OPTO OPTICAL BLOCKS MECHANICS Optical Components for Optical Measurement OVERVIEW FEATURES The optical blocks are compact units that contain components such as bandpass filters and dichroic mirrors. They are designed for lowlight-level measurements that use PMT modules and high-sensitivity cameras. Because their optical components are placed precisely through the use of high-precision machining, the blocks shield light superbly. Also, you can use the separate blocks in different combinations by removing and attaching them with thumbscrews. ●Easy to Attach / Detach ●No Alignment Required ●Superb Light-shielding Effect ●Makes Low-Light-Level Measurement Easy COMPARISON WITH BASIC OPTICAL DEVICES Mechanics Integration Holder Stage Optics Filter Mirror Lens Light shield Darkroom environment Detector PMT Photodiode Camera LINE UP Photo 2 Product Name / Type No. Over View Page Adapter Block A10030 Series The A10030 series is an adapter block for connecting a PMT module to a Vgroove type optical block. 4 C-Mount Adapter Block A10039 This block connects a device having C-mount to V-groove type optical blocks. The connection angle for the device and block is adjustable. 4 Fiber Adapter Block A10037 Series Optical fiber cables with FC or SMA connectors can be connected to the blocks in the A10037 series. The lens assembled in the block collimates the light spread from the optical fiber. 5 Beam Expander Block A10031 This optical block widens the diameter of a collimated beam of light to 2.5 times its original size or narrows the diameter to 1/2.5 times its original size. 5 Joint Block A10038 Series The A10038 series is a joint block for connecting one optical block to another. The joint is available in two types: MM (male-male) type and FF (female-female) type. 6 Cuvette Holder Block A11971 This holder block is a small dark box specifically designed for cuvettes with an optical path length of 10 mm, and allows easy measurement of samples in combination with various optical blocks. 6 Shutter Block A10036 The A10036 is a manual shutter block with a plate that is opened and closed by sliding it left and right. Light is sufficiently blocked so there will be no problem even at photon counting levels. 7 ND Filter Block A10032 Series A neutral density (ND) filter is included in this optical block to attenuate light levels. 7 Filter Block A10033 Series This optical block contains a filter that passes only particular wavelengths. Bandpass filters and long-pass filters are available. 8 Interchangeable Filter Block A10033-90 The A10033-90 is a holder block for assembling a commercially available 15 mm diameter filter. 9 Dichroic Block A10034 Series This block contains a dichroic mirror that reflects particular wavelengths but passes other wavelengths. A total of six products are available. 10 Beam Splitter Block A10035 This optical block uses a cubic type half-mirror. Incident light is split into reflected and transmitted beams at a ratio of 1 to 1. 11 Beam Splitter Holder Block A10035-90 The A10035-90 is a holder block designed to hold a 15 mm cubic type beam splitter. 11 Stage Block A10858 This is a dark box equipped with 2 micrometers for positionings. It allows light measurements even under room lighting by externally connecting various types of optical blocks. 12, 13 Beam Aligner Block A10760 This optical block is for using an infinity-corrected objective lens to guide laser light into an optical fiber with an attached FC connector or to collimate and transmit the beam from a fiber-optic cable. 14 Tube Lens Block A10859 Series This block contains a focusing lens with a focal distance of 100 mm and is used for an infinity-corrected objective lens. Aberration is reduced due to the use of an achromatic lens. 15 Pinhole Holder Block A11027 This is a feed screw type holder block that can hold a commercially available, mount type pinhole and adjust its position in the XY direction. Light passing through the pinhole is collimated by the lens installed in the block and is output. 16 C-Mount Interchangeable Filter Block A11213 Series The A11213 series is a C-mount connection block for installing a commercially available 25 mm diameter filter or lens. This block allows angle adjustment and so is useful as a spacer or adapter that connects between C-mounts. 17 Photo Product Name / Type No. Over View Page C-Mount Ring A9865 This ring is for connecting a PMT module to a optical block and an equipment with C-mount. 17 C-Mount Interchangeable Dichroic Block A11214 The A11214 is a C-mount connection block for installing a dichroic mirror for use at a 45° angle of incidence. 18 Polarizer Holder Block A11026 This is a holder block designed for a polarizer. Polarizing filters and waveplates are polarizing elements whose polarization characteristics change depending on direction and angle. 19 Parts Set A10455-02 This maintenance kit includes a set of screws and O-rings needed to assemble optical blocks. 19 CONNECTION EXAMPLES Page ●Connection Example Using Bifurcated Optical Fiber ......................................................................................................................... 20 ●Connection Example Using the Beam Expander Block ..................................................................................................................... 20 ●Connecting to a Microscope ............................................................................................................................................................... 20 ●Fluorescence Detection (Confocal) .................................................................................................................................................... 21 ●Connection Example Using an Objective Lens and the Tube Lens Block ..................................................................................................... 21 ●Imaging System Connection Example ............................................................................................................................................... 22 ●Emission Detection ............................................................................................................................................................................. 23 ●Example of Connection to a Mini-Spectrometer ................................................................................................................................. 23 ●Simultaneous Measurement with a PMT Module and an Imaging Camera ..................................................................................................... 24 ●Fluorescence Dye and Connecting Examples ..................................................................................................................................... 25 COMPATIBLE PMT MODULE Screw connection with PMT module Optical block with V-groove connector Optical block with C-mounts Optical block with V-groove connector Optical block with V-groove connector Optical block with C-mounts Adapter Block A10030-01 H10720/H10721 H10682 H10722/H10723 H11890 H11526 C-Mount Ring A9865 H10706/H12056 H10799 (built-in photodiode) H7826/H7827/H7828 Adapter Block A10030-02 C-mount connection with PMT module H7421/H7422 C-Mount Adapter Block A10039 C-mount adapter plate* H11461/H11462/H8259 * In preparation for use with optical blocks, a C-mount adapter plate must be attached to the PMT module. Please notify us of your need for this adapter when ordering. 