Hamamatsu A10035-90 Optical components for optical measurement Datasheet

OPTO OPTICAL BLOCKS
MECHANICS Optical Components for Optical Measurement
OVERVIEW
FEATURES
The optical blocks are compact units that contain components such
as bandpass filters and dichroic mirrors. They are designed for lowlight-level measurements that use PMT modules and high-sensitivity
cameras. Because their optical components are placed precisely
through the use of high-precision machining, the blocks shield light
superbly. Also, you can use the separate blocks in different combinations by removing and attaching them with thumbscrews.
●Easy to Attach / Detach
●No Alignment Required
●Superb Light-shielding Effect
●Makes Low-Light-Level
Measurement Easy
COMPARISON WITH BASIC OPTICAL DEVICES
Mechanics
Integration
Holder
Stage
Optics
Filter
Mirror
Lens
Light shield
Darkroom
environment
Detector
PMT
Photodiode
Camera
LINE UP
Photo
2
Product Name / Type No.
Over View
Page
Adapter Block
A10030 Series
The A10030 series is an adapter block for connecting a PMT module to a Vgroove type optical block.
4
C-Mount
Adapter Block
A10039
This block connects a device having C-mount to V-groove type optical
blocks. The connection angle for the device and block is adjustable.
4
Fiber Adapter Block
A10037 Series
Optical fiber cables with FC or SMA connectors can be connected to the
blocks in the A10037 series.
The lens assembled in the block collimates the light spread from the optical fiber.
5
Beam Expander Block
A10031
This optical block widens the diameter of a collimated beam of light to 2.5
times its original size or narrows the diameter to 1/2.5 times its original size.
5
Joint Block
A10038 Series
The A10038 series is a joint block for connecting one optical block to
another. The joint is available in two types: MM (male-male) type and FF
(female-female) type.
6
Cuvette Holder Block
A11971
This holder block is a small dark box specifically designed for cuvettes with
an optical path length of 10 mm, and allows easy measurement of samples
in combination with various optical blocks.
6
Shutter Block
A10036
The A10036 is a manual shutter block with a plate that is opened and
closed by sliding it left and right. Light is sufficiently blocked so there will be
no problem even at photon counting levels.
7
ND Filter Block
A10032 Series
A neutral density (ND) filter is included in this optical block to attenuate light
levels.
7
Filter Block
A10033 Series
This optical block contains a filter that passes only particular wavelengths.
Bandpass filters and long-pass filters are available.
8
Interchangeable
Filter Block
A10033-90
The A10033-90 is a holder block for assembling a commercially available
15 mm diameter filter.
9
Dichroic Block
A10034 Series
This block contains a dichroic mirror that reflects particular wavelengths but
passes other wavelengths. A total of six products are available.
10
Beam Splitter Block
A10035
This optical block uses a cubic type half-mirror. Incident light is split into
reflected and transmitted beams at a ratio of 1 to 1.
11
Beam Splitter
Holder Block
A10035-90
The A10035-90 is a holder block designed to hold a 15 mm cubic type
beam splitter.
11
Stage Block
A10858
This is a dark box equipped with 2 micrometers for positionings. It allows
light measurements even under room lighting by externally connecting various types of optical blocks.
12, 13
Beam Aligner Block
A10760
This optical block is for using an infinity-corrected objective lens to guide
laser light into an optical fiber with an attached FC connector or to collimate
and transmit the beam from a fiber-optic cable.
14
Tube Lens Block
A10859 Series
This block contains a focusing lens with a focal distance of 100 mm and is
used for an infinity-corrected objective lens. Aberration is reduced due to
the use of an achromatic lens.
15
Pinhole Holder Block
A11027
This is a feed screw type holder block that can hold a commercially available,
mount type pinhole and adjust its position in the XY direction. Light passing
through the pinhole is collimated by the lens installed in the block and is output.
16
C-Mount Interchangeable
Filter Block
A11213 Series
The A11213 series is a C-mount connection block for installing a commercially available 25 mm diameter filter or lens. This block allows angle adjustment
and so is useful as a spacer or adapter that connects between C-mounts.
17
Photo
Product Name / Type No.
Over View
Page
C-Mount Ring
A9865
This ring is for connecting a PMT module to a optical block and an equipment with C-mount.
17
C-Mount Interchangeable
Dichroic Block
A11214
The A11214 is a C-mount connection block for installing a dichroic mirror for
use at a 45° angle of incidence.
18
Polarizer Holder Block
A11026
This is a holder block designed for a polarizer.
Polarizing filters and waveplates are polarizing elements whose polarization
characteristics change depending on direction and angle.
19
Parts Set
A10455-02
This maintenance kit includes a set of screws and O-rings needed to assemble optical blocks.
19
CONNECTION EXAMPLES
Page
●Connection Example Using Bifurcated Optical Fiber ......................................................................................................................... 20
●Connection Example Using the Beam Expander Block ..................................................................................................................... 20
●Connecting to a Microscope ............................................................................................................................................................... 20
●Fluorescence Detection (Confocal) .................................................................................................................................................... 21
●Connection Example Using an Objective Lens and the Tube Lens Block ..................................................................................................... 21
●Imaging System Connection Example ............................................................................................................................................... 22
●Emission Detection ............................................................................................................................................................................. 23
●Example of Connection to a Mini-Spectrometer ................................................................................................................................. 23
●Simultaneous Measurement with a PMT Module and an Imaging Camera ..................................................................................................... 24
●Fluorescence Dye and Connecting Examples ..................................................................................................................................... 25
COMPATIBLE PMT MODULE
Screw connection with PMT module
Optical block with
V-groove connector
Optical block with C-mounts
Optical block with
V-groove connector
Optical block with
V-groove connector
Optical block with C-mounts
Adapter Block
A10030-01
H10720/H10721
H10682
H10722/H10723
H11890
H11526
C-Mount Ring
A9865
H10706/H12056
H10799
(built-in photodiode)
H7826/H7827/H7828
Adapter Block
A10030-02
C-mount connection with PMT module
H7421/H7422
C-Mount Adapter Block
A10039
C-mount adapter plate*
H11461/H11462/H8259
* In preparation for use with optical blocks, a C-mount adapter plate must be attached to the PMT module.
Please notify us of your need for this adapter when ordering.
3
BLOCK CHARACTERISTICS
●Adapter Block A10030-01, -02
The A10030-01 is an adapter block for connecting a PMT module to
each block. The A10030-01 adapter block has M4 and M3 screw holes
for securing to an optical bench or optical stage. A mini-post can also
be attached to the M4 screw holes.
Applicable mini-post: OptoSigma Model 155-0XXX
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
A10030-01
8 mm
Approx. 18 g
A10030-02
12 mm*
* When connected to other optical blocks, their effective optical path diameters
take precedence.
Dimensional Outlines (Unit: mm)
A10030-01
26.0±0.1
10.8±0.1
15.0±0.1
15.0±0.1
2-M3 DEPTH 3
(MOUNTING THREADED HOLE)
M4 DEPTH 5
0.5 (MOUNTING THREADED HOLE)
9
H10720/H10721/H10722/H10723/H11706
A10030-01 H12056/H10682/H11890/H11526
H10799 (built-in photodiode)
A10030-02 H7826/H7827/H7828
22.0±0.1
38.2±1.0
COMPATIBLE PMT MODULES
GASKET
4-M2
(THUMB SCREW
FOR PMT MODULE)
9
NOTE: Avoid frequently attaching and detaching from PMT
module.
8
4
TPMOA0050EA
A10030-02
27.0±0.1
18.0±0.1
10.5±0.1
15.5±0.1
GASKET
15.0±0.1
18.0±0.1
23.0±0.1
38.2±1.0
4-M2
(THUMB SCREW
FOR PMT MODULE)
8
2-M3 DEPTH 4
(MOUNTING THREADED HOLE)
M4 DEPTH 5
(MOUNTING THREADED HOLE)
0.5
4
TPMOA0085EA
●C-Mount Adapter Block A10039
One end of this adapter block has a C-mount (male threads) that connects to the H7421 series or H7422 series PMT module, while the other end couples to another optical block. This adapter block also connects to a commercially available device with a C-mount.
This adapter block can also be connected to commercially sold Cmount products.
SPECIFICATIONS
Type No.
A10039
Weight
Approx. 27 g
Effective Beam Size
8 mm
* Supplied: C-mount double female thread ring.
