Datasheet

FLOW CELLS
OVERVIEW
Hamamatsu provides a wide variety of precision glass products such
as flow cells. These are designed and manufactured based on our 60
years of experience in the production of vacuum tubes such as
photomultiplier tubes along with our well-established glass processing
technology. We welcome your requests for custom products.
-003
-002
FEATURES
●High dimensional accuracy: Minimum tolerance ±0.005 mm
●Available in various shapes
(polishing, grinding, gas processing, lens bonding)
●Materials: synthetic fused silica, synthetic black quartz, etc.
●Custom products with sample inlet/outlet mechanism available
-004
-001
-006
-005
APPLICATIONS
●Flow cytometry
●Blood analysis
●Imaging flow cytometry
●Particle counter
SPECIFICATIONS
Type No.
Material
Flow channel size
(mm)
J11020-000-001
Standard type. Suitable flow channel for detecting minute particles.
-002
Grinded type. Standard type with nipple-type outlet.
M0.25 ± 0.025
-003
Synthetic fused silica
-004
Trapezoid *2
-005
-006
Features
Synthetic fused silica *1
Synthetic black quartz
M1.0 ± 0.025
Standard type flow cell with a synthetic fused silica hemisphere lens attached
by UV glue.
Combined glass pipe with flow cell by gas-process.
Smooth laminar flow and hydrodynamic focusing.
Plate type.
Synthetic black quartz galss type. Less stray light and low fluorescence from material.
*1: Index of refraction of synthetic fused silica: nC (656 nm) 1.456, nd (587 nm) 1.458, nF (486 nm) 1.463
*2: Upper base: 0.2 mm
m ±0.03 mm, Lower base: 0.15 m
mm ±0.03
03 mm, Height: 0.2 mm ±0.03 mm
Customization options: Size, shape, etc.
Customization
With grinding 1
With grinding 2
High quality synthetic fused silica flow cells
With step
Large channel
Thin cover glass
Synthetic black quartz
With lens
Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office.
Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are
subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2016 Hamamatsu Photonics K.K.
FLOW CELLS
●Surface quality
Parameter
Optical active areas or flow channel
Other polished surfaces
Scratch
Width 0.004 mm Max.
Width 0.02 mm Max.
Dig
Size 0.025 mm Max.
Size 0.1 mm Max.
Bubble and inclusion
Size 0.025 mm Max.
Size 0.1 mm Max.
Chipping
Width 0.025 mm Max.
Width 0.1 mm Max.
Stain
Size 0.01 mm Max.
Size 0.1 mm Max.
●Dimensional outline (Unit: mm)
J11020-000-001
J11020-000-002
5 ± 0.1
15 ± 0.1
1
9 ± 0.1
A
1 ± 0.1
2 × EFFECTIVE AREA
1.5
6
0.25 ± 0.025
2 × 0.22
DETAIL A
TPMZA0005EC
J11020-000-003
TPMZA0006EC
J11020-000-004
5 ± 0.1
15 ± 0.1
R2.5 (LENS)
9.5
2.475
FLOW
CHANNEL
2 × 0.22
FLOW
CHANNEL
DETAIL A
8 ± 0.5
GLUED JOINT
35 ± 0.5
5 ± 0.5
A
4 ± 0.05
(6)
11 ± 1
2 × 0.22
60°
1
2 × EFFECTIVE AREA
(5)
2 × EFFECTIVE AREA
0.25 ± 0.025
1
0.22
6
2
4.475 ± 0.1
2 × EFFECTIVE AREA
2 × EFFECTIVE AREA
2 × EFFECTIVE AREA
0.25 ± 0.025
2.5
(6)
60°
4 ± 0.1
60°
4 ± 0.1
A
15 ± 0.1
9
2 × 0.22
5
2 × 0.22
5 ± 0.1
FLOW
CHANNEL
A
4 × EFFECTIVE AREA
0.25 ± 0.025
FLOW CHANNEL
DETAIL A
DETAIL A
TPMZA0007EC
TPMZA0008EC
J11020-000-006
SYNTHETIC
BLACK
QUARTZ
47
5.0 ± 0.1
4.0 ± 0.1
5.5
EFFECTIVE AREA
0.2 ± 0.03
4
16 MIN.
A
9
FLOW CHANNEL
2 × AREA
SYNTHETIC
BLACK QUARTZ
16 MIN.
(0.2)
TPMZA0014EB
0.2 ± 0.03
24
15.0 ± 0.1
5
26
1 ± 0.025
A
SYNTHETIC
BLACK
QUARTZ
1
55
2
2.2
1 ± 0.025
J11020-000-005
0.15 ± 0.05
EXPANSION A-A
TPMZA0009EC
●Other capabilities
Mirror coating
Transmittance of synthetic black quartz
Ex.: Aluminum mirror: reflectance characteristics
100
TPMZ0022EA
TPMZ0023EA
10
THICKNESS
0.3 mm
0.5 mm
0.7 mm
1.0 mm
80
70
FROM AIuminum SIDE
FROM AIuminum SIDE With SiO2 COAT
FROM GLASS SIDE
60
50
40
30
20
TRANSMITTANCE (%) Typ.