3 BLOCK CHARACTERISTICS ●Adapter Block A10030-01, -02 The A10030-01 is an adapter block for connecting a PMT module to each block. The A10030-01 adapter block has M4 and M3 screw holes for securing to an optical bench or optical stage. A mini-post can also be attached to the M4 screw holes. Applicable mini-post: OptoSigma Model 155-0XXX SPECIFICATIONS Type No. Weight Effective Beam Size A10030-01 8 mm Approx. 18 g A10030-02 12 mm* * When connected to other optical blocks, their effective optical path diameters take precedence. Dimensional Outlines (Unit: mm) A10030-01 26.0±0.1 10.8±0.1 15.0±0.1 15.0±0.1 2-M3 DEPTH 3 (MOUNTING THREADED HOLE) M4 DEPTH 5 0.5 (MOUNTING THREADED HOLE) 9 H10720/H10721/H10722/H10723/H11706 A10030-01 H12056/H10682/H11890/H11526 H10799 (built-in photodiode) A10030-02 H7826/H7827/H7828 22.0±0.1 38.2±1.0 COMPATIBLE PMT MODULES GASKET 4-M2 (THUMB SCREW FOR PMT MODULE) 9 NOTE: Avoid frequently attaching and detaching from PMT module. 8 4 TPMOA0050EA A10030-02 27.0±0.1 18.0±0.1 10.5±0.1 15.5±0.1 GASKET 15.0±0.1 18.0±0.1 23.0±0.1 38.2±1.0 4-M2 (THUMB SCREW FOR PMT MODULE) 8 2-M3 DEPTH 4 (MOUNTING THREADED HOLE) M4 DEPTH 5 (MOUNTING THREADED HOLE) 0.5 4 TPMOA0085EA ●C-Mount Adapter Block A10039 One end of this adapter block has a C-mount (male threads) that connects to the H7421 series or H7422 series PMT module, while the other end couples to another optical block. This adapter block also connects to a commercially available device with a C-mount. This adapter block can also be connected to commercially sold Cmount products. SPECIFICATIONS Type No. A10039 Weight Approx. 27 g Effective Beam Size 8 mm * Supplied: C-mount double female thread ring. 4 2. Insert the C-mount ring into the C-mount of the PMT module and turn the adapter block body clockwise until it stops. 3. Turn the adapter block body counterclockwise so that it is positioned at the desired angle and then tighten the four screws. C-MOUNT (MALE) 32 22.0±0.1 1. Loosen the four screws on the adapter block and make sure the Cmount ring is separated about 1 mm from the adapter block body. 4 22.0±0.1 How to connect a C-mount adapter block 38.2±1.0 Dimensional Outlines (Unit: mm) 16.5±0.2 3.5±0.1 TACCA0287EA ●Fiber Adapter Block A10037 Series Optical fiber cables with FC or SMA connectors can be connected to the blocks in the A10037 series. The lens assembled in the block collimates the light spread from the optical fiber. The A10037/A10037-01 is suitable for use in the visible region (400 nm to 700 nm) and the A10037-10/A10037-11 for use in the UV region (200 nm to 400 nm). SPECIFICATIONS ▲A10037-01 Dimensional Outlines (Unit: mm) Transmittance (Typical) 22.0±0.1 TACCB0094EB (8) 60 ( 16) 80 TRANSMITTANCE (%) 7.0±0.1 22.0±0.1 100 Weight Recommended Wavelength Connecter Focal Length FC Visible SMA 10 mm Approx. 17 g FC UV SMA 4.6±0.1 ▲A10037 Type No. A10037 A10037-01 A10037-10 A10037-11 A10037/-10 : L=9.0 A10037-01/-11 : L=13.5 L 40 When fiber core diameter is 100 µm and NA is 0.2 Distance from Fiber Output End Beam Diameter 100 mm Max. 4.9 mm 150 mm Max. 5.4 mm 20 A10037 / A10037-01 A10037-10 / A10037-11 0 200 300 400 500 600 700 * In the case of the SMA connector type, the beam diameter depends on the position of the connected fiber cable end. The above values are measured when the fiber cable end is at the same position as the FC connector type. 800 WAVELENGTH (nm) TACCA0270EB ●Beam Expander Block A10031 This optical block widens the diameter of a collimated beam of light to 2.5 times its original size or narrows the diameter to 1/2.5 times its original size. This block has a C-mount thread for connecting to another device. SPECIFICATIONS Type No. Weight Effective Beam Size A10031 8 mm Approx. 43 g * Supplied: C-mount double male thread ring. ** Designed for wavelength from 400 nm to 700 nm. 36.0±0.2 TACCB0095EB ( 16) 100 9.0±0.1 Transmittance (Typical) 30.0±0.2 Input of parallel light collimated to a 20 mm or less Dimensional Outlines (Unit: mm) C-MOUNT (FEMALE) 20 400 500 600 700 800 44.4±0.3 8 8 TACCA0271EA ( 16) Incident light conditions 40 0 300 44.4±0.3 8 (PARALLEL) 60 20 (PARALLEL) TRANSMITTANCE (%) 80 WAVELENGTH (nm) TACCC0127EA 5 BLOCK CHARACTERISTICS ●Joint Block A10038 Series The A10038 series is a joint block for connecting one optical block to another. The joint is available in two types: MM (male-male) type and FF (female-female) type. Interposing the MM block (A10038-01) and/or FF block (A10038-02) between optical blocks allows various connections. SPECIFICATIONS Type No. A10038-01 A10038-02 Effective Beam Size 8 mm 8 mm Weight Approx. 10 g Approx. 25 g Dimensional Outlines (Unit: mm) 5.0±0.1 16 22.0±0.1 ▲A10038-01 9.0±0.1 A10038-01 22.0±0.1 (8) (8) 22.0±0.1 19.0±0.2 TACCA0284EA 38.2±1.0 22.0±0.1 A10038-02 ▲A10038-02 TACCA0285EA ●Cuvette Holder Block A11971 The A11971 cuvette holder block is a small dark box specifically designed for cuvettes with an optical path length of 10 mm. It allows easy measurement of samples in combination with other optical blocks. The base can be removed as needed. NOTE: This cuvette holder block is very compact. If it falls over, the sample may leak from the cuvette, so we recommend securing the body of the holder block and / or using a capped cuvette. SPECIFICATIONS Applicable Cuvette Size Effective Weight Beam Size Optical Path Length Dimensional Outline 12.5 mm × Approx. 206 g 8 mm 10 mm 56 mm (H) (including the base) Type No. CUVETTE HOLDER BLOCK Connection Example: Absorbance and Side Fluorescence Detection Using Optical Fiber A11971 Dimensional Outlines (Unit: mm) (25) PMT MODULE DASHED LINE CUVETTE 28.1 38.2 ± 1.0 ADAPTER BLOCK A10030-01 19.5 BLOCK CAP (10) 21 25.0 ± 0.1 PHOTOSENSOR MODULE H10799-xxx 18.0 ± 0.1 18.0 ± 0.1 CUVETTE HOLDER BLOCK + CUVETTE NOTE: Cuvette is sold separately. 8.1 2 6 8.1 20 16 14 ± 1 BASE MOUNTING SURFACE 50.0 ± 0.1 CUVETTE HOLDER BLOCK A11971 A' 32 15.0 ± 0.1 FIBER ADAPTER BLOCK A10037-xx 85 BASE (REMOVABLE) A 25.0 ± 0.1 2 × 4.5 (FOR HOLDING THE BASE IN PLACE) FILTER BLOCK A10033-xx 43.0 ± 0.2 22 21 56 22 8.1 BLOCK CAP 60 30 CROSS SECTION A–A' (WHEN BLOCK CAP IS REMOVED) M3 BOLTS WITH HEXAGONAL HOLES FOR ATTACHING THE BASE TPMOA0086EA ●Shutter Block A10036 The A10036 is a manual shutter block with a plate that is opened and closed by sliding it left and right. Light is sufficiently blocked so there will be no problem even at photon counting levels. SPECIFICATIONS Type No. Weight Effective Beam Size A10036 8 mm Approx. 48 g 22.0±0.1 -0.02 12.0±0.1 16.00 -0.05 24.0±0.1 9.0±0.1 NOTE: If you feel the shutter does not open and close smoothly, we recommend applying small amount of an optical grease to the plate. 49.0±0.2 11.0±0.1 21.0±0.1 22.0±0.1 38.2±1.0 Dimensional Outlines (Unit: mm) 2 16.0±0.1 30.0±0.1 (8) TACCA0288EA ●ND Filter Block A10032 Series A neutral density (ND) filter is included in this optical block to attenuate light levels. SPECIFICATIONS Type No. Built-in Filter Transmittance Effective Beam Size Weight A10032-10 10 % ND Filter 8 mm Approx. 