4
2. Insert the C-mount ring
into the C-mount of the
PMT module and turn
the adapter block body
clockwise until it stops.
3. Turn the adapter block
body counterclockwise
so that it is positioned
at the desired angle
and then tighten the
four screws.
C-MOUNT (MALE)
32
22.0±0.1
1. Loosen the four screws
on the adapter block
and make sure the Cmount ring is separated
about 1 mm from the
adapter block body.
4
22.0±0.1
How to connect a C-mount adapter block
38.2±1.0
Dimensional Outlines (Unit: mm)
16.5±0.2
3.5±0.1
TACCA0287EA
●Fiber Adapter Block A10037 Series
Optical fiber cables with FC or SMA connectors can be connected to
the blocks in the A10037 series.
The lens assembled in the block collimates the light spread from the
optical fiber.
The A10037/A10037-01 is suitable for use in the visible region (400 nm
to 700 nm) and the A10037-10/A10037-11 for use in the UV region
(200 nm to 400 nm).
SPECIFICATIONS
▲A10037-01
Dimensional Outlines (Unit: mm)
Transmittance (Typical)
22.0±0.1
TACCB0094EB
(8)
60
( 16)
80
TRANSMITTANCE (%)
7.0±0.1
22.0±0.1
100
Weight
Recommended Wavelength Connecter Focal Length
FC
Visible
SMA
10 mm
Approx. 17 g
FC
UV
SMA
4.6±0.1
▲A10037
Type No.
A10037
A10037-01
A10037-10
A10037-11
A10037/-10
: L=9.0
A10037-01/-11 : L=13.5
L
40
When fiber core diameter is 100 µm and NA is 0.2
Distance from Fiber Output End
Beam Diameter
100 mm
Max. 4.9 mm
150 mm
Max. 5.4 mm
20
A10037 / A10037-01
A10037-10 / A10037-11
0
200
300
400
500
600
700
* In the case of the SMA connector type, the beam diameter
depends on the position of the connected fiber cable end.
The above values are measured when the fiber cable end is
at the same position as the FC connector type.
800
WAVELENGTH (nm)
TACCA0270EB
●Beam Expander Block A10031
This optical block widens the diameter of a collimated beam of light to 2.5
times its original size or narrows the diameter to 1/2.5 times its original
size. This block has a C-mount thread for connecting to another device.
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
A10031
8 mm
Approx. 43 g
* Supplied: C-mount double male thread ring.
** Designed for wavelength from 400 nm to 700 nm.
36.0±0.2
TACCB0095EB
( 16)
100
9.0±0.1
Transmittance (Typical)
30.0±0.2
Input of parallel light
collimated to a
20 mm or less
Dimensional Outlines (Unit: mm)
C-MOUNT (FEMALE)
20
400
500
600
700
800
44.4±0.3
8
8
TACCA0271EA
( 16)
Incident light conditions
40
0
300
44.4±0.3
8
(PARALLEL)
60
20
(PARALLEL)
TRANSMITTANCE (%)
80
WAVELENGTH (nm)
TACCC0127EA
5
BLOCK CHARACTERISTICS
●Joint Block A10038 Series
The A10038 series is a joint block for connecting one optical block to
another. The joint is available in two types: MM (male-male) type and FF
(female-female) type. Interposing the MM block (A10038-01) and/or FF
block (A10038-02) between optical blocks allows various connections.
SPECIFICATIONS
Type No.
A10038-01
A10038-02
Effective Beam Size
8 mm
8 mm
Weight
Approx. 10 g
Approx. 25 g
Dimensional Outlines (Unit: mm)
5.0±0.1
16
22.0±0.1
▲A10038-01
9.0±0.1
A10038-01
22.0±0.1
(8)
(8)
22.0±0.1
19.0±0.2
TACCA0284EA
38.2±1.0
22.0±0.1
A10038-02
▲A10038-02
TACCA0285EA
●Cuvette Holder Block A11971
The A11971 cuvette holder block is a small dark box specifically
designed for cuvettes with an optical path length of 10 mm. It
allows easy measurement of samples in combination with other
optical blocks. The base can be removed as needed.
NOTE: This cuvette holder block is very compact. If it falls over, the sample
may leak from the cuvette, so we recommend securing the body of
the holder block and / or using a capped cuvette.
SPECIFICATIONS
Applicable Cuvette Size
Effective
Weight
Beam Size Optical Path Length Dimensional Outline
12.5 mm ×
Approx. 206 g
8 mm
10 mm
56 mm (H) (including the base)
Type No.
CUVETTE HOLDER BLOCK
Connection Example: Absorbance and Side
Fluorescence Detection Using Optical Fiber
A11971
Dimensional Outlines (Unit: mm)
(25)
PMT MODULE
DASHED LINE CUVETTE
28.1
38.2 ± 1.0
ADAPTER BLOCK A10030-01
19.5
BLOCK CAP
(10)
21
25.0 ± 0.1
PHOTOSENSOR MODULE
H10799-xxx
18.0 ± 0.1
18.0 ± 0.1
CUVETTE HOLDER BLOCK + CUVETTE
NOTE: Cuvette is sold separately.
8.1
2
6
8.1
20
16
14 ± 1
BASE MOUNTING SURFACE
50.0 ± 0.1
CUVETTE HOLDER
BLOCK
A11971
A'
32
15.0 ± 0.1
FIBER ADAPTER
BLOCK
A10037-xx
85
BASE
(REMOVABLE)
A
25.0 ± 0.1
2 × 4.5
(FOR HOLDING
THE BASE IN
PLACE)
FILTER BLOCK
A10033-xx
43.0 ± 0.2
22
21
56
22
8.1
BLOCK CAP
60
30
CROSS SECTION A–A'
(WHEN BLOCK CAP IS REMOVED)
M3
BOLTS WITH HEXAGONAL HOLES
FOR ATTACHING THE BASE
TPMOA0086EA
●Shutter Block A10036
The A10036 is a manual shutter block with a plate that is opened and
closed by sliding it left and right. Light is sufficiently blocked so there
will be no problem even at photon counting levels.
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
A10036
8 mm
Approx. 48 g
22.0±0.1
-0.02
12.0±0.1
16.00 -0.05
24.0±0.1
9.0±0.1
NOTE: If you feel the shutter does not open and close
smoothly, we recommend applying small amount of
an optical grease to the plate.
49.0±0.2
11.0±0.1
21.0±0.1
22.0±0.1
38.2±1.0
Dimensional Outlines (Unit: mm)
2
16.0±0.1
30.0±0.1
(8)
TACCA0288EA
●ND Filter Block A10032 Series
A neutral density (ND) filter is included in this optical block to attenuate
light levels.
SPECIFICATIONS
Type No. Built-in Filter Transmittance Effective Beam Size
Weight
A10032-10
10 %
ND Filter
8 mm
Approx. 26 g
1%
A10032-11
* Light transmittance is the average transmittance at wavelengths from 400 nm to 700 nm.
Dimensional Outlines (Unit: mm)
2
9.0±0.1
20.0±0.1 (8)
Transmittance (Typical)
80
TACCB0087EA
A10032-10
( 16)
40
22.0±0.1
38.2±1.0
TRANSMITTANCE (%)
22.0±0.1
60
A10032-11
20
TACCA0272EA
0
300
400
500
600
700
WAVELENGTH (nm)
7
BLOCK CHARACTERISTICS
●Filter Block A10033 Series
This optical block contains a filter that passes only particular
wavelengths. Bandpass filters and long-pass filters are available.
If you would like to use a filter with specifications other than those
described below, purchase the A10033-90 interchangeable filter block
(p. 9), which does not come with a preset filter. The A11213 C-mount
interchangeable filter block series (p. 17) is also available. Filters with
25 mm diameters can be installed in these blocks.
SPECIFICATIONS
Type No.
A10033-01
A10033-02
A10033-03
A10033-04
A10033-05
A10033-06
Built-in Filter
Bandpass Filter
Central Wavelength
530 nm ± 4 nm
610 nm ± 4 nm
630 nm ± 5 nm
460 nm ± 4 nm
575 nm ± 4 nm
700 nm ± 5 nm
FWHM
40 nm ± 8 nm
40 nm ± 8 nm
60 nm ± 10 nm
60 nm ± 8 nm
30 nm ± 8 nm
60 nm ± 10 nm
Effective Beam Size
8 mm
Weight
Approx. 25 g
* Stopband: Below 0.01 % at 300 nm to 950 nm (except transmission bands)
Type No.
