.6 GHz •RAM: 4 GB •Hard Disk Drive: 240 GB 2 •Extra USB ports •Live display includes instrument status and allows the user to follow the measurement while running the assay. •Numeric or color intensity display for 96- and 24-well plate formats. •Count termination is determined by fixed time or counting precision. •Up to 100 normalizations can be performed and linked into 100 counting protocols to provide detector efficiency corrections, background corrections, and crosstalk correction for the final data. •ParaLux count mode for scintillation proximity assays fully utilizes the advantage of twin photomultiplier tubes. Compared to all other methods, counting efficiency is increased by up to 500%. The ultra-sensitive, high dynamic range Asymmetric Quench Parameter AQP(I) provides superior disintegrations per minute (DPM) calculations. •Easy DPM includes pre-stored quench data that is modified by measuring only two standard samples. This allows quick access to DPM data without quench correction. •DPM monitor reports samples outside the range of the quench curve. •Up to 100 quench corrections can be performed and linked into 100 counting protocols. Quench corrections will also include detector efficiency corrections, background corrections, and crosstalk corrections. •Password protection for counting, normalization, and quench correction protocols are included. Analysis •Three counting windows allow the analysis of the signal in three independent counting regions. •Background subtraction enables fixed samples or values obtained from detector normalization to be used for calculations. •Half-life correction is a correction facility for CPM and DPM values. The zero time may be the start time of the assay or a specified date and time. •Freely selectable data output options include quench parameters (SQP(I)), spectrum plot, date and time, CPM and DPM monitors, and statistical analyses. Output data format can be customized to either plate or list format. •Instrument Performance Assessment (IPA) allows the user to monitor the performance of the instrument with the standard samples. The user may store the data for later analyses. • Results output: – Samples in either column or plate format, or both – Automatic file run numbers are generated to avoid the loss of data by overwriting – File names can combine several identifiers, such as counter name, protocol owner, protocol name, protocol number, or plate index Additional Features for MicroBeta2 LumiJET Hardware In addition to all features in the above MicroBeta2 section, MicroBeta2 LumiJET has additional features: •Standard 1- and 2-detector systems inject into 24- and 96-well plate formats. •Standard 6- and 12-detector systems inject into 96- and 384well plates. For 6-detector model, additional option converts plate format into 24- and 96-well formats. •Depending upon the dispenser model, up to two reagents can be dispensed into a single well. Injection volume and speed are user-selectable. – Result file may be saved as ASCII or CSV file Available Configurations MicroBeta2 MicroBeta2 LumiJET Model Number of Detectors Plate Capacity Plate Format Model Number of Detectors Plate Capacity Plate Format 2450-0010 1 16 24/96 2460-0010 1 16 24/96 2450-0020 2 16 24/96 2460-0020 2 16 24/96 2450-0060 6 16 96/384 2460-0060 6 16 96/384 2450-0120 12 16 96/384 2460-0120 12 16 96/384 *Optional robotic loading system available. To complete LumiJET system a dispensing device needs to be ordered separately. Typical Performance Data Physical Data Liquid Scintillation Counting Unquenched sample with a volume of 150 μL of cocktail unpurged, in a flexible 96-well microtiter plate: 3 Counting efficiency: H Typically 57 % 14 C Typically 94 % Maximum count rate: 3,000,000 CPM Dimensions for MicroBeta2: Height: 609 mm (24.0 in.), except 2450-0320 which is 1207 mm (47.5 in.) Width: 433 mm (17.0 in.) Depth: 645 mm (25.4 in.) Weight: 85 kg (187.4 Ib.) model 2450-0010 – 90 kg (198.2 lbs.) Dispensing Performance (LumiJET models) Adjustable dispensing speed and volume Dispensing volume: 5-250 μL Dispensing accuracy: 1 μL or 5 % (whichever is larger) Dispensing precision (CV%): 5 μL – 2 % 25 μL – 1 % 250 μL – 0.5 % Luminescence Counting Luminescence samples in a white 96-well OptiPlate, 200-μL sample volume Background: 100 CPS Maximum count rate: 24,000,000 CPS Crosstalk: 0.002 % Dimensions for MicroBeta2 LumiJET Height: 630 mm (24.8 in.) Width: 700 mm (27.56 in.) Depth: 660 mm (25.7 in.) Weight: 90 kg (198.4 Ib.) Electrical requirements (both models): Main voltage selectable 100, 115, 120, 240 V +/- 10 % Frequency 50/60 Hz Power consumption 360 VA max 3 Safety, Radiated Emissions, and Immunity: MicroBeta2 and MicroBeta2 LumiJET have been tested and approved for electrical safety, radiated emissions, and electromagnetic compatibility. In the USA, the CSA approval also satisfies the requirements of 29CFR 1910.399. MicroBeta2 and MicroBeta2 LumiJET conform to the following EU directives: CE marking: – 2004/108/EC Electromagnetic compatibility – 73/23/EEC (as amended by 2006/95/EC) Low Voltage MicroBeta2 and MicroBeta2 LumiJET fulfill the requirements of the following standards: – IEC 61010-1:2001 (Second Edition) – CAN/CSA-C22.2 61010-1:2004 – UL 61010-1:2004 R7.05 PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2008-2016, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 008380A_02PKI