26 g 1% A10032-11 * Light transmittance is the average transmittance at wavelengths from 400 nm to 700 nm. Dimensional Outlines (Unit: mm) 2 9.0±0.1 20.0±0.1 (8) Transmittance (Typical) 80 TACCB0087EA A10032-10 ( 16) 40 22.0±0.1 38.2±1.0 TRANSMITTANCE (%) 22.0±0.1 60 A10032-11 20 TACCA0272EA 0 300 400 500 600 700 WAVELENGTH (nm) 7 BLOCK CHARACTERISTICS ●Filter Block A10033 Series This optical block contains a filter that passes only particular wavelengths. Bandpass filters and long-pass filters are available. If you would like to use a filter with specifications other than those described below, purchase the A10033-90 interchangeable filter block (p. 9), which does not come with a preset filter. The A11213 C-mount interchangeable filter block series (p. 17) is also available. Filters with 25 mm diameters can be installed in these blocks. SPECIFICATIONS Type No. A10033-01 A10033-02 A10033-03 A10033-04 A10033-05 A10033-06 Built-in Filter Bandpass Filter Central Wavelength 530 nm ± 4 nm 610 nm ± 4 nm 630 nm ± 5 nm 460 nm ± 4 nm 575 nm ± 4 nm 700 nm ± 5 nm FWHM 40 nm ± 8 nm 40 nm ± 8 nm 60 nm ± 10 nm 60 nm ± 8 nm 30 nm ± 8 nm 60 nm ± 10 nm Effective Beam Size 8 mm Weight Approx. 25 g * Stopband: Below 0.01 % at 300 nm to 950 nm (except transmission bands) Type No. A10033-61 A10033-62 A10033-63 A10033-71 Built-in Filter Longpass Filter Cut-on Wavelength Effective Beam Size Weight 390 nm 500 nm 8 mm Approx. 25 g 600 nm 510 nm Transmittance [Bandpass filter] (Typical) A10033-01 A10033-02 TACCB0077EA 100 90 80 80 70 60 50 40 30 TRANSMITTANCE (%) 90 70 60 50 40 30 60 50 40 30 20 20 10 10 10 400 500 600 700 0 300 800 400 600 700 0 300 800 100 100 80 50 40 30 TRANSMITTANCE (%) 90 80 TRANSMITTANCE (%) 90 80 60 70 60 50 40 30 10 WAVELENGTH (nm) 800 400 800 30 20 700 700 40 10 600 500 600 WAVELENGTH (nm) 700 800 TACCB0082EA 50 10 500 800 60 20 400 700 70 20 0 300 600 A10033-06 TACCB0081EA 90 70 500 WAVELENGTH (nm) A10033-05 TACCB0080EA 0 300 400 WAVELENGTH (nm) A10033-04 100 500 TACCB0079EA 70 20 WAVELENGTH (nm) TRANSMITTANCE (%) 100 80 0 300 8 A10033-03 TACCB0078EA 90 TRANSMITTANCE (%) TRANSMITTANCE (%) 100 0 300 400 500 600 WAVELENGTH (nm) Transmittance [Longpass filter] (Typical) A10033-61, A10033-62, A10033-63 (Colored glass filter) 100 A10033-71 TACCB0083EA 100 90 90 80 80 A10033-61 A10033-62 A10033-63 TRANSMITTANCE (%) TRANSMITTANCE (%) TPMOB0214EA 70 60 50 40 30 70 60 50 40 30 20 20 10 10 0 300 400 500 600 700 0 300 800 400 500 600 700 800 WAVELENGTH (nm) WAVELENGTH (nm) * Transmittance characteristics are typical examples and may slightly vary depending on production lots. * Dimensional outlines are the same as the A10033-90. ●Interchangeable Filter Block A10033-90 How to install an optical filter The A10033-90 is a filter holder block that allows you to insert an optical filter according to your application. The filter is held by the spacer ring and the retaining ring, and can be easily exchanged by using the accessory tool. SPECIFICATIONS 1. Loosen the thumbscrews. 2. Insert, in order, the optical filter, spacer ring and retaining ring. Type No. Effective Beam Size A10033-90 8 mm Conpatible Filter Size Weight Diameter: 12.7 mm (1/2 inch) to 15 mm (Max.) Approx. (recommended: 15 mm) 24 g Thickness: 1.5 mm to 5 mm Dimensional Outlines (Unit: mm) 2 NOTE: • Filters for this block must be purchased separately. • Do not install optical filters in the wrong direction because they have the front side and back side for an light beam. Always insert the O-ring for blocking light after installing an optical filter. • When installing an optical filter, be careful to keep it clean and to avoid getting fingerprints on it. 9.0±0.1 20.0±0.1 (8) 4. Install the O-ring in place. 22.0±0.1 38.2±1.0 22.0±0.1 ( 16) 3. Tighten the retaining ring with the tightening tool. TACCA0272EA Connection Example: Fiber-Optic Single Wavelength Detection The lens inside the A10037 fiber adapter block collimates the beam of the light signal emitted from the optical fiber. The A10033 filter block only allows a specific wavelength to pass. When dimming the beam, use ND filter block A10032. FILTER BLOCK A10033-xx FILTER BLOCK A10033-xx PMT MODULE ND FILTER BLOCK A10032-xx PMT MODULE ADAPTER BLOCK A10030-01 FIBER ADAPTER BLOCK A10037-xx FIBER ADAPTER BLOCK A10037-xx ADAPTER BLOCK A10030-01 TPMOC0211EA 9 BLOCK CHARACTERISTICS ●Dichroic Block A10034 Series This optical block contains a dichroic mirror that reflects particular wavelengths but transmits other wavelengths. If you would like to use a mirror with specifications other than those described below, purchase the A11214 C-mount interchangeable dichroic block (p. 18), which does not come with a preset mirror. SPECIFICATIONS Built-in Dichroic Mirror DM505 DM565 DM600 DM660 DM430 DM455 Type No. A10034-01 A10034-02 A10034-03 A10034-04 A10034-05 A10034-06 NOTE: • Ultraviolet light shorter than 380 nm is absorbed by the coating materials deposited on the dichroic mirror, so the reflectance and transmittance significantly decrease. • For the reflected light, use the wavelength range where light is reflected more than 95 % (see the figures below). Effective Beam Size Weight 8 mm Approx. 57 g Transmittance (Typical) A10034-01 90 A10034-02 TACCB0084EA 100 Reflectance 95 % or more at 445 nm to 480 nm TRANSMITTANCE (%) TRANSMITTANCE (%) 60 50 40 30 60 50 40 30 70 60 50 40 30 20 20 10 10 10 500 600 700 0 300 800 400 WAVELENGTH (nm) 600 700 0 300 800 100 Reflectance 95 % or more at 460 nm to 625 nm 100 Reflectance 95 % or more at 380 nm to 405 nm 50 40 30 70 60 50 40 30 50 40 30 20 10 10 10 500 600 WAVELENGTH (nm) 700 800 400 500 600 700 Reflectance 95 % or more at 385 nm to 420 nm 60 20 400 800 70 20 0 300 700 TACCB0097EA 80 TRANSMITTANCE (%) TRANSMITTANCE (%) 60 600 90 80 70 500 A10034-06 TACCB0096EA 90 80 0 300 400 WAVELENGTH (nm) A10034-05 TACCB0093EA 90 500 WAVELENGTH (nm) A10034-04 100 Reflectance 95 % or more at 540 nm to 575 nm 80 70 20 400 TACCB0086EA 90 80 70 0 300 TRANSMITTANCE (%) 100 Reflectance 95 % or more at 510 nm to 545 nm 90 80 A10034-03 TACCB0085EA TRANSMITTANCE (%) 100 800 0 300 400 WAVELENGTH (nm) 500 600 700 800 WAVELENGTH (nm) * Dimensional outlines are the same as the A10035 (see the dimensional outlines on the next page). Connection Example: Fiber-Opic Dual Wavelength Detection PMT MODULE The lens inside the A10037 fiber adapter block collimates the beam of the light signal emitted from the optical fiber. The dichroic mirror inside the A10034 dichroic block reflects light below a certain wavelength and passes light above that wavelength. Finally, each of the two A10033 filter blocks isolates a specific wavelength so that the signal levels of the two separate wavelengths can be measured. FILTER BLOCK A10033-xx ADAPTER BLOCK A10030-01 FIBER ADAPTER BLOCK A10037-xx 10 ADAPTER BLOCK A10030-01 DICHROIC BLOCK A10034-xx ●Beam Splitter Block A10035 This optical block uses a cubic type half-mirror. Incident light is split into reflected and transmitted beams at a ratio of 1 to 1. SPECIFICATIONS Type No. A10035 Effective Beam Size 8 mm Weight Approx. 63 g * Designed for wavelength from 400 nm to 700 nm. NOTE: Light level is cut to approximately one third due to light absorption. 2 22.0±0.1 26.0±0.5 11.0±0.1 Dimensional Outlines (Unit: mm) Transmittance (Typical) ( 16) TACCB0089EC P ( 16) 60 38.2±1.0 30.0±0.2 80 AVERAGE 13.0±0.1 TRANSMITTANCE (%) 9.0±0.1 9.0±0.1 (8) 100 40 22.0±0.1 37.0±0.2 20 (8) TACCA0273EA S 0 400 450 500 550 600 650 700 WAVELENGTH (nm) ●Beam Splitter Holder Block A10035-90 The A10035-90 is a holder block designed to hold a 15 mm cubic type beam splitter. SPECIFICATIONS Type No. Effective Beam Size A10035-90 8 mm Applicable Beam Splitter Size Weight 15 mm × 15 mm × 15 mm cubic type Approx. 