A10033-61
A10033-62
A10033-63
A10033-71
Built-in Filter
Longpass Filter
Cut-on Wavelength Effective Beam Size
Weight
390 nm
500 nm
8 mm
Approx. 25 g
600 nm
510 nm
Transmittance [Bandpass filter] (Typical)
A10033-01
A10033-02
TACCB0077EA
100
90
80
80
70
60
50
40
30
TRANSMITTANCE (%)
90
70
60
50
40
30
60
50
40
30
20
20
10
10
10
400
500
600
700
0
300
800
400
600
700
0
300
800
100
100
80
50
40
30
TRANSMITTANCE (%)
90
80
TRANSMITTANCE (%)
90
80
60
70
60
50
40
30
10
WAVELENGTH (nm)
800
400
800
30
20
700
700
40
10
600
500
600
WAVELENGTH (nm)
700
800
TACCB0082EA
50
10
500
800
60
20
400
700
70
20
0
300
600
A10033-06
TACCB0081EA
90
70
500
WAVELENGTH (nm)
A10033-05
TACCB0080EA
0
300
400
WAVELENGTH (nm)
A10033-04
100
500
TACCB0079EA
70
20
WAVELENGTH (nm)
TRANSMITTANCE (%)
100
80
0
300
8
A10033-03
TACCB0078EA
90
TRANSMITTANCE (%)
TRANSMITTANCE (%)
100
0
300
400
500
600
WAVELENGTH (nm)
Transmittance [Longpass filter] (Typical)
A10033-61, A10033-62, A10033-63 (Colored glass filter)
100
A10033-71
TACCB0083EA
100
90
90
80
80
A10033-61
A10033-62
A10033-63
TRANSMITTANCE (%)
TRANSMITTANCE (%)
TPMOB0214EA
70
60
50
40
30
70
60
50
40
30
20
20
10
10
0
300
400
500
600
700
0
300
800
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
* Transmittance characteristics are typical examples and
may slightly vary depending on production lots.
* Dimensional outlines are the same as the A10033-90.
●Interchangeable Filter Block A10033-90
How to install an optical filter
The A10033-90 is a filter holder block that allows you to insert an
optical filter according to your application. The filter is held by the
spacer ring and the retaining ring, and can be easily exchanged by
using the accessory tool.
SPECIFICATIONS
1. Loosen the thumbscrews.
2. Insert, in order, the optical filter,
spacer ring and retaining ring.
Type No.
Effective
Beam Size
A10033-90
8 mm
Conpatible Filter Size
Weight
Diameter: 12.7 mm (1/2 inch) to 15 mm (Max.)
Approx.
(recommended: 15 mm)
24 g
Thickness: 1.5 mm to 5 mm
Dimensional Outlines (Unit: mm)
2
NOTE: • Filters for this block must be purchased separately.
• Do not install optical filters in the wrong direction because they have the front side and back side for an
light beam. Always insert the O-ring for blocking light
after installing an optical filter.
• When installing an optical filter, be careful to keep it
clean and to avoid getting fingerprints on it.
9.0±0.1
20.0±0.1 (8)
4. Install the O-ring in place.
22.0±0.1
38.2±1.0
22.0±0.1
( 16)
3. Tighten the retaining ring with
the tightening tool.
TACCA0272EA
Connection Example: Fiber-Optic Single Wavelength Detection
The lens inside the A10037 fiber adapter block collimates the beam of the light signal emitted from the optical fiber. The A10033 filter
block only allows a specific wavelength to pass. When dimming the beam, use ND filter block A10032.
FILTER BLOCK
A10033-xx
FILTER BLOCK
A10033-xx
PMT MODULE
ND FILTER BLOCK
A10032-xx
PMT MODULE
ADAPTER BLOCK A10030-01
FIBER ADAPTER BLOCK A10037-xx
FIBER ADAPTER BLOCK
A10037-xx
ADAPTER BLOCK
A10030-01
TPMOC0211EA
9
BLOCK CHARACTERISTICS
●Dichroic Block A10034 Series
This optical block contains a dichroic mirror that reflects particular
wavelengths but transmits other wavelengths.
If you would like to use a mirror with specifications other than those
described below, purchase the A11214 C-mount interchangeable
dichroic block (p. 18), which does not come with a preset mirror.
SPECIFICATIONS
Built-in Dichroic
Mirror
DM505
DM565
DM600
DM660
DM430
DM455
Type No.
A10034-01
A10034-02
A10034-03
A10034-04
A10034-05
A10034-06
NOTE: • Ultraviolet light shorter than 380 nm is absorbed by the
coating materials deposited on the dichroic mirror, so the
reflectance and transmittance significantly decrease.
• For the reflected light, use the wavelength range where
light is reflected more than 95 % (see the figures below).
Effective
Beam Size
Weight
8 mm
Approx. 57 g
Transmittance (Typical)
A10034-01
90
A10034-02
TACCB0084EA
100
Reflectance 95 % or more
at 445 nm to 480 nm
TRANSMITTANCE (%)
TRANSMITTANCE (%)
60
50
40
30
60
50
40
30
70
60
50
40
30
20
20
10
10
10
500
600
700
0
300
800
400
WAVELENGTH (nm)
600
700
0
300
800
100
Reflectance 95 % or more
at 460 nm to 625 nm
100
Reflectance 95 % or more
at 380 nm to 405 nm
50
40
30
70
60
50
40
30
50
40
30
20
10
10
10
500
600
WAVELENGTH (nm)
700
800
400
500
600
700
Reflectance 95 % or more
at 385 nm to 420 nm
60
20
400
800
70
20
0
300
700
TACCB0097EA
80
TRANSMITTANCE (%)
TRANSMITTANCE (%)
60
600
90
80
70
500
A10034-06
TACCB0096EA
90
80
0
300
400
WAVELENGTH (nm)
A10034-05
TACCB0093EA
90
500
WAVELENGTH (nm)
A10034-04
100
Reflectance 95 % or more
at 540 nm to 575 nm
80
70
20
400
TACCB0086EA
90
80
70
0
300
TRANSMITTANCE (%)
100
Reflectance 95 % or more
at 510 nm to 545 nm
90
80
A10034-03
TACCB0085EA
TRANSMITTANCE (%)
100
800
0
300
400
WAVELENGTH (nm)
500
600
700
800
WAVELENGTH (nm)
* Dimensional outlines are the same as the A10035 (see the dimensional outlines on the next page).
Connection Example: Fiber-Opic Dual Wavelength Detection
PMT MODULE
The lens inside the A10037 fiber adapter block collimates the beam
of the light signal emitted from the optical fiber. The dichroic mirror
inside the A10034 dichroic block reflects light below a certain
wavelength and passes light above that wavelength. Finally, each of
the two A10033 filter blocks isolates a specific wavelength so that the
signal levels of the two separate wavelengths can be measured.
FILTER BLOCK
A10033-xx
ADAPTER BLOCK
A10030-01
FIBER ADAPTER BLOCK
A10037-xx
10
ADAPTER BLOCK
A10030-01
DICHROIC BLOCK
A10034-xx
●Beam Splitter Block A10035
This optical block uses a cubic type half-mirror. Incident light is split into
reflected and transmitted beams at a ratio of 1 to 1.
SPECIFICATIONS
Type No.
A10035
Effective Beam Size
8 mm
Weight
Approx. 63 g
* Designed for wavelength from 400 nm to 700 nm.
NOTE: Light level is cut to approximately one third due to light
absorption.
2
22.0±0.1
26.0±0.5
11.0±0.1
Dimensional Outlines (Unit: mm)
Transmittance (Typical)
( 16)
TACCB0089EC
P
( 16)
60
38.2±1.0
30.0±0.2
80
AVERAGE
13.0±0.1
TRANSMITTANCE (%)
9.0±0.1
9.0±0.1
(8)
100
40
22.0±0.1
37.0±0.2
20
(8)
TACCA0273EA
S
0
400
450
500
550
600
650
700
WAVELENGTH (nm)
●Beam Splitter Holder Block A10035-90
The A10035-90 is a holder block designed to hold a 15 mm cubic type
beam splitter.
SPECIFICATIONS
Type No.
