TECHNICAL DATA SHEET Light Producing Microorganisms Caution: For Research Use. This product is intended for animal research only and not for use in humans. Not for human or animal therapeutic or diagnostic use. Staphylococcus aureus S. aureus ATCC 12600 (Xen29) Product No.: 119240 Material Provided: 1 Agar Plate Storage Conditions: -80°C In vitro Characteristics Genetic Characteristics Staphylococcus aureus Xen29 was derived from the parental strain S. aureus 12600, a pleural fluid isolate, which is also designated as NCTC8532. S. aureus Xen29 possesses a stable copy of the modified Photorhabdus luminescens luxABCDE operon at a single integration site on the bacterial chromosome. Growth Characteristics S. aureus Xen29 grows well in various media including Luria Bertani (LB), Brain Heart Infusion (BHI), and Nutrient Broth (NB) at 37ºC under ambient aeration. S. aureus Xen 29 may also be grown selectively on medium containing 200 μg/ml kanamycin. Colonial Morphology On LB agar, S. aureus Xen29 appears as small (~1.5mm), cream-colored, opaque, smooth, circular colonies. Virulence Factor Hemolysis: β-hemolysis on TSA + 5% sheep blood Capsule: Serotype 3 (NCTC type strain). DNAse: Positive. 119240-Xen29-Rev-2 NaCl: Tolerant via growth on Mannitol Salts Agar. Coagulase: Positive in 24hrs. mecA: negative Growth Curve Log-phase growth can be achieved after 2 to 3 hours of subculture in LB broth at 37ºC, shaking at 200rpm. An absorbance measurement at 600nm (against a LB blank) of 0.5 is roughly equivalent 8 to 1.44x10 cfu/ml of S. aureus Xen 29. Biochemical Profile A biochemical profile was obtained for S. aureus Xen 29 using the api 20 STAPH system available from bioMérieux. Sugar Utilization D-Glucose D-Fructose D-Mannose Maltose Lactose Trehalose D-Mannitol + + + + + + + Xylitol - Raffinose - Xylose - D-Melibiose Sucrose + + Other Tests Nitrate Reduction Alkaline Phosphatase Voges Proskauer α- methyl-D-glucoside N-acetyl-glucosamine Arginine dihydrolase Urease NCCLS Macrodilution MIC/MBC + + + + + Resistant to: Kanamycin References J Biomed Mater Res B Appl Biomater. July 2008 Microbes and Infection, Vol. 10, Issue 3, March 2008, 217-223 J Orthop Res. 2008 Jan; 26(1):96-105 J Biol Phys 2008 Vol. 33:61–66 Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794 Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794 Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794 PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com MIC (μg/mL) 8.0 4.0 2.0 R up to 16 0.125 0.5 MBC (μg/mL) 32 8.0 >16* n/a 0.25 >16 Product Information Kirby-Bauer Disk Diffusion Test 1. 2. 3. 4. 5. 6. 7. Antibiotic Ceftriaxone Ciprofloxacin Erythromycin Gentamicin Penicillin G Tetracycline * trailing endpoint. Antibiotic Susceptibility Disk Diffusion Data: Disk diffusion tests were performed according to methods outlined in the NCCLS Approved Standard M2-A7. Sensitive to: Carbenicillin 100 Gentamicin 20 Penicillin G 10U Vancomycin 30 MIC and MBC Data MIC and MBC were determined using the macrodilution methods specified in the NCCLS Approved Standard M7-A5. Warranty PerkinElmer warrants that cells will be viable upon shipment from PerkinElmer for a period of thirty days, provided they have been properly stored and handled during this period. Disclaimers This product is sold for in vivo animal research use only and is not for use in any diagnostic procedures. Excluding purchases by authorized PerkinElmer distributors, this product is sold for use by the original purchaser and is not for resale. 8. Antimicrobial Agents & Chemotherapy, May 2007, 51(5) 1787-1794 9. Photochem. Photobiol. Sci., 2006, 5, 31 – 38 10. Journal of Antimicrobial Chemotherapy, Apr. 2005, Vol. 55, No. 4, 528-534 11. Antimicrobial Agents & Chemotherapy, June 2004 2283-2287 12. Antimicrobial Agents & Chemotherapy, June 2004, Vol. 48, No. 6, 2283-2287 13. Infection & Immunity, Feb. 2003, Vol. 71, No. 2, 882-890 14. Antimicrobial Agents & Chemotherapy, Oct. 2003, Vol. 47, No. 10, 3130-313 For more information, please visit our website: ww.perkinelmer.com/microorganisms. For a complete listing of our products, visit www.perkinelmer.com. Copyright ©2015, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 119240-Xen29-Rev-2