55 g * Supplied: Hex wrench Dimensional Outlines (Unit: mm) 2 26.0 NOTE: • The beam splitter is not included. • Be careful to install the beam splitter so that it faces in the correct direction. • When installing a beam splitter, be careful to keep it clean and to avoid getting fingerprints on it. DASHED LINE APPLICABLE BEAM SPLITTER SIZE 15 × 15 × 15 ( 16) (39) 17±0.1 (8) 4-M3 DEPTH 6 (FOR HOLDING THE BLOCK IN PLACE) 22.0 20.0±0.1 9.0±0.1 ( 16) 8 22.0±0.1 13.0 22.0 38.2±1.0 17±0.1 9.0±0.1 15.0±0.4 11.0±0.1 (26) 20.0±0.1 BOTTOM VIEW TPMOA0068EA 11 BLOCK CHARACTERISTICS ●Stage Block A10858 The stage block is for using an infinity-corrected objective lens in object image generation and fluorescence detection. It has two C-mount ports on the top and bottom of its rear panel. You can use other optical blocks to connect the stage block to a PMT module or ICCD camera (p. 27). Because you can use the stage block cover to keep the measured area in darkness, the stage block is useful even when detecting faint light. Furthermore, if you remove the cover and illuminate the sample appropriately using an illuminator, you can generate images by using the stage block with the A10859-01 tube lens block, CCD cameras, and other devices. SPECIFICATIONS Type No. A10858 Travels X-Axis Z-Axis Compatible Weight Objective Lens Infinity-corrected Objective lens Approx. ±6.5 mm ±3 mm 2; C-mount M20.32 × P0.706 4 kg Parfocal distance*: 45 mm Port * For information about the parfocal distance, see p. 14. Dimensional Outlines (Unit: mm) 50 140 110 (38) 160 81 Directions that you can move the workplate in using a micrometer. 245 135 2-C-MOUNT Direction of beam movement caused by beam steering mirror assembly adjustment Beam steering mirror assembly setup screws Accesories TPMOA0049EA Workplate and Beam Movement From outside of the stage block, you can use a micrometer to move the workplate horizontally along the X axis or vertically along the Z axis. You can also use the three setup screws in the beam steering mirror assembly to change the internal mirror angle and adjust the horizontal Y axis. Dimensional Outlines (Unit: mm) Stage Block Rack A11058 204 250 (127) (38) (ADJUSTABLE RANGE 25 to 38) 4-M6 204 Sizes of the included spacers 12 HEIGHT-ADJUSTMENT BOLT 8 Spacer 1 90 mm × 90 mm, Thickness: 10 mm 2 Spacer 2 60 mm × 60 mm, Thickness: 18 mm 1 Spacer 3 60 mm × 60 mm, Thickness: 25 mm 1 The spacer sizes have been designed according to their uses. (173) (ADJUSTABLE RANGE 160 to 173) 8 250 TPMOA0066EA Part Names and Features M6 Mounting Hole M6-thread mounting hole. You can use a mounting post or an angle mount for illumination. C-Mount Port There are two C-mount ports at the top and bottom of the stage block. You can connect the stage block to other optical blocks through blocks such as the A11213 C-mount interchangeable filter block. Workplate The stage block comes with three different workplates: A workplate with a 36 mm diameter hole. With this workplate, you can put the sample on a slide and observe its reflectedlight or transmitted-light image or measure its fluorescence. This workplate is specially designed for use with the A10657-01 microwell slide (sold separately). A workplate that you can place an object on to observe its reflected-light image in an upright configuration or to excite it from above and measure its fluorescence. * Please purchase illuminators from a company that manufactures them. Micrometer The micrometer enables you to perform precise horizontal (X-axis) and vertical (Z-axis) alignment and to accurately reproduce prior positionings. NOTE: Objective lens is not included. Spacer Objective Lens Mount A10921 A 20 mm spacer is installed between the workplate and the XZ axis stage. Removing this spacer will give you 20 mm of extra space, which is useful when observing relatively tall objects in an upright configuration. You can move the workplate along the X and Y axes by attaching an X-axis stage (SURUGA SEIKI B11-40C) in place of the spacer.* This is a mount for an objective lens. You can mount and remove the objective lens mount using the left and right screws. You can place the objective lens mount at the top of the stage mount for upright configurations, and at the bottom for inverted configurations. The A10858 comes with one objective lens mount. 45 mm Parfocal distance 45 mm * Please direct inquiries about the X-axis stage to SURUGA SEIKI. Micrometer height adjustment of ± 3 mm NOTE: An additional A10921 objective lens mount (sold separately) is attached in the above example. Stage clips are not included. Accesories Objective Lens Mount A10921 Microwell Slide A10657-01 * There is a separate catalog for microwell slides. Please refer to that catalog for more information. 13 BLOCK CHARACTERISTICS ●Beam Aligner Block A10760 This optical block is for using an infinity-corrected objective lens to guide laser light into an optical fiber with an attached FC connector or to collimate and transmit the beam from a fiber-optic cable. The block has two micrometers for positioning the optical fiber along the X and Y axes and an additional micrometer at the bottom for fine adjustments along the Z axis. This block has a cover around the objective lens and an additional cover for the entire block that further increases its light shielding capability. SPECIFICATIONS NOTE: Objective lens and fiber-optic cable are not included. Type No. Optical Fiber Travels XY-Axis Z-Axis Connector A10760 ±1 mm ±3 mm Compatible Weight Objective Lens Infinity-corrected Objective lens Approx. M20.32 × P0.706 420 g Parfocal distance: 45 mm FC * Supplied: Hex wrench USING EXAMPLE 1: FOCUSING BEAM ALIGNER BLOCK A10760 Dimensional Outlines (Unit: mm) OBJECTIVE LENS SCREW OPTICAL FIBER INFINITY-CORRECTED OBJECTIVE LENS C-MOUNT (FEMALE) TPMOC0209EB 69 USING EXAMPLE 2: COLLIMATOR 43 BEAM ALIGNER BLOCK A10760 OPTICAL FIBER INFINITY-CORRECTED OBJECTIVE LENS 70 52 TPMOC0210EA TPMOA0051EA Technical Guide Pupil Diameter and Parfocal Distance PUPIL DIAMETER OBJECTIVE MOUNTING POSITION PARFOCAL DISTANCE 1. Remove the two cover screws using a Phillips-head screwdriver, and then remove the cover. 2. Use the hexagonal wrench that comes with the block to remove the two plate screws, and then remove the plate. Objective lens Y-axis adjustment screw SAMPLE SURFACE TPMOC0223EA Pupil Diameter The diameter of the beam that is emitted from the pupil of an objective lens with a focal length f and a numerical aperture NA is given by: Pupil diameter = 2 × f × NA Parfocal Distance The parfocal distance is the distance from the objective mounting position of the objective lens to the sample at which the sample is in-focus. The A10760 is designed to be used with an objective lens whose parfocal distance is 45 mm. 14 X-axis adjustment screw 3. Attach an objective lens to the plate's objective lens screw hole (M20.32 P0.706). Z-axis adjustment screw 4. Reassemble the block by performing steps 1 and 2 in reverse, and then connect the block to other devices. You can align the optical fiber by using the X-, Y-, and Z-axis adjustment screws. Only turn the Z-axis adjustment screw after you have loosened the screws in step 1. ●Tube Lens Block A10859 Series ▲A10859 ▲A10859-01 Transmittance (Typical) 100 These are video lens blocks for use with the infinity-corrected objective lenses. It has a tube lens with a focal length of 100 mm to form an image. One side has a male C-mount thread, and the other side has a female C-mount thread. You can attach a device such as an ICCD or CCD camera to the side with the male thread. You can use a C-mount double male thread ring to connect the side with the female thread to the A10858 stage block. The tube lens block reduces aberration through the use of an achromatic lens. TPMOB0251EA TRANSMITTANCE (%) 80 60 40 SPECIFICATIONS 20 Type No. 0 300 400 500 600 700 800 900 Focal Length Effective Beam Size A10859 15 mm 100 mm A10859-01 24 mm * Supplied: C-mount double male thread ring. * Designed for wavelength from 400 nm to 700 nm. 1000 WAVELENGTH (nm) Dimensional Outlines (Unit: mm) A10859 A10859-01 C-MOUNT (FEMALE) MANUAL FOCUSING RING CAMERA ANGLE ADJUSTING SCREW* C-MOUNT (FEMALE) MANUAL FOCUSING RING CAMERA ANGLE ADJUSTING SCREW* C-MOUNT (MALE) 40 42 30 C-MOUNT (MALE) 32 Recommended Weight Sensor Size 1/3 inches or less Approx. 