Effective
Beam Size
A10035-90
8 mm
Applicable Beam Splitter Size
Weight
15 mm × 15 mm × 15 mm cubic type Approx. 55 g
* Supplied: Hex wrench
Dimensional Outlines (Unit: mm)
2
26.0
NOTE: • The beam splitter is not included.
• Be careful to install the beam splitter so that it faces
in the correct direction.
• When installing a beam splitter, be careful to keep it
clean and to avoid getting fingerprints on it.
DASHED LINE
APPLICABLE BEAM SPLITTER SIZE
15 × 15 × 15
( 16)
(39)
17±0.1
(8)
4-M3 DEPTH 6
(FOR HOLDING THE
BLOCK IN PLACE)
22.0
20.0±0.1
9.0±0.1
( 16)
8
22.0±0.1
13.0
22.0
38.2±1.0
17±0.1
9.0±0.1
15.0±0.4
11.0±0.1
(26)
20.0±0.1
BOTTOM VIEW
TPMOA0068EA
11
BLOCK CHARACTERISTICS
●Stage Block A10858
The stage block is for using an infinity-corrected objective lens in object
image generation and fluorescence detection. It has two C-mount ports on
the top and bottom of its rear panel. You can use other optical blocks to
connect the stage block to a PMT module or ICCD camera (p. 27). Because
you can use the stage block cover to keep the measured area in darkness,
the stage block is useful even when detecting faint light. Furthermore, if you
remove the cover and illuminate the sample appropriately using an
illuminator, you can generate images by using the stage block with the
A10859-01 tube lens block, CCD cameras, and other devices.
SPECIFICATIONS
Type No.
A10858
Travels
X-Axis
Z-Axis
Compatible
Weight
Objective Lens
Infinity-corrected Objective lens
Approx.
±6.5 mm ±3 mm 2; C-mount
M20.32 × P0.706
4 kg
Parfocal distance*: 45 mm
Port
* For information about the parfocal distance, see p. 14.
Dimensional Outlines (Unit: mm)
50
140
110
(38)
160
81
Directions that you can
move the workplate in
using a micrometer.
245
135
2-C-MOUNT
Direction of beam
movement caused
by beam steering
mirror assembly
adjustment
Beam steering
mirror assembly
setup screws
Accesories
TPMOA0049EA
Workplate and Beam Movement
From outside of the stage block, you can use a micrometer to move the
workplate horizontally along the X axis or vertically along the Z axis.
You can also use the three setup screws in the beam steering mirror
assembly to change the internal mirror angle and adjust the horizontal
Y axis.
Dimensional Outlines (Unit: mm)
Stage Block Rack A11058
204
250
(127)
(38)
(ADJUSTABLE
RANGE 25 to 38)
4-M6
204
Sizes of the included spacers
12
HEIGHT-ADJUSTMENT BOLT
8
Spacer 1
90 mm × 90 mm, Thickness: 10 mm
2
Spacer 2
60 mm × 60 mm, Thickness: 18 mm
1
Spacer 3
60 mm × 60 mm, Thickness: 25 mm
1
The spacer sizes have been designed according to their uses.
(173)
(ADJUSTABLE RANGE
160 to 173)
8
250
TPMOA0066EA
Part Names and Features
M6 Mounting Hole
M6-thread mounting hole. You
can use a mounting post or an
angle mount for illumination.
C-Mount Port
There are two C-mount ports at
the top and bottom of the stage
block. You can connect the
stage block to other optical
blocks through blocks such
as the A11213 C-mount interchangeable filter block.
Workplate
The stage block comes with three different
workplates:
A workplate with a 36 mm diameter hole. With this workplate,
you can put the sample on a
slide and observe its reflectedlight or transmitted-light image
or measure its fluorescence.
This workplate is specially designed for use with the
A10657-01 microwell slide
(sold separately).
A workplate that you can
place an object on to observe
its reflected-light image in an
upright configuration or to excite it from above and measure its fluorescence.
* Please purchase illuminators from a
company that manufactures them.
Micrometer
The micrometer enables you
to perform precise horizontal
(X-axis) and vertical (Z-axis)
alignment and to accurately
reproduce prior positionings.
NOTE: Objective lens is not included.
Spacer
Objective Lens Mount A10921
A 20 mm spacer is installed
between the workplate and
the XZ axis stage. Removing this spacer will give you
20 mm of extra space,
which is useful when observing relatively tall objects
in an upright configuration.
You can move the workplate along the X and Y axes by attaching an X-axis
stage (SURUGA SEIKI
B11-40C) in place of the
spacer.*
This is a mount for an objective lens. You can mount and remove
the objective lens mount using the left and right screws. You can
place the objective lens mount at the top of the stage mount for
upright configurations, and at the bottom for inverted configurations. The A10858 comes with one objective lens mount.
45 mm
Parfocal distance
45 mm
* Please direct inquiries about
the X-axis stage to SURUGA
SEIKI.
Micrometer height
adjustment of
± 3 mm
NOTE: An additional A10921 objective lens mount (sold
separately) is attached in the above example.
Stage clips are not included.
Accesories
Objective Lens Mount A10921
Microwell Slide A10657-01
* There is a separate catalog for microwell slides.
Please refer to that catalog for more information.
13
BLOCK CHARACTERISTICS
●Beam Aligner Block A10760
This optical block is for using an infinity-corrected objective lens to
guide laser light into an optical fiber with an attached FC connector or
to collimate and transmit the beam from a fiber-optic cable.
The block has two micrometers for positioning the optical fiber along
the X and Y axes and an additional micrometer at the bottom for fine
adjustments along the Z axis.
This block has a cover around the objective lens and an additional
cover for the entire block that further increases its light shielding
capability.
SPECIFICATIONS
NOTE: Objective lens and fiber-optic cable are not included.
Type No.
Optical Fiber
Travels
XY-Axis Z-Axis Connector
A10760
±1 mm
±3 mm
Compatible
Weight
Objective Lens
Infinity-corrected Objective lens
Approx.
M20.32 × P0.706
420 g
Parfocal distance: 45 mm
FC
* Supplied: Hex wrench
USING EXAMPLE 1: FOCUSING
BEAM ALIGNER BLOCK
A10760
Dimensional Outlines (Unit: mm)
OBJECTIVE LENS
SCREW
OPTICAL FIBER
INFINITY-CORRECTED
OBJECTIVE LENS
C-MOUNT (FEMALE)
TPMOC0209EB
69
USING EXAMPLE 2: COLLIMATOR
43
BEAM ALIGNER BLOCK
A10760
OPTICAL FIBER
INFINITY-CORRECTED
OBJECTIVE LENS
70
52
TPMOC0210EA
TPMOA0051EA
Technical Guide
Pupil Diameter and Parfocal Distance
PUPIL DIAMETER
OBJECTIVE
MOUNTING POSITION
PARFOCAL DISTANCE
1. Remove the two cover screws using a
Phillips-head screwdriver, and then
remove the cover.
2. Use the hexagonal wrench that comes
with the block to remove the two plate
screws, and then remove the plate.
Objective lens
Y-axis
adjustment
screw
SAMPLE SURFACE
TPMOC0223EA
Pupil Diameter
The diameter of the beam that is emitted from the pupil
of an objective lens with a focal length f and a numerical
aperture NA is given by:
Pupil diameter = 2 × f × NA
Parfocal Distance
The parfocal distance is the distance from the objective
mounting position of the objective lens to the sample at
which the sample is in-focus. The A10760 is designed to
be used with an objective lens whose parfocal distance
is 45 mm.
14
X-axis
adjustment screw
3. Attach an objective lens to the plate's
objective lens screw hole (M20.32
P0.706).
Z-axis
adjustment screw
4. Reassemble the block by performing
steps 1 and 2 in reverse, and then
connect the block to other devices.
You can align the optical fiber by using
the X-, Y-, and Z-axis adjustment
screws. Only turn the Z-axis adjustment
screw after you have loosened the
screws in step 1.
●Tube Lens Block A10859 Series
▲A10859
▲A10859-01
Transmittance (Typical)
100
These are video lens blocks for use with the infinity-corrected objective
lenses. It has a tube lens with a focal length of 100 mm to form an
image. One side has a male C-mount thread, and the other side has a
female C-mount thread. You can attach a device such as an ICCD or
CCD camera to the side with the male thread.