REFLECTANCE (%) Typ.
90
1.0
0.1
10
0
200
300
400
500
600
700
800
900
0
200
300
WAVELENGTH (nm)
400
500
600
700
800
WAVELENGTH (nm)
* The values on the above chart are sample values only
* The values on the above chart are sample values only
Optical thin film coating
Marking
Ex.: AR (Antireflection) coating: reflectance characteristics
5.0
<0.5 % @488 nm
<1.0 % @400 to 650 nm
4.5
REFLECTANCE (%) Typ.
● Serial number
● Part number
● Flow path diameter
TPMZ0024EA
4.0
405 nm
3.5
633 nm
488 nm 532 nm
3.0
2.5
2.0
1.5
1.0
0.5
0
350
400
450
500
550
600
650
700
WAVELENGTH (nm)
* The values on the above chart are sample values only
Clear polishing for grinded inner surface
Fusion welding
Ex.: Conical shape / 30 deg
Ex.: J11020-000-004
Ex.: Micro cylindrical / 0.5 mm diameter
FLOW CELLS
FLOW CELL ASSEMBLY J12800 SERIES
FEATURES
●Alignment free
●Can use commercial tube fitting
●Easy to install
●Repetition stability
●Stable laminar flow
TECHNOLOGIES
▲J12800-000-101
●Proposed assembly structure design
based on laminar flow model
●Flow simulation technology
●High pressure toughness
▲J12800-000-103
SPECIFICATIONS
Parameter
Flow channel
Outside dimension
Material
Outside dimension
Accuracy of position
Fitting of sheath port
Fitting of the other ports
Control pressure
Flow cell
Assembly
Unit
µm
mm
mm
µm
inch
inch
kPa
J12800-000-101
J12800-000-103
Please refer to
250 × 250
J11020-000-005
20.0 (H) × 5.0 (W) × 4.0 (D)
Synthetic fused silica
65.0 (H) × 46.0 (W) × 28.7 (D)
137.0 (H) × 59.0 (W) × 19.0 (D)
—
± 100
for 1/16ID tubing
for 1/8ID tubing
for 1/16ID tubing
-50 to +200
+10 to 70
●Dimensional outline (Unit: mm)
J12800-000-103
28.7
10
5
BARB FITTING
FOR 1/8”ID TUBING
IDEX: P-647/ETFE
(1/4-28 MALE SCREW)
4 × 3.5 THRU HOLE
3x BARB FITTING FOR 1/16”ID TUBING
IDEX: P-646/ETFE (1/4-28 MALE SCREW)
19
10
54
40
A
14
+0.00
FLOW CELL
7.8
5
55
2.2
E
13 14
1/16”OD, 0.15 mmID
NEEDLE / STAINLESS STEEL
26
10
90°
4 HOLES
FOR MOUNTING
(M3 or #5-40UNF)
10
(137)
(121.9)
4 × M3 THREADED HOLE
NOTES
1. Pressure resistance: 30 psi
2. Easy to assemble and disassemble for cleaning, etc.
24
14
65.5
69
SAMPLE INLET PORT
71.5
28
28.4
12
SHEATH INLET PORT
E
46
36
60.00 - 0.05
WASTE
INLET PORT
SECTION A-A
17
28
59
13.5
5.00 ± 0.05
FITTING FOR 1/16”OD TUBING
IDEX: P-420/PEEK FEMALE NUT,
P-250/PEEK FERRULE
HAMAMATSU PHOTONICS K.K.
4.00 ± 0.05
0.25 ± 0.025
0.250 ± 0.025
2.00 ± 0.025
REFERENCE DRAWING
OF J11020-000-005
0.1 A
F
27.0 ± 0.10
13
21
31
2.50 ± 0.025
8.00 ± 0.05
0.1 A
A
26
65
14
11.5
J12800-000-101
A
B
120°
FLOW CELL
SYNTHETIC FUSED SILICA: TOSOH ES
www.hamamatsu.com
HAMAMATSU PHOTONICS K.K., Electron Tube Division
314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205
U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected]
Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected]
France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected]
United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, United Kingdom, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: [email protected]
North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected]
TPMZ1011E03
Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-93581733, Fax: (39)02-93581741 E-mail: [email protected]
JAN. 2016 IP
China: Hamamatsu Photonics (China) Co., Ltd.: B1201 Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected]
Similar pages