130 g 1/2 inches or less Approx. 205 g IMAGE PLANE 4 IMAGE PLANE 4 93 (92-94) 4 17.526 4 96 (95-97) TPMOA0052EA 17.526 * You can adjust the camera tilt by loosening this screw and turning the tube lens block. TPMOA0053EA Technical Guide Imaging Lens Distance Between the Imaging Lens and the Objective Lens The light from the sample is collimated by the infinitycorrected objective lens before it enters the imaging lens. The imaging lens focuses the image on the focal point. Even though the beam of light that passes from the infinity-corrected objective lens to the imaging lens is collimated, the distance between the lenses must still be kept below a certain limit. This limit (L) can be calculated from the specifications of the objective and imaging lenses and the effective area of the sensor. COLLIMATED LIGHT BEAM CAMERA L (mm) = INFINITY-CORRECTED OBJECTIVE LENS IMAGING LENS TUBE LENS BLOCK A10859 / A10859-01 f2(φ2 – φ1) φ φ1 = 2 × f1 × NA TPMOC0224EA Magnification You can calculate the magnification from the imaging lens focal length (100 mm) and the focal length of the microscope's objective lens. Imaging lens focal length 100 Magnification (M) = = Objective lens focal length Objective lens focal length Example: When the objective lens focal length is 45 mm, the magnification is approximately 2.2 times. d= φ M where φ1 is the objective lens's exit pupil diameter (mm), f1 is the focal length (mm), NA is the numeric aperture, φ2 is the imaging lens effective diameter, f2 is the focal length (mm), φ is the effective sensor area, and d is the observation area. To avoid vignetting when using the A10859 with objective lens (f1 = 18 mm, NA = 0.25) and a 1/3-inch video camera (φ = 6 mm), keep the distance from the objective lens to the A10859 below 100 mm. At this distance, placing a dichroic mirror between the objective lens and the A10859 will not have much of an effect on the overall optical characteristics. 15 BLOCK CHARACTERISTICS ●Pinhole Holder Block A11027 The A11027 is a holder block for mounting a pinhole. The screws can be used to adjust the pinhole's position in the XY directions. The built-in lens collimates the light that passes through the pinhole and converges the light on a photodetector. There is a base attached to the holder block through the M3 screw holes in the bottom of the block. If necessary, this base can be removed and re-attached to the side of the block. SPECIFICATIONS Type No. A11027 Diameter: 16 mm Thickness: 1.58 mm to 1.6 mm ±1.2 0.25 mm 15 mm Y-AXIS ADJUSTMENT SCREW ADJUSTMENT: ±1.2 C-MOUNT ANGLE ADJUSTMENT RING 2-M3 DEPTH 3 (FOR HOLDING THE BLOCK IN PLACE) 50.0 (REFERENCE PLANE OF THE PINHOLE MOUNT) 17.746 (27.526) (ASSUMED PINHOLE PLANE) 17.526 10.0 PINHOLE MOUNTING SCREW RING M4 DEPTH 5 (FOR HOLDING THE BLOCK IN PLACE) 40 50.0 4.6 ( 16) 29.0 45.0 (25) 43.0 12.0 5.0 22.95 30.0 (18) TRANSMITTANCE (%) 80 60 8.0 (0.22) 11.5±1.5 12.0 50.0 TPMOB0233EA 15.0±0.1 100 Approx. 180 g (including the base) Dimensional Outlines (Unit: mm) X-AXIS ADJUSTMENT SCREW ADJUSTMENT: ±1.2 Transmittance for the Collimation Lens (Typical) Weight * Supplied: Screw ring tool and hex wrench 11.5±1.5 NOTE: • The collimate status changes depending on the pinhole diameter and the wavelength. (The assumed wavelength is between 400 nm and 700 nm.) • The pinhole is not included. Use a pinhole that is installed in a mount. (Products by companies such as SIGMA KOKI CO., LTD.; SURUGA SEIKI CO., LTD.; and CVI Melles Griot can be used.) • Be careful to install the pinhole so that it faces in the correct direction. • When installing a pinhole, be careful to keep it clean and to avoid getting fingerprints on it. Applicable Pinhole XY Axis Range Adjustment Focal Mount Size of Motion Screw Pitch Length C-MOUNT (FEMALE) APPLICABLE PINHOLE MOUNT SIZE DIAMETER: 16. THICKNESS: 1.6 2-M3 BOLTS WITH HEXAGONAL HOLES FOR ATTACHING THE BASE BASE (REMOVABLE) 2- 4.5 (FOR HOLDING THE BASE IN PLACE) 60.0 BASE MOUNTING SURFACE TACCA0070EA 20 Extended Example of Microscope 0 300 400 500 600 WAVELENGTH (nm) 700 800 Connecting the A11027 pinhole holder block to the C-mount port of a microscope configures a confocal optical system. Light passing through the pinhole is collimated by the lens in the block and is efficiently guided to the PMT module. FILTER BLOCK A10033-xx PMT MODULE ADAPTER BLOCK A10030-01 PINHOLE HOLDER BLOCK A11027 MICROSCOPE C-MOUNT PORT MICROSCOPE 16 ●C-Mount Interchangeable Filter Block A11213 Series These C-mount threaded optical blocks are for installing commercial optical filters and lenses with 25 mm diameters. You can use it with the A11214 C-mount interchangeable dichroic block to measure fluorescence or perform other tasks. Because this block enables angle adjustment, it is also useful as a spacer between C-mounts and as a connection adapter. SPECIFICATIONS Type No. Effective Beam Size A11213 A11213-01 20 mm Weight Compatible Filter Size Diameter: 24 mm to 26 mm (25 mm to 25.4 mm recommended) Approx. 29 g Thickness: 5 mm (Max.) (2 mm to 5 mm recommended) Approx. 30 g ▲Left: A11213, Right: A11213-01 Connection Example: Single Wavelength Detection NOTE: • Filters and lenses for this block must be purchased separately. • When you install a filter that is designed to receive a beam of light from a specific side, make sure that you install the filter in the correct direction. • When installing an optical filter or other device, be careful to keep it clean and to avoid getting fingerprints on it. Only light at a specific wavelength passes through the optical filter assembled in the block and is detected by the PMT module. C-MOUNT RING A9865 Dimensional Outlines (Unit: mm) PMT MODULE A11213 (Male–Male Type) C-MOUNT (MALE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-xx 2 (DATUM PLANE OF FILTER) Connection Example: Fiber-Optic Single Wavelength Detection 35 Light emitted and spread from the optical fiber cable is collimated by the lens in the A10037 series fiber adapter block and is efficiently guided to the PMT module. 4 20 4 TPMOA0054EA C-MOUNT ADAPTER BLOCK A10039 A11213-01 (Male–Female Type) C-MOUNT (FEMALE) 2 (DATUM PLANE OF FILTER) C-MOUNT (MALE) PMT MODULE 35 C-MOUNT RING A9865 C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01 FIBER ADAPTER BLOCK A10037-xx 5 20 4 TPMOA0055EA ●C-Mount Ring A9865 This ring is for connecting a PMT module to a optical block and an equipment with C-mount. SPECIFICATIONS Type No. A9865 Effective Beam Size 8 mm Weight Approx. 