You can use a C-mount double male thread ring to connect the side
with the female thread to the A10858 stage block.
The tube lens block reduces aberration through the use of an
achromatic lens.
TPMOB0251EA
TRANSMITTANCE (%)
80
60
40
SPECIFICATIONS
20
Type No.
0
300
400
500
600
700
800
900
Focal Length Effective Beam Size
A10859
15 mm
100 mm
A10859-01
24 mm
* Supplied: C-mount double male thread ring.
* Designed for wavelength from 400 nm to 700 nm.
1000
WAVELENGTH (nm)
Dimensional Outlines (Unit: mm)
A10859
A10859-01
C-MOUNT (FEMALE)
MANUAL
FOCUSING RING
CAMERA ANGLE
ADJUSTING SCREW*
C-MOUNT (FEMALE)
MANUAL
FOCUSING RING
CAMERA ANGLE
ADJUSTING SCREW*
C-MOUNT (MALE)
40
42
30
C-MOUNT (MALE)
32
Recommended
Weight
Sensor Size
1/3 inches or less Approx. 130 g
1/2 inches or less Approx. 205 g
IMAGE PLANE
4
IMAGE PLANE
4
93 (92-94)
4
17.526
4
96 (95-97)
TPMOA0052EA
17.526
* You can adjust the camera tilt by loosening this screw and turning the tube lens block.
TPMOA0053EA
Technical Guide
Imaging Lens
Distance Between the Imaging Lens and the Objective Lens
The light from the sample is collimated by the infinitycorrected objective lens before it enters the imaging lens.
The imaging lens focuses the image on the focal point.
Even though the beam of light that passes from the infinity-corrected
objective lens to the imaging lens is collimated, the distance between the
lenses must still be kept below a certain limit. This limit (L) can be
calculated from the specifications of the objective and imaging lenses and
the effective area of the sensor.
COLLIMATED
LIGHT BEAM
CAMERA
L (mm) =
INFINITY-CORRECTED
OBJECTIVE LENS
IMAGING LENS
TUBE LENS BLOCK
A10859 / A10859-01
f2(φ2 – φ1)
φ
φ1 = 2 × f1 × NA
TPMOC0224EA
Magnification
You can calculate the magnification from the imaging lens
focal length (100 mm) and the focal length of the
microscope's objective lens.
Imaging lens focal length
100
Magnification (M) =
=
Objective lens focal length Objective lens focal length
Example: When the objective lens focal length is 45 mm, the
magnification is approximately 2.2 times.
d=
φ
M
where φ1 is the objective lens's exit pupil
diameter (mm), f1 is the focal length (mm), NA
is the numeric aperture, φ2 is the imaging lens
effective diameter, f2 is the focal length (mm),
φ is the effective sensor area, and d is the
observation area.
To avoid vignetting when using the A10859 with objective lens (f1 = 18
mm, NA = 0.25) and a 1/3-inch video camera (φ = 6 mm), keep the
distance from the objective lens to the A10859 below 100 mm. At this
distance, placing a dichroic mirror between the objective lens and the
A10859 will not have much of an effect on the overall optical
characteristics.
15
BLOCK CHARACTERISTICS
●Pinhole Holder Block A11027
The A11027 is a holder block for mounting a pinhole. The screws can
be used to adjust the pinhole's position in the XY directions. The built-in
lens collimates the light that passes through the pinhole and converges
the light on a photodetector.
There is a base attached to the holder block through the M3 screw
holes in the bottom of the block. If necessary, this base can be removed and re-attached to the side of the block.
SPECIFICATIONS
Type No.
A11027
Diameter: 16 mm
Thickness:
1.58 mm to 1.6 mm
±1.2
0.25 mm
15 mm
Y-AXIS ADJUSTMENT SCREW
ADJUSTMENT: ±1.2
C-MOUNT ANGLE
ADJUSTMENT RING
2-M3 DEPTH 3
(FOR HOLDING THE
BLOCK IN PLACE)
50.0
(REFERENCE PLANE OF
THE PINHOLE MOUNT) 17.746
(27.526)
(ASSUMED PINHOLE PLANE) 17.526
10.0
PINHOLE MOUNTING
SCREW RING
M4 DEPTH 5
(FOR HOLDING THE
BLOCK IN PLACE)
40
50.0
4.6
( 16)
29.0
45.0
(25)
43.0
12.0
5.0
22.95
30.0
(18)
TRANSMITTANCE (%)
80
60
8.0
(0.22)
11.5±1.5
12.0
50.0
TPMOB0233EA
15.0±0.1
100
Approx. 180 g
(including
the base)
Dimensional Outlines (Unit: mm)
X-AXIS ADJUSTMENT
SCREW
ADJUSTMENT: ±1.2
Transmittance for the Collimation Lens (Typical)
Weight
* Supplied: Screw ring tool and hex wrench
11.5±1.5
NOTE: • The collimate status changes depending on the pinhole diameter and the wavelength. (The assumed
wavelength is between 400 nm and 700 nm.)
• The pinhole is not included. Use a pinhole that is installed in a mount. (Products by companies such as
SIGMA KOKI CO., LTD.; SURUGA SEIKI CO., LTD.;
and CVI Melles Griot can be used.)
• Be careful to install the pinhole so that it faces in the
correct direction.
• When installing a pinhole, be careful to keep it clean
and to avoid getting fingerprints on it.
Applicable Pinhole XY Axis Range Adjustment Focal
Mount Size
of Motion Screw Pitch Length
C-MOUNT
(FEMALE)
APPLICABLE PINHOLE MOUNT SIZE
DIAMETER: 16. THICKNESS: 1.6
2-M3
BOLTS WITH HEXAGONAL HOLES
FOR ATTACHING THE BASE
BASE (REMOVABLE)
2- 4.5
(FOR HOLDING THE BASE IN PLACE)
60.0
BASE MOUNTING SURFACE
TACCA0070EA
20
Extended Example of Microscope
0
300
400
500
600
WAVELENGTH (nm)
700
800
Connecting the A11027 pinhole holder block to the C-mount port of a
microscope configures a confocal optical system. Light passing
through the pinhole is collimated by the lens in the block and is efficiently guided to the PMT module.
FILTER BLOCK A10033-xx
PMT MODULE
ADAPTER BLOCK
A10030-01
PINHOLE HOLDER BLOCK A11027
MICROSCOPE C-MOUNT PORT
MICROSCOPE
16
●C-Mount Interchangeable Filter Block A11213 Series
These C-mount threaded optical blocks are for installing commercial
optical filters and lenses with 25 mm diameters. You can use it with the
A11214 C-mount interchangeable dichroic block to measure fluorescence or perform other tasks.
Because this block enables angle adjustment, it is also useful as a
spacer between C-mounts and as a connection adapter.
SPECIFICATIONS
Type No.
Effective
Beam Size
A11213
A11213-01
20 mm
Weight
Compatible Filter Size
Diameter: 24 mm to 26 mm (25 mm to 25.4 mm recommended) Approx. 29 g
Thickness: 5 mm (Max.) (2 mm to 5 mm recommended)
Approx. 30 g
▲Left: A11213, Right: A11213-01
Connection Example: Single Wavelength Detection
NOTE: • Filters and lenses for this block must be purchased
separately.
• When you install a filter that is designed to receive a
beam of light from a specific side, make sure that you
install the filter in the correct direction.
• When installing an optical filter or other device, be careful to keep it clean and to avoid getting fingerprints on it.
Only light at a specific wavelength passes through the optical filter
assembled in the block and is detected by the PMT module.
C-MOUNT RING A9865
Dimensional Outlines (Unit: mm)
PMT MODULE
A11213 (Male–Male Type)
C-MOUNT (MALE)
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213-xx
2 (DATUM PLANE OF FILTER)
Connection Example: Fiber-Optic Single Wavelength Detection
35
Light emitted and spread from the optical fiber cable is collimated by
the lens in the A10037 series fiber adapter block and is efficiently
guided to the PMT module.
4
20
4
TPMOA0054EA
C-MOUNT ADAPTER BLOCK
A10039
A11213-01 (Male–Female Type)
C-MOUNT (FEMALE)
2 (DATUM PLANE OF FILTER)
C-MOUNT (MALE)
PMT MODULE
35
C-MOUNT RING A9865
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213-01
FIBER ADAPTER BLOCK A10037-xx
5
20
4
TPMOA0055EA
●C-Mount Ring A9865
This ring is for connecting a PMT module to a optical block and an
equipment with C-mount.
