P R O D U C T IVIS SpectrumBL N O T E Pre-clinical in vivo imaging Key Features • Ultra high-sensitivity to support in vivo bioluminescence, chemiluminescence and Cerenkov imaging • High-throughput (10 mice) imaging enablement • High resolution (to 20 microns) with 3.9 cm field of view • 3D diffuse tomographic reconstruction for bioluminescence • Co-register 3D optical data with microCT, PET/SPECT and MRI • NIST traceable absolute calibration • Optional upgrade path to an IVIS Spectrum for full fluorescence enablement High-Throughput In Vivo Bioluminescence Imaging System The IVIS® SpectrumBL is an advanced high-throughput 2D and 3D optical imaging system designed to improve quantitative outcomes of bioluminescence, chemiluminescence and Cerenkov in vivo imaging. The SpectrumBL supports 10 mice simultaneous imaging for true high-throughput imaging for longitudinal studies to support large cohorts of mice. It uses unique optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. High-Throughput Bioluminescence Imaging 120% more compounds can be evaluated annually in vivo. Getting more compounds screened for target or biomarker validation in vivo during the earlier stages of pre-clinical drug discovery significantly improves efficiency in later clinical stage development. The IVIS SpectrumBL provides the best in class bioluminescence sensitivity, a standard with all IVIS imaging systems, with the ability to image 10 mice (Figure 1) at once. The 10 mouse manifold that comes standard with SpectrumBL improves drug discovery workflow by reducing imaging times for longitudinal studies in half. The chart (Figure 1) shows that with SpectrumBL 120% more compounds evaluated in vivo annualy Compounds Evaluated 160 120 80 40 0 Figure 1. A representative image of ten mice being imaged in vivo. Chart on the right illustrates how significantly more drug candidates can be evaluated in vivo due to the high-throughput imaging capabilities of SpectrumBL. Bioluminescence Imaging - Best in Class In Vivo Sensitivity IVIS SpectrumBL has a cooled (-90 °C) camera with large CCD chip area and low F-stop for high sensitivity bioluminescent light detection. Image multiple bioluminescent reporters such as firefly luciferase, Renilla luciferase and bacterial luciferase A B in vivo at depth with rapidly and quantitatively. The ultra sensitive camera optics allows single cell detection, earlier monitoring of micrometastasis in vivo and track tumor development longitudinally in vivo. Other applications include infectious disease (Figure 3), stem cell tracking and toxicology. C Day 5 Day 12 Day 26 Day 6 Day 12 Day 15 Day 19 Day 26 Figure 2. Single cell detection of 4T1-luc2 cells injected subcutaneously in nude mice (A), monitoring NCI-H460-luc2 lung tumor growth by NCI-H460-luc2 (B) and metastasis post intracardiac injection of MDA-MA-231-luc2 cells longitudinally (C). Image Min = -33328 Max = 5.6754e+06 p/s ec/cm^2/sr 4 2 6 10 8 6 4 2 5 10 8 6 Color Bar Min = 60000 Max = 5e+06 Click # OC2 0050 21611 2737 Exp t ID: GFAP_Xen10 Wed, Fe b 16, 2005 11:28 :18 Pathog en: S. pne umo Xe n 10+lu ciferin Em filter=6 20 Ro ute of Infe ction: IC Bin :M (8), FOV4 .1, f2 , 30s Animal Number: m5 - closeup view of brain Came ra: IVIS 20 0 Be ta II, SI62 0EEV Time Point: 1 9h po st infe ction Image Min = -8289.7 Max = 1.1902e+07 p/s ec/cm^2/sr 4 2 10 6 8 6 4 2 10 5 8 6 Color Bar Min = 50000 Max = 5e+06 UTI 2 Pneumonia Click # OC2 0050 21611 2500 Exp t ID: GFAP_Xen10 Wed, Fe b 16, 2005 11:25 :36 Pathog en: S. pne umo Xe n 10+lu ciferin Em filter=5 00 Ro ute of Infe ction: IC Bin :M (8), FOV4 .1, f1 , 30s Animal Number: m5 - closeup view of brain Came ra: IVIS 20 0 Be ta II, SI62 0EEV Time Point: 1 9h po st infe ction Meningitis Figure 3. Tracking infection progression in models of UTI, pneumonia and meningitis. Cerenkov Imaging – Optimized Software Tools for Faster Workflow Advanced 3D Analysis with MicroCT Co-registration Tools Living Image® software brings IVIS technology to life by facilitating an intuitive workflow for image acquisition, analysis and data organization Living Image software in SpectrumBL has new features such as an imaging mode optimized for Cerenkov imaging. The software guides the user for optimal camera parameter settings for high signal-to-noise when detecting light emitted from radionuclide-injected animal subjects. Look deeper, see further, and take science to a new level of sophistication with the 3D technology from PerkinElmer. 