7 g Dimensional Outlines (Unit: mm) GASKET C-MOUNT 9 30 4- 2.1 14 4- 3.2 COMPATIBLE PMT MODULES H10720/H10721/H10722/H10723/H11706/H12056/H10682 H11890/H11526 H10799 (built-in photodiode) 14 5.0 7.0 0.5 TPMOA0056EA 17 BLOCK CHARACTERISTICS ●C-Mount Interchangeable Dichroic Block A11214 This C-mount threaded optical block is for installing a dichroic mirror for use at a 45° angle of incidence. You can use typical dichroic mirrors that are commercially available for use with microscopes. SPECIFICATIONS Type No. Effective Beam Size A11214 20 mm Weight Compatible Dichroic Mirror Size Approx. 25 mm to 26 mm (W) × 35 mm to 38 mm (H) Thickness: 0.9 mm to 1.1 mm (1 mm recommended) 84 g * Supplied: Hex wrench 18 Dimensional Outlines (Unit: mm) 18 NOTE: • Dichroic mirrors for this block must be purchased separately. • Commercially sold dichroic mirrors usually have markings to distinguish their front and back sides. Be careful to install dichroic mirrors in the A11214 in the correct direction. • When installing a dichroic mirror, be careful to keep it clean and to avoid getting fingerprints on it. C-MOUNT (FEMALE) DEPTH 5 20 20 The dichroic mirror assembled in the block reflects light at wavelengths shorter than a specific wavelength and transmits longer wavelength light. Furthermore, only light at a specific wavelength passes through the optical filter and is then detected by the PMT module. Dual Wavelength Detection PMT MODULE PMT MODULE C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 C-MOUNT RING A9865 C-MOUNT RING A9865 C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 FIBER ADAPTER BLOCK A10037-xx C-MOUNT ADAPTER BLOCK A10039 Triple Wavelength Detection PMT MODULE C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 C-MOUNT RING A9865 C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 C-MOUNT ADAPTER BLOCK A10039 FIBER ADAPTER BLOCK A10037-xx 18 18 38 18 Connection Example: Fiber-Optic Dual / Triple Wavelength Detection 20 C-MOUNT (FEMALE) DEPTH 5 20 C-MOUNT (FEMALE) DEPTH 5 18 18 40 18 18 TPMOA0057EA ●Polarizer Holder Block A11026 Polarizing filters and waveplates are polarizing elements whose polarization characteristics change depending on direction and angle. The A11026 is a simple, course-movement holder block that can be used to adjust the direction and angle. SPECIFICATIONS Adjustable Angle Range Scale Applicable Polarizing Element Size Effective Type No. Beam Size Weight 5° 2 Diameter: 25 mm to 25.4 mm 90° 1 Approx. 55 g Thickness: 1 mm to 8 mm Intervals 1Can be rotated 90° or more by changing the angle adjustment screw position. 2Scale indicates adjustment intervals and does not indicate adjustable pitch. Can be fixed at any desired angle within the adjustable range of 90°. A11026 8 mm * Supplied: Hex wrench Dimensional Outlines (Unit: mm) ANGLE SCALE: 5° INTERVALS 34.0 22.0 ANGLE ADJUSTMENT RANGE 37.0±0.3 DASHED LINE APPLICABLE OPTICAL ELEMENT SIZE DIAMETER: 25.4. THICKNESS: 1 to 8 (8) 22.0 22.0 ( 16) °) (90 34.0 ANGLE ADJUSTMENT SCREW 9.0±0.1 38.2±1.0 (23±1) NOTE: • The polarizing filter or waveplate is not included. (Products by companies such as Edmund Optics Inc. and SIGMA KOKI CO., LTD. can be used.) • When installing a polarizing element, be careful to keep it clean and to avoid getting fingerprints on it. 32 2 (REFERENCE PLANE OF THE OPTICAL ELEMENT) 17 22.0 24 20.0±0.1 2-M3 DEPTH 3 (FOR HOLDING THE BLOCK IN PLACE) TACCA0069EA ●Parts Set A10455-02 This maintenance kit includes a set of screws and O-rings needed to assemble optical blocks. Parts Hex wrench Thumb screws Filter remove tool Screw-ring tool O-rings ( 15 mm) O-rings ( 26 mm) O-rings ( 28 mm) Screws M2 × 12 Screws M2 × 16 Hex socket setscrews M2 × 2 Hex socket setscrews M2 × 4 Hex socket setscrews M3 × 4 Hex socket bolts M2 × 8 Hex socket bolts M2.6 × 6 Hex socket setscrews M3 × 16 Washers * The A10030 is not currently sold. Qty. Optical blocks using parts at left 1 A10030*, A10035-90, A10760, A10858, A10859, A11027, A11213, A11214, A11971 (5 pcs/set) 4 1 1 2 1 1 10 10 10 10 10 10 10 5 3 A10030-01, A10030-02, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971 A10033-90 A11027 A10030-01, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971 A10039 A11026 A10030-01, A10030-02 A10039 A10035-90 A10030* A11213 A10030*, A10035-90, A11026 A11214 A11027 A11026 19 CONNECTION EXAMPLES ●Connection Example Using Bifurcated Optical Fiber Light from a light source is injected into one arm of the bifurcated optical fiber to irradiate a sample or measurement surface. Fluorescence emitted from the sample or light reflected from the measurement surface is collected by the same optical fiber and then detected by the PMT module through the other arm of the bifurcated optical fiber. FILTER BLOCK A10033-xx ADAPTER BLOCK A10030-01 PMT MODULE FIBER ADAPTER BLOCK A10037 BANDPASS LED FILTER TPMOC0213EA ●Connection Example Using the Beam Expander Block The A10031 beam expander block is designed to narrow a 20-mm beam of collimated light to a diameter of 8 mm, the size of the PMT module's photocathode; however, the expander block's use is not just limited to collimated light, it can also be used to converge divergent light. For example, if you use the beam expander block to receive the divergent light from an optical fiber, the signal level will be higher than if you had just used a PMT module. BEAM EXPANDER BLOCK A10031 ADAPTER BLOCK A10030-01 OPTICAL FIBER PMT MODULE DISTANCE L1 DISTANCE L2 <Measurement Conditions> Light source: Green LED Optical fiber: NA=0.5. Core diameter=0.98 mm Detection efficiency (Distance: 50 mm. Detection efficiency measured without beam expander block is normalized to 100.) DISTANCE (L1, L2) When Beam Expander is Connected (L1) When Beam Expander is not Connected (L2) 50 mm 80 % 100 % 90 mm 59 % 59 % 250 mm 28 % 13 % 350 mm 20 % 7% TPMOC0214EA ●Connecting to a Microscope The microscope port is designed so that the image is focused at 17.526 mm from the C-mount attachment location. When you connect a C-mount adapter to a commercially sold 10-mm C-mount ring (male to female), the approximate location of the image point is at the PMT module's photocathode (as shown in the figure on the left). If the light converges to a diameter of 1 mm or less at the image point, do not match the location of the image point to the location of the PMT module's photocathode; instead, expose the photocathode to a defocused light beam with a diameter of 1 mm or more. MICROSCOPE C-MOUNT RING (MALE-FEMALE) C-MOUNT RING A9865 17.526 PMT MODULE C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01 (SPACER) C-MOUNT RING A9865 MICROSCOPE 17.526 PMT MODULE L* 7 10 L* 7 25 * L is the distance from the attached end of the PMT module to the photocathode. 