SPECIFICATIONS
Type No.
A9865
Effective Beam Size
8 mm
Weight
Approx. 7 g
Dimensional Outlines (Unit: mm)
GASKET
C-MOUNT
9
30
4- 2.1
14
4- 3.2
COMPATIBLE PMT MODULES
H10720/H10721/H10722/H10723/H11706/H12056/H10682
H11890/H11526
H10799 (built-in photodiode)
14
5.0
7.0
0.5
TPMOA0056EA
17
BLOCK CHARACTERISTICS
●C-Mount Interchangeable Dichroic Block A11214
This C-mount threaded optical block is for installing a dichroic mirror for
use at a 45° angle of incidence. You can use typical dichroic mirrors
that are commercially available for use with microscopes.
SPECIFICATIONS
Type No.
Effective
Beam Size
A11214
20 mm
Weight
Compatible Dichroic Mirror Size
Approx.
25 mm to 26 mm (W) × 35 mm to 38 mm (H)
Thickness: 0.9 mm to 1.1 mm (1 mm recommended) 84 g
* Supplied: Hex wrench
18
Dimensional Outlines (Unit: mm)
18
NOTE: • Dichroic mirrors for this block must be purchased separately.
• Commercially sold dichroic mirrors usually have markings to distinguish their front and back sides. Be careful to install dichroic mirrors in the A11214 in the correct direction.
• When installing a dichroic mirror, be careful to keep it
clean and to avoid getting fingerprints on it.
C-MOUNT (FEMALE) DEPTH 5
20
20
The dichroic mirror assembled in the block reflects light
at wavelengths shorter than a specific wavelength and
transmits longer wavelength light. Furthermore, only
light at a specific wavelength passes through the optical
filter and is then detected by the PMT module.
Dual Wavelength Detection
PMT MODULE
PMT MODULE
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
C-MOUNT
RING A9865
C-MOUNT
RING A9865
C-MOUNT
INTERCHANGEABLE
DICHROIC BLOCK A11214
FIBER ADAPTER
BLOCK A10037-xx
C-MOUNT ADAPTER BLOCK A10039
Triple Wavelength Detection
PMT MODULE
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
C-MOUNT
RING A9865
C-MOUNT
INTERCHANGEABLE
DICHROIC BLOCK
A11214
C-MOUNT ADAPTER BLOCK A10039
FIBER ADAPTER BLOCK A10037-xx
18
18
38
18
Connection Example:
Fiber-Optic Dual / Triple Wavelength Detection
20
C-MOUNT (FEMALE) DEPTH 5
20
C-MOUNT (FEMALE) DEPTH 5
18
18
40
18
18
TPMOA0057EA
●Polarizer Holder Block A11026
Polarizing filters and waveplates are polarizing elements whose polarization characteristics change depending on direction and angle. The
A11026 is a simple, course-movement holder block that can be used to
adjust the direction and angle.
SPECIFICATIONS
Adjustable Angle
Range Scale
Applicable Polarizing
Element Size
Effective
Type No. Beam Size
Weight
5° 2
Diameter: 25 mm to 25.4 mm
90° 1
Approx. 55 g
Thickness: 1 mm to 8 mm
Intervals
1Can be rotated 90° or more by changing the angle adjustment screw position.
2Scale indicates adjustment intervals and does not indicate adjustable pitch.
Can be fixed at any desired angle within the adjustable range of 90°.
A11026
8 mm
* Supplied: Hex wrench
Dimensional Outlines (Unit: mm)
ANGLE SCALE: 5° INTERVALS
34.0
22.0
ANGLE
ADJUSTMENT
RANGE
37.0±0.3
DASHED LINE
APPLICABLE OPTICAL ELEMENT SIZE
DIAMETER: 25.4. THICKNESS: 1 to 8
(8)
22.0
22.0
( 16)
°)
(90
34.0
ANGLE
ADJUSTMENT
SCREW
9.0±0.1
38.2±1.0
(23±1)
NOTE: • The polarizing filter or waveplate is not included.
(Products by companies such as Edmund Optics Inc.
and SIGMA KOKI CO., LTD. can be used.)
• When installing a polarizing element, be careful to
keep it clean and to avoid getting fingerprints on it.
32
2
(REFERENCE PLANE OF
THE OPTICAL ELEMENT) 17
22.0
24
20.0±0.1
2-M3 DEPTH 3
(FOR HOLDING THE BLOCK IN PLACE)
TACCA0069EA
●Parts Set A10455-02
This maintenance kit includes a set of screws and O-rings needed to assemble optical blocks.
Parts
Hex wrench
Thumb screws
Filter remove tool
Screw-ring tool
O-rings ( 15 mm)
O-rings ( 26 mm)
O-rings ( 28 mm)
Screws M2 × 12
Screws M2 × 16
Hex socket setscrews M2 × 2
Hex socket setscrews M2 × 4
Hex socket setscrews M3 × 4
Hex socket bolts M2 × 8
Hex socket bolts M2.6 × 6
Hex socket setscrews M3 × 16
Washers
* The A10030 is not currently sold.
Qty.
Optical blocks using parts at left
1
A10030*, A10035-90, A10760, A10858, A10859, A11027, A11213, A11214, A11971
(5 pcs/set)
4
1
1
2
1
1
10
10
10
10
10
10
10
5
3
A10030-01, A10030-02, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971
A10033-90
A11027
A10030-01, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971
A10039
A11026
A10030-01, A10030-02
A10039
A10035-90
A10030*
A11213
A10030*, A10035-90, A11026
A11214
A11027
A11026
19
CONNECTION EXAMPLES
●Connection Example Using Bifurcated Optical Fiber
Light from a light source is injected into one arm of the bifurcated optical fiber to irradiate a sample or measurement surface.
Fluorescence emitted from the sample or light reflected from the measurement surface is collected by the same optical fiber and then
detected by the PMT module through the other arm of the bifurcated optical fiber.
FILTER BLOCK A10033-xx
ADAPTER BLOCK A10030-01
PMT MODULE
FIBER ADAPTER BLOCK A10037
BANDPASS
LED
FILTER
TPMOC0213EA
●Connection Example Using the Beam Expander Block
The A10031 beam expander block is designed to narrow a 20-mm beam of collimated light to a diameter of 8 mm, the size of the
PMT module's photocathode; however, the expander block's use is not just limited to collimated light, it can also be used to converge
divergent light. For example, if you use the beam expander block to receive the divergent light from an optical fiber, the signal level
will be higher than if you had just used a PMT module.
BEAM EXPANDER BLOCK A10031
ADAPTER BLOCK A10030-01
OPTICAL FIBER
PMT MODULE
DISTANCE L1
DISTANCE L2
<Measurement Conditions>
Light source: Green LED
Optical fiber: NA=0.5. Core diameter=0.98 mm
Detection efficiency (Distance: 50 mm. Detection efficiency measured without beam expander block is normalized to 100.)
DISTANCE (L1, L2)
When Beam Expander is Connected (L1)
When Beam Expander is not Connected (L2)
50 mm
80 %
100 %
90 mm
59 %
59 %
250 mm
28 %
13 %
350 mm
20 %
7%
TPMOC0214EA
●Connecting to a Microscope
The microscope port is designed so that the image is focused at 17.526 mm from the C-mount attachment location. When you
connect a C-mount adapter to a commercially sold 10-mm C-mount ring (male to female), the approximate location of the image
point is at the PMT module's photocathode (as shown in the figure on the left).
If the light converges to a diameter of 1 mm or less at the image point, do not match the location of the image point to the location of
the PMT module's photocathode; instead, expose the photocathode to a defocused light beam with a diameter of 1 mm or more.
MICROSCOPE
C-MOUNT RING (MALE-FEMALE)
C-MOUNT RING
A9865
17.526
PMT
MODULE
C-MOUNT INTERCHANGEABLE
FILTER BLOCK
A11213-01 (SPACER)
C-MOUNT RING
A9865
MICROSCOPE
17.526
PMT
MODULE
L*
7 10
L*
7
25
* L is the distance from the attached end of the PMT module to the photocathode.
