EGFR 18-21 MASTR The EGFR MASTR is a molecular research assay for the identification of variants in exons 18 to 21 of the EGFR gene, which codes for the Epidermal Growth Factor Receptor (EGFR). This assay is ready to use and offers robust performance with minimum hands-on time. It consists of all the reagents necessary to enable multiplex amplification of 4 amplicons for complete coverage of exons 18 to 21 of the EGFR gene. EGFR 18-21 Research application Performance Characteristics Assay characteristics • For detection of germline variants, SNVs, in exons 18-21 of EGFR in FFPE-derived DNA. Genes Full coding region of exons 18-21 of EGFR Genomic region analyzed 0.6 kb Number of amplicons 4 Amplicon length 100-180 bp Number of plexes 1 Designed to be compatible with MiSeq, MiniSeq, NextSeq, Ion Torrent NGS Systems DNA input as low as 4 ng per plex reaction Number of samples/run** (20 reads/allele): @ 5 % VAF sample Illumina MiSeq V2: 2,057* Illumina MiSeq V3: 3,771* * only 192 MID combinations available. ** Number of samples per run for Illumina & IonTorrent NGS Systems can be calculated via the Sequencing Calculator. Order information Workflow MASTR workflow Multiplex PCR Universal PCR Dilution Mixing Clean up Pooling NGS SEQUENCING Plan run Multiplex PCR Quality Control Universal PCR Quality Control Mixing plexes of single sample Purification DATA ANALYSIS Equimolar pooling Cat. No. Product Name Reactions MR-0130.024 EGFR 18-21 MASTR 1 MID (Molecular Identifiers) kits are necessary to complete the workflow. For Research Use Only. Not for use in diagnostic procedures. © Agilent Technologies, Inc. 2017 Printed in Belgium, December 31, 2017 5991-8383ENN PR7000-1408 Multiplicom N.V. [ a ] Galileïlaan 18, B-2845 Niel, Belgium [ w ] www.agilent.com [ e ] [email protected] [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16