20 TPMOC0216EA ●Fluorescence Detection (Confocal) For detecting fluorescence, it is convenient to use a laser with an optical fiber output. If you intend to focus the laser up to the diffraction limit, use single-mode optical fiber. The infinity-corrected objective lens inside the beam aligner block collimates the light beam emitted from the optical fiber. In this situation, you can collect the light efficiently if you use an objective lens with an NA that is larger than that of the optical fiber. You can calculate the beam diameter from the optical fiber NA or the objective lens NA, whichever is smaller, and the focal length of the objective lens. The laser is reflected by the dichroic mirror, passes through the objective lens in the stage block, and excites the sample. The excited fluorescence passes through the objective lens, dichroic mirror, and tube lens block and is focused on the position of the pinhole that is installed in the pinhole block. This creates the confocal optical system. The light that passes through the pinhole is collimated by the installed lens and is injected into the PMT module. OPTICAL FIBER (FC CONNECTOR) BEAM ALIGNER BLOCK A10760 LASER OBJECTIVE LENS (COMMERCIALLY AVAILABLE) OBJECTIVE LENS (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) PINHOLE HOLDER BLOCK A11027 STAGE BLOCK A10858 PMT MODULE ADAPTER BLOCK A10030-01 FILTER BLOCK A10033-xx C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) PINHOLE (COMMERCIALLY AVAILABLE) TUBE LENS BLOCK A10859-01 C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 C-MOUNT DOUBLE MALE THREAD RING (SUPPLIED WITH A10859-01) DICHROIC MIRROR (COMMERCIALLY AVAILABLE) TPMOC0217EA Technical Guide The performance of the confocal optical system is determined by the size of the pinhole. The Airy disk (diameter) is used as the reference. The following equation is used to calculate the Airy disk. The system magnification is determined by the focal length of the observation-side objective lens and the focal length of the tube lens block. λ Airy disk (diameter) = 1.22 × M × NA Focal length of the tube lens block Focal length of the observation-side objective lens* λ : Fluorescence wavelength NA: NA of the observation-side objective lens M: System magnification = * If the objective lens focal distance on the observation side is unknown, please contact the lens manufacturer. If spatial resolution is important, use a pinhole whose size is close to the size of the Airy disk. If you are only measuring fluorescence intensity, make the pinhole larger than the Airy disk; this will lower the spatial resolution, but it will also increase the signal level. ●Connection Example Using an Objective Lens and the Tube Lens Block An infinity-corrected objective lens is connected to the A10859 tube lens block with a microscope objective lens mount, which has threads for a M20.32 × 0.706 mm objective lens and a C-mount male thread. Next, the tube lens block is connected to an imaging camera, such as an ICCD camera. You can adjust the focus using the manual focusing ring on the tube lens block. TUBE LENS BLOCK A10859 OBJECTIVE LENS ICCD CAMERA MICROSCOPE OBJECTIVE LENS MOUNT * (COMMERCIALLY AVAILABLE) * Example: Edmund Optics Microscope objective lens mount TPMOC0215EA 21 CONNECTION EXAMPLES ● Imaging System Connection Example Coaxial Incident Illumination Method Illuminate the sample with a fiber bundle connected to a xenon arc lamp. You can attach the fiber bundle easily by using a commercially sold C-mount light guide mount. To excite the sample, put an excitation filter that matches its fluorescent dye into the A11213-01 C-mount interchangeable filter block. If the light from the fiber bundle is not diffused enough, you can illuminate the sample evenly by inserting a holographic or other type of diffuser. The dichroic mirror reflects the excitation light. Then, the objective lens of the A10858 stage block focuses the light onto the sample, which is excited by the light. The fluorescence emitted from the sample passes through the dichroic mirror and the fluorescence filter and is focused onto the imaging camera by the lens inside the A10859-01 tube lens block. LIGHT GUIDE A10014-35-0110 XENON LAMP L9588-03 LIGHT GUIDE ADAPTER* (COMMERCIALLY AVAILABLE) EXCITATION FILTER (COMMERCIALLY AVAILABLE) C-MOUNT LIGHT GUIDE MOUNT* (COMMERCIALLY AVAILABLE) HOLOGRAPHIC DIFFUSER* (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01 FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE) EM-CCD or ICCD CAMERA OBJECTIVE LENS (COMMERCIALLY AVAILABLE) TUBE LENS BLOCK A10859-01 C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 DICHROIC MIRROR (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) STAGE BLOCK A10858 * Example: Edmund Optics •C-Thread Light Guide Mounting Adapter •Fiber Optic Light Guide Adapter SX-6 •Holographic Diffuser: 25 mm Type TPMOC0218EA Transmitted Illumination Method This is an imaging example that uses transmitted illumination. The image of the sample is focused onto an imaging camera, such as a CCD camera, by an infinity-corrected objective lens and the A10859-01 tube lens block. A convenient way to illuminate the sample is to use a commercially sold LED illuminator. C-MOUNT DOUBLE MALE THREAD RING (SUPPLIED WITH A10859-01) CCD CAMERA OBJECTIVE LENS (COMMERCIALLY AVAILABLE) TUBE LENS BLOCK A10859-01 SAMPLE LED LIGHT SOURCE STAGE BLOCK A10858 TPMOC0219JA 22 ●Emission Detection Collimated light such as chemiluminescence and bioluminescence captured by an infinity-corrected lens is reduced to 1/2.5 by using the A10031 beam expander and is guided to the PMT module with an effective diameter of 8 mm. A filter block is added when detesting light at a specific wavelength. SAMPLE C-MOUNT DOUBLE MALE THREAD RING (SUPPLIED WITH A10031) BEAM EXPANDER BLOCK A10031 OBJECTIVE LENS (COMMERCIALLY AVAILABLE) PMT MODULE ADAPTER BLOCK A10030-01 STAGE BLOCK A10858 FILTER BLOCK A10033-xx TPMOC0255EA ●Example of Connection to a Mini-Spectrometer The laser beam emitted from the optical fiber is collimated by the infinity-corrected objective lens inside the A10760 beam aligner block. The dichroic mirror inside the A11214 C-mount interchangeable dichroic mirror block reflects the collimated laser, which then passes through the objective lens in the A10858 stage block and illuminates the sample. The fluorescence from the excited sample is converged by the objective lens and then passes through the dichroic mirror inside the A11214. The fluorescence is then converged further by the A10031 beam expander block. The lens inside the fiber adapter block converges the light into the optical fiber and guides it to the mini-spectrometer, where it is measured. If you want to remove the excitation light, place the A10033 filter block between the beam expander block and the A10037-01 fiber adapter block. The fiber adapter block does not have focusing capabilities, so we recommend that you connect the fiber adapter block and the mini-spectrometer with an optical fiber whose core diameter is at least 600 µm. OPTICAL FIBER (FC CONNECTOR) BEAM ALIGNER BLOCK A10760 LASER OBJECTIVE LENS (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) STAGE BLOCK A10858 C-MOUNT DOUBLE MALE THREAD RING (SUPPLIED WITH A10031) JOINT BLOCK A10038-02 FIBER ADAPTER BLOCK A10037-01 OPTICAL FIBER (SMA CONNECTOR) (RECOMMENDED FIBER CORE DIAMETER : 600 µm or more) C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 BEAM EXPANDER BLOCK A10031 MINI SPECTOROMETER* DICHROIC MIRROR (COMMERCIALLY AVAILABLE) * There is a separate catalog for mini-spectrometers. Please refer to that catalog for more information. TPMOC0221EA 23 CONNECTION EXAMPLES ●Simultaneous Measurement with a PMT Module and an Imaging Camera The following is an example of simultaneous measurement in which the two A10921 objective lens mounts are attached to the A10858 stage block. The lower (inverted) part excites the sample using laser light and measures the fluorescence of the sample using the PMT module. The upper (upright) part illuminates the observation area using a xenon