20
TPMOC0216EA
●Fluorescence Detection (Confocal)
For detecting fluorescence, it is convenient to use a laser with an optical fiber output. If you intend to focus the laser up to the
diffraction limit, use single-mode optical fiber. The infinity-corrected objective lens inside the beam aligner block collimates the light
beam emitted from the optical fiber. In this situation, you can collect the light efficiently if you use an objective lens with an NA that is
larger than that of the optical fiber. You can calculate the beam diameter from the optical fiber NA or the objective lens NA, whichever
is smaller, and the focal length of the objective lens.
The laser is reflected by the dichroic mirror, passes through the objective lens in the stage block, and excites the sample. The excited
fluorescence passes through the objective lens, dichroic mirror, and tube lens block and is focused on the position of the pinhole that
is installed in the pinhole block. This creates the confocal optical system. The light that passes through the pinhole is collimated by
the installed lens and is injected into the PMT module.
OPTICAL FIBER (FC CONNECTOR)
BEAM ALIGNER BLOCK A10760
LASER
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213 (FOR THE CONNECTOR)
PINHOLE HOLDER BLOCK A11027
STAGE BLOCK
A10858
PMT MODULE
ADAPTER BLOCK A10030-01
FILTER BLOCK A10033-xx
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
(FOR THE CONNECTOR)
PINHOLE (COMMERCIALLY AVAILABLE)
TUBE LENS BLOCK A10859-01
C-MOUNT INTERCHANGEABLE
DICHROIC BLOCK A11214
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10859-01)
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
TPMOC0217EA
Technical Guide
The performance of the confocal optical system is determined by the size of the pinhole. The Airy disk (diameter) is used as the
reference. The following equation is used to calculate the Airy disk. The system magnification is determined by the focal length of the
observation-side objective lens and the focal length of the tube lens block.
λ
Airy disk (diameter) = 1.22 × M ×
NA
Focal length of the tube lens block
Focal length of the observation-side objective lens*
λ : Fluorescence wavelength
NA: NA of the observation-side objective lens
M: System magnification =
* If the objective lens focal distance on the observation side is unknown, please contact the lens manufacturer.
If spatial resolution is important, use a pinhole whose size is close to the size of the Airy disk. If you are only measuring fluorescence
intensity, make the pinhole larger than the Airy disk; this will lower the spatial resolution, but it will also increase the signal level.
●Connection Example Using an Objective Lens and the Tube Lens Block
An infinity-corrected objective lens is connected to the A10859 tube lens block with a microscope objective lens mount, which has
threads for a M20.32 × 0.706 mm objective lens and a C-mount male thread. Next, the tube lens block is connected to an imaging
camera, such as an ICCD camera. You can adjust the focus using the manual focusing ring on the tube lens block.
TUBE LENS BLOCK A10859
OBJECTIVE
LENS
ICCD
CAMERA
MICROSCOPE OBJECTIVE LENS MOUNT *
(COMMERCIALLY AVAILABLE)
* Example: Edmund Optics
Microscope objective lens mount
TPMOC0215EA
21
CONNECTION EXAMPLES
● Imaging System Connection Example
Coaxial Incident Illumination Method
Illuminate the sample with a fiber bundle connected to a xenon arc lamp. You can attach the fiber bundle easily by using a
commercially sold C-mount light guide mount. To excite the sample, put an excitation filter that matches its fluorescent dye into the
A11213-01 C-mount interchangeable filter block. If the light from the fiber bundle is not diffused enough, you can illuminate the
sample evenly by inserting a holographic or other type of diffuser.
The dichroic mirror reflects the excitation light. Then, the objective lens of the A10858 stage block focuses the light onto the sample,
which is excited by the light. The fluorescence emitted from the sample passes through the dichroic mirror and the fluorescence filter
and is focused onto the imaging camera by the lens inside the A10859-01 tube lens block.
LIGHT GUIDE A10014-35-0110
XENON LAMP
L9588-03
LIGHT GUIDE ADAPTER*
(COMMERCIALLY AVAILABLE)
EXCITATION FILTER
(COMMERCIALLY AVAILABLE)
C-MOUNT LIGHT GUIDE MOUNT*
(COMMERCIALLY AVAILABLE)
HOLOGRAPHIC DIFFUSER*
(COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213-01
FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE)
EM-CCD
or
ICCD CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
TUBE LENS BLOCK A10859-01
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR)
STAGE BLOCK A10858
* Example: Edmund Optics
•C-Thread Light Guide Mounting Adapter
•Fiber Optic Light Guide Adapter SX-6
•Holographic Diffuser: 25 mm Type
TPMOC0218EA
Transmitted Illumination Method
This is an imaging example that uses transmitted illumination. The image of the sample is focused onto an imaging camera, such as
a CCD camera, by an infinity-corrected objective lens and the A10859-01 tube lens block. A convenient way to illuminate the sample
is to use a commercially sold LED illuminator.
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10859-01)
CCD
CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
TUBE LENS BLOCK
A10859-01
SAMPLE
LED LIGHT
SOURCE
STAGE BLOCK A10858
TPMOC0219JA
22
●Emission Detection
Collimated light such as chemiluminescence and bioluminescence captured by an infinity-corrected lens is reduced to 1/2.5 by using
the A10031 beam expander and is guided to the PMT module with an effective diameter of 8 mm. A filter block is added when
detesting light at a specific wavelength.
SAMPLE
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10031)
BEAM EXPANDER BLOCK A10031
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
PMT MODULE
ADAPTER BLOCK A10030-01
STAGE BLOCK A10858
FILTER BLOCK A10033-xx
TPMOC0255EA
●Example of Connection to a Mini-Spectrometer
The laser beam emitted from the optical fiber is collimated by the infinity-corrected objective lens inside the A10760 beam aligner
block. The dichroic mirror inside the A11214 C-mount interchangeable dichroic mirror block reflects the collimated laser, which then
passes through the objective lens in the A10858 stage block and illuminates the sample. The fluorescence from the excited sample is
converged by the objective lens and then passes through the dichroic mirror inside the A11214. The fluorescence is then converged
further by the A10031 beam expander block. The lens inside the fiber adapter block converges the light into the optical fiber and
guides it to the mini-spectrometer, where it is measured. If you want to remove the excitation light, place the A10033 filter block
between the beam expander block and the A10037-01 fiber adapter block. The fiber adapter block does not have focusing
capabilities, so we recommend that you connect the fiber adapter block and the mini-spectrometer with an optical fiber whose core
diameter is at least 600 µm.
OPTICAL FIBER (FC CONNECTOR)
BEAM ALIGNER BLOCK A10760
LASER
OBJECTIVE LENS (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213 (FOR THE CONNECTOR)
STAGE BLOCK
A10858
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10031)
JOINT BLOCK
A10038-02
FIBER ADAPTER BLOCK
A10037-01
OPTICAL FIBER (SMA CONNECTOR)
(RECOMMENDED FIBER CORE
DIAMETER : 600 µm or more)
C-MOUNT INTERCHANGEABLE
DICHROIC BLOCK A11214
BEAM EXPANDER BLOCK A10031
MINI
SPECTOROMETER*
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
* There is a separate catalog for mini-spectrometers.
Please refer to that catalog for more information.
TPMOC0221EA
23
CONNECTION EXAMPLES
●Simultaneous Measurement with a PMT Module and an Imaging Camera
The following is an example of simultaneous measurement in which the two A10921 objective lens mounts are attached to the
A10858 stage block. The lower (inverted) part excites the sample using laser light and measures the fluorescence of the sample
using the PMT module. The upper (upright) part illuminates the observation area using a xenon arc lamp and uses an imaging
camera to observe the observation area and the fluorescence signal.
A plano-convex lens and a fluorescence filter are installed in front of the PMT module to isolate the fluorescence. The plano-convex
lens converges the beam of light. If this causes a problem in the fluorescence filter's wavelength shift, place the filter in front of the
plano-convex lens.
So that there is no interference with the PMT module's measurement, use a wavelength for the imaging camera's illumination that is
different from both the excitation wavelength and the fluorescence wavelength. For example, if you use a 488 nm laser, use a DM505
dichroic mirror for the PMT module and a 530/40 bandpass filter for the emission filter. On the imaging camera side, use a 600 nm
longpass filter for illumination and put a laser-blocking filter in front of the camera.
LIGHT GUIDE A10014-35-0110
XENON LAMP
L9588-03
LIGHT GUIDE ADAPTER*
(COMMERCIALLY AVAILABLE)
C-MOUNT LIGHT GUIDE MOUNT*
(COMMERCIALLY AVAILABLE)
600 nm LONGPASS FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01
TUBE LENS BLOCK A10859-01
STAGE BLOCK A10858
OBJECTIVE
LENS MOUNT
A10921
EM-CCD
or
ICCD CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
LASER-BLOCKING FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
C-MOUNT CUBE BEAMSPLITTER* (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR)
C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
C-MOUNT RING A9865
OBJECTIVE
LENS MOUNT
A10921
PMT MODULE
FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213-01
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
(FOR THE CONNECTOR)
PLANO-CONVEX LENS (COMMERCIALLY AVAILABLE)
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
BEAM ALIGNER BLOCK A10760
OPTICAL FIBER (FC CONNECTOR)
LASER
* Example: Edmund Optics
•C-Thread Light Guide Mounting Adapter
•Fiber Optic Light Guide Adapter
•C-Mounted Cube Beamsplitter
NOTE: The A10858 stage block comes with one A10921 objective lens mount.
To perform simultaneous measurement as shown above, you will need to purchase an additional A10921.
TPMOC0222EA
24
●Fluorescence Dye and Connecting Examples
1
●Transmittance
633 nm 532 nm
100
A10034-04
DM 660
TACCB0090EA
90
A10033-05
575/30 nm
OBJECTIVE
LENS
TRANSMITTANCE (%)
80
A10033-06
700/60 nm
Cy3™
Alexa® 546
Cy5™
Alexa® 647
70
60
50
40
30
20
10
SAMPLE
0
400
500
600
700
800
700
800
700
800
TACCC0128EA
WAVELENGTH (nm)
2
●Transmittance
100
TACCB0091EA
90
80
EXCITATION
LIGHT
405 nm
A10033-04
460/60 nm
TRANSMITTANCE (%)
A10034-01
505 nm
FLUORESCENCE
A10033-01
530/40 nm
70
60
50
40
30
20
10
CASY
FITC
DAPI
Hoechst
0
400
500
600
WAVELENGTH (nm)
TACCC0129EA
3
●Transmittance
100
TACCB0092EA
90
EXCITATION
LIGHT
488 nm
A10034-02
DM 565
A10033-01
530/40 nm
FITC
GFP
A10034-03
DM 600
A10033-05
575/30 nm
PE
CASY
A10034-04
DM 660
A10033-03
630/60 nm
Texas Red®
PI
80
A10033-06
700/60 nm
Cy5™
7AAD
TRANSMITTANCE (%)
FLUORESCENCE
A10033-62
500 nmLP
70
60
50
40
30
20
10
0
400
500
600
TACCC0130EA
WAVELENGTH (nm)
25
RELATED PRODUCTS
●Stabilized Light Sources For PMT L11494 Series
The majority of these devices are LED light sources that have been
developed for visual-sensitive compensation of PMTs and PMT
modules.
Because the amount of light generated by the LED is monitored and
controlled by a photodiode, these devices always generate stable light
of approximately 1 pW.
SPECIFICATIONS
L11494-430 L11494-470 L11494-525 L11494-660 Unit
Parameter
Peak Wavelength of
428
465
522
660
nm
Generated Light
FWHM
65
26
35
30
nm
Wavelength Distribution of Generated Light (Typical)
●L11494-430
●L11494-470
TACCB0114EA
1
1.2
0.6
0.4
0.2
TACCB0120EA
1
0.8
RELATIVE ENERGY
RELATIVE ENERGY
0.8
Life Characteristic
TACCB0115EA
RELATIVE VALUE
1
0.6
0.4
0.8
0.6
0.4
0.2
0.2
0
350
400
450
500
550
600
650
700
0
350
750
400
450
WAVELENGTH (nm)
550
600
650
700
0
750
0
12
6
WAVELENGTH (nm)
●L11494-525
1
500
●L11494-660
TACCB0116EA
1
18
24
30
TIME (h)
Temperature Characteristic
TACCB0119EA
2
TACCB0121EA
1.5
0.6
0.4
0.2
RELATIVE VALUE (%)
0.8
RELATIVE ENERGY
RELATIVE ENERGY
0.8
0.6
0.4
1
0.5
0
-0.5
-1
0.2
-1.5
0
350
400
450
500
550
600
650
700
750
0
350
400
450
WAVELENGTH (nm)
500
550
600
650
700
750
-2
0
5
10
15
WAVELENGTH (nm)
Connection Example
20
25
30
35
40
45
50
TEMPERATURE (°C)
Dimensional Outlines (Unit: mm)
24
STABILIZED LIGHT SOURCE
L11494-xxx
8.5
12
12
2-M2
14
JOINT BLOCK A10038-01
EMISSION AREA
7
ON
OFF
ON
(Low intensity)
(High intensity)
14
65
PMT MODULE
ADAPTER BLOCK
A10030-01
* There is a separate catalog for stabilized light sources.
Please refer to that catalog for more information.
26
SWITCH
BATTERY SR41
TACCA0311EA
RELATED PRODUCTS
●Photosensor Modules H10799 Series
The H10799 series modules are cable output modules with S13371010 series photodiodes and current-to-voltage conversion amplifiers.
You can connect it easily to optical blocks and use it as a photodetector.
SPECIFICATIONS
H10799 Series
Parameter
Suffix
-001
-002
-102
-101
Spectral Response
190 nm / 320 nm to 1100 nm (See spectral response)
Input Voltage
+5 V
Max. Input Voltage
+5.5 V
+4.5 V
Max. Output Signal Voltage
0.1 V/nA / DC to 100 Hz
Current-to-voltage
1 V/nA / DC to 10 Hz
0.01 V/nA / DC to 1 kHz
Conversion Factor /
(Switchable)
Frequency Bandwidth
8 mm
Effective Area
+5 °C to +50 °C
Operating Ambient Temperature*
-20 °C to +50 °C
Storage Temperature*
Internal photodiodes
S1337-1010BQ S1337-1010BR S1337-1010BQ S1337-1010BR
* Using example: P.6
Spectral Response
1
TPMOB0213EA
* No condensation
H10799-001
Dimensional Outlines (Unit: mm)
0.1
H10799-102
14.0±0.2
25.0±0.5
EFFECTIVE AREA 8
H10799-002
0.01
MOUNTING HOLES FOR OPTIONS 4-M2
11
H10799-002 and H10799-102 are the values when
the current-to-voltage conversion factor is 0.1 V/nA
and the frequency bandwidth is DC to 100 Hz.
0.001
200
300
400
500
600
700
800
900
1000
WAVELENGTH (nm)
1100
18.0±0.5
RADIANT SENSITIVITY (V/nW)
H10799-101
GND
: AWG26 (BLACK)
Vcc LOW VOLTAGE INPUT: AWG26 (RED, +5 V)
SIGNAL OUTPUT
: RG-174/U
50.0±0.5
450±20
TPMOA0048EA
●ICCD Camera Units C10054 Series
C10054 series cameras boast high speed and high sensitivity thanks to
their built-in image intensifiers and high-speed electronic shutters
(min. 5 ns).
SPECIFICATIONS
Type No.
C10054-x1
C10054-x2
C10054-x3
C10054-x4
C10054-x5
C10054-x6
* Using example: P.21, P22, P24
Spectral Response Minimum Photocathode Limiting Resolution
(mm)
Illuminance (lx)
(TV Lines)
470
1 × 10-6
280 to 720
450
4 × 10-7
480
4 × 10-5
185 to 900
420
4 × 10-7
470
1 × 10-6
370 to 920
450
4 × 10-7
* Image intensifier: an imaging device with high light-intensification capabilities and
high-speed gate operation (shutter operation).
* There are separate catalogs for image intensifiers and ICCD cameras. Please
refer to those catalogs for more information.
27
Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office.
Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are
subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2014 Hamamatsu Photonics K.K.
HAMAMATSU PHOTONICS K.K.
www.hamamatsu.com
HAMAMATSU PHOTONICS K.K., Electron Tube Division
314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205
U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected]
Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected]
France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected]
United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, United Kingdom, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: [email protected]
North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected]
TPMO1058E01
Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-93581733, Fax: (39)02-93581741 E-mail: [email protected]
China: Hamamatsu Photonics (China) Co., Ltd.: B1201 Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected]
FEB. 2014 IP