arc lamp and uses an imaging camera to observe the observation area and the fluorescence signal. A plano-convex lens and a fluorescence filter are installed in front of the PMT module to isolate the fluorescence. The plano-convex lens converges the beam of light. If this causes a problem in the fluorescence filter's wavelength shift, place the filter in front of the plano-convex lens. So that there is no interference with the PMT module's measurement, use a wavelength for the imaging camera's illumination that is different from both the excitation wavelength and the fluorescence wavelength. For example, if you use a 488 nm laser, use a DM505 dichroic mirror for the PMT module and a 530/40 bandpass filter for the emission filter. On the imaging camera side, use a 600 nm longpass filter for illumination and put a laser-blocking filter in front of the camera. LIGHT GUIDE A10014-35-0110 XENON LAMP L9588-03 LIGHT GUIDE ADAPTER* (COMMERCIALLY AVAILABLE) C-MOUNT LIGHT GUIDE MOUNT* (COMMERCIALLY AVAILABLE) 600 nm LONGPASS FILTER (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01 TUBE LENS BLOCK A10859-01 STAGE BLOCK A10858 OBJECTIVE LENS MOUNT A10921 EM-CCD or ICCD CAMERA OBJECTIVE LENS (COMMERCIALLY AVAILABLE) LASER-BLOCKING FILTER (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 C-MOUNT CUBE BEAMSPLITTER* (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214 DICHROIC MIRROR (COMMERCIALLY AVAILABLE) OBJECTIVE LENS (COMMERCIALLY AVAILABLE) C-MOUNT RING A9865 OBJECTIVE LENS MOUNT A10921 PMT MODULE FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01 C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR) PLANO-CONVEX LENS (COMMERCIALLY AVAILABLE) OBJECTIVE LENS (COMMERCIALLY AVAILABLE) C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 BEAM ALIGNER BLOCK A10760 OPTICAL FIBER (FC CONNECTOR) LASER * Example: Edmund Optics •C-Thread Light Guide Mounting Adapter •Fiber Optic Light Guide Adapter •C-Mounted Cube Beamsplitter NOTE: The A10858 stage block comes with one A10921 objective lens mount. To perform simultaneous measurement as shown above, you will need to purchase an additional A10921. TPMOC0222EA 24 ●Fluorescence Dye and Connecting Examples 1 ●Transmittance 633 nm 532 nm 100 A10034-04 DM 660 TACCB0090EA 90 A10033-05 575/30 nm OBJECTIVE LENS TRANSMITTANCE (%) 80 A10033-06 700/60 nm Cy3™ Alexa® 546 Cy5™ Alexa® 647 70 60 50 40 30 20 10 SAMPLE 0 400 500 600 700 800 700 800 700 800 TACCC0128EA WAVELENGTH (nm) 2 ●Transmittance 100 TACCB0091EA 90 80 EXCITATION LIGHT 405 nm A10033-04 460/60 nm TRANSMITTANCE (%) A10034-01 505 nm FLUORESCENCE A10033-01 530/40 nm 70 60 50 40 30 20 10 CASY FITC DAPI Hoechst 0 400 500 600 WAVELENGTH (nm) TACCC0129EA 3 ●Transmittance 100 TACCB0092EA 90 EXCITATION LIGHT 488 nm A10034-02 DM 565 A10033-01 530/40 nm FITC GFP A10034-03 DM 600 A10033-05 575/30 nm PE CASY A10034-04 DM 660 A10033-03 630/60 nm Texas Red® PI 80 A10033-06 700/60 nm Cy5™ 7AAD TRANSMITTANCE (%) FLUORESCENCE A10033-62 500 nmLP 70 60 50 40 30 20 10 0 400 500 600 TACCC0130EA WAVELENGTH (nm) 25 RELATED PRODUCTS ●Stabilized Light Sources For PMT L11494 Series The majority of these devices are LED light sources that have been developed for visual-sensitive compensation of PMTs and PMT modules. Because the amount of light generated by the LED is monitored and controlled by a photodiode, these devices always generate stable light of approximately 1 pW. SPECIFICATIONS L11494-430 L11494-470 L11494-525 L11494-660 Unit Parameter Peak Wavelength of 428 465 522 660 nm Generated Light FWHM 65 26 35 30 nm Wavelength Distribution of Generated Light (Typical) ●L11494-430 ●L11494-470 TACCB0114EA 1 1.2 0.6 0.4 0.2 TACCB0120EA 1 0.8 RELATIVE ENERGY RELATIVE ENERGY 0.8 Life Characteristic TACCB0115EA RELATIVE VALUE 1 0.6 0.4 0.8 0.6 0.4 0.2 0.2 0 350 400 450 500 550 600 650 700 0 350 750 400 450 WAVELENGTH (nm) 550 600 650 700 0 750 0 12 6 WAVELENGTH (nm) ●L11494-525 1 500 ●L11494-660 TACCB0116EA 1 18 24 30 TIME (h) Temperature Characteristic TACCB0119EA 2 TACCB0121EA 1.5 0.6 0.4 0.2 RELATIVE VALUE (%) 0.8 RELATIVE ENERGY RELATIVE ENERGY 0.8 0.6 0.4 1 0.5 0 -0.5 -1 0.2 -1.5 0 350 400 450 500 550 600 650 700 750 0 350 400 450 WAVELENGTH (nm) 500 550 600 650 700 750 -2 0 5 10 15 WAVELENGTH (nm) Connection Example 20 25 30 35 40 45 50 TEMPERATURE (°C) Dimensional Outlines (Unit: mm) 24 STABILIZED LIGHT SOURCE L11494-xxx 8.5 12 12 2-M2 14 JOINT BLOCK A10038-01 EMISSION AREA 7 ON OFF ON (Low intensity) (High intensity) 14 65 PMT MODULE ADAPTER BLOCK A10030-01 * There is a separate catalog for stabilized light sources. Please refer to that catalog for more information. 26 SWITCH BATTERY SR41 TACCA0311EA RELATED PRODUCTS ●Photosensor Modules H10799 Series The H10799 series modules are cable output modules with S13371010 series photodiodes and current-to-voltage conversion amplifiers. You can connect it easily to optical blocks and use it as a photodetector. SPECIFICATIONS H10799 Series Parameter Suffix -001 -002 -102 -101 Spectral Response 190 nm / 320 nm to 1100 nm (See spectral response) Input Voltage +5 V Max. Input Voltage +5.5 V +4.5 V Max. Output Signal Voltage 0.1 V/nA / DC to 100 Hz Current-to-voltage 1 V/nA / DC to 10 Hz 0.01 V/nA / DC to 1 kHz Conversion Factor / (Switchable) Frequency Bandwidth 8 mm Effective Area +5 °C to +50 °C Operating Ambient Temperature* -20 °C to +50 °C Storage Temperature* Internal photodiodes S1337-1010BQ S1337-1010BR S1337-1010BQ S1337-1010BR * Using example: P.6 Spectral Response 1 TPMOB0213EA * No condensation H10799-001 Dimensional Outlines (Unit: mm) 0.1 H10799-102 14.0±0.2 25.0±0.5 EFFECTIVE AREA 8 H10799-002 0.01 MOUNTING HOLES FOR OPTIONS 4-M2 11 H10799-002 and H10799-102 are the values when the current-to-voltage conversion factor is 0.1 V/nA and the frequency bandwidth is DC to 100 Hz. 0.001 200 300 400 500 600 700 800 900 1000 WAVELENGTH (nm) 1100 18.0±0.5 RADIANT SENSITIVITY (V/nW) H10799-101 GND : AWG26 (BLACK) Vcc LOW VOLTAGE INPUT: AWG26 (RED, +5 V) SIGNAL OUTPUT : RG-174/U 50.0±0.5 450±20 TPMOA0048EA ●ICCD Camera Units C10054 Series C10054 series cameras boast high speed and high sensitivity thanks to their built-in image intensifiers and high-speed electronic shutters (min. 5 ns). SPECIFICATIONS Type No. C10054-x1 C10054-x2 C10054-x3 C10054-x4 C10054-x5 C10054-x6 * Using example: P.21, P22, P24 Spectral Response Minimum Photocathode Limiting Resolution (mm) Illuminance (lx) (TV Lines) 470 1 × 10-6 280 to 720 450 4 × 10-7 480 4 × 10-5 185 to 900 420 4 × 10-7 470 1 × 10-6 370 to 920 450 4 × 10-7 * Image intensifier: an imaging device with high light-intensification capabilities and high-speed gate operation (shutter operation). * There are separate catalogs for image intensifiers and ICCD cameras. Please refer to those catalogs for more information. 27 Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2014 Hamamatsu Photonics K.K. HAMAMATSU PHOTONICS K.K. www.hamamatsu.com HAMAMATSU PHOTONICS K.K., Electron Tube Division 314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205 U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, United Kingdom, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] TPMO1058E01 Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-93581733, Fax: (39)02-93581741 E-mail: [email protected] China: Hamamatsu Photonics (China) Co., Ltd.: B1201 Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected] FEB. 2014 IP