3D diffuse luminescence tomography (DLIT) utilizes structured light data with bioluminescence images to reconstruct three dimensional representations of bioluminescent reporters. Take the next step and analyze 3D sources in an anatomical context with the Digital Mouse Atlas (Figure 5). The optional DyCE™ mode makes it very easy to set up biodistribution scans of radiopharmaceuticals. Spectrally unmix radionuclides from other light-emitting probes of very different spectra. The DyCE technique acquires a series of dynamic images following an injection of radionuclide. The location of major internal organs is derived by proprietary algorithms and displayed in minutes (Figure 4). The DyCE software module includes the Multi-View platform and software that extends the functionality of Living Image and is available for all IVIS systems. The tomography tool allows the quantification of the number of cells in a tumor and 3D co-registration of bioluminescent reporter. Seamlessly co-register 3D bioluminescence with Quantum FX microCT (Figure 6). C Figure 5. DLIT 3D reconstruction shows precise localization of GL261-luc2 brain tumor using digital mouse atlas. Figure 4. A mouse bearing a subcutaneous 4T1-luc2 tumor in its right flank was injected with 315 μCi of 18F-FDG intravenously. The animal was imaged dynamically starting 55 seconds post-injection to capture the distribution of 18F-FDG in the mouse body via Cerenkov light from positron emission. Day 17 Day 21 Day 24 Day 31 Figure 6. Optical-CT co-registration of osteolytic tumors with Quantum FX microCT. Mice were implanted with MDA-MB-231-luc-D3H2Ln cells by intracardiac injection. 3 Inside the IVIS SpectrumBL CCD Camera Imaging Chamber • Light-tight imaging chamber • Heavy-duty castors • Integrated gas anesthesia • LED lamps for photographic images Custom Lens, and Emission Filter Wheel Scanning laser assembly • Heated stage to maintain optimum body temperature • Electromagnetic door latch • Motor-controlled stage, filter wheel, lens position, and f-stop Gas Anesthesia Manifold Imaging Chamber Heated Shelf • Scanning laser for mouse alignment and surface topography CCD Camera • Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum • 13.5 micron pixels, 2048 x 2048 • 16-bit digitizer delivers broad dynamic range • CCD is thermoelectrically (Peltier) cooled to -90 °C, ensuring low dark current and low noise Custom-Designed Lens Soundproof Portable Cart, with a 25x34-inch Footprint • 6-inch diameter optics, f/1– f/8 • High-resolution – down to 20 microns The Spectrum Series platform is tailored to your workflow and is available in three models: IVIS SpectrumBL, IVIS Spectrum and IVIS SpectrumCT Features Animal Capacity IVIS SpectrumBL IVIS Spectrum IVIS SpectrumCT 10 mice 5 mice 5 mice 3 Fluorescence 3 Full Spectral Tunability 3 Epi-Illumination 3 Trans-Illumination 3 3D Fluorescence Tomography 3 3D Bioluminescence Tomography 3 3 Quantification 3 3 Absolute Calibration 3 3 * 3D Multimodal Co-Registration (PET, CT, MRI) 3 3 Bioluminescence 3 Integrated X-Ray and microCT 3 3 3 3 3 3 3 3 3 3 3 3 Compute Pure Spectrum - Spectral Unmixing 3 Optimized NIR Excitation Lightsource Extended NIR Range 150W Tungsten EKE N/A Detector type Camera Temp Imaging Pixels Accessory Line 1" Back-thinned, back-illuminated Grade 1 CCD -90°C 2048 x 2048 Isolation chamber, Anesthesia, calibration tools, phantom mice, Multimodality Software and Mouse Imaging shuttle, DyCE Imaging, Multi View Imaging * For bioluminescent reporters, chemiluminescent, and Cerenkov sources only 4 IVIS SpectrumBL- Complete Imaging Solutions XGI - 8 Anesthesia System Cat No. 118918 Animal Isolation Chamber Kit XIC - 3 Cat No. 123997 Mouse Imaging Shuttle Cat No. 127744 Animal Shield Kit XAS-3 Cat No. 119002 XWS - 260 Workbench Cat No. 119207 IVIS Syringe Injection System Cat No. 124633 Luciferin Luciferase Cell Lines Lentiviral Particles 5 Imaging System Components Specifications Heated Chamber Yes Gas Anesthesia Ports Yes Injector Ports Yes Imaging Chamber Interior Size 43 x 50 x 60 cm (W x D x H) Imaging System Space Requirement 203 x 163 x 214 cm (W x D x H) Power Requirements 20 Amps for 120 VAC or 10 Amps for 230 VAC Stage Temperature 2 0-40 °C Computer Quad Core 2.8 GHz, 12 GB, 1333 MHz DDR3, SDRAM, 2GB NVIDIA Quadro 4000 with 256 CUDA Cores, I TB hard drive, 20" flat screen monitor Optical Specifications Camera Sensor Back-thinned, back-illuminated Grade 1 CCD CCD Size 2.7 x 2.7 cm Imaging Pixels 2048 x 2048 Quantum Efficiency > 85% 500-700 nm; > 30% 400-900 nm Pixel Size 13.5 microns Min. Field of View (FOV) 3.9 x 3.9 cm Max. Field of View (FOV) 23 x 23 cm Min. Image Pixel Resolution 20 microns Lens f/1 – f/8 1.5x, 2.5x, 5x, 8.7x magnifications Read Noise < 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16 Dark Current (Typical) < 100 electrons/s/cm2 3D Tomography Software Included (Bioluminescent reporters only) CCD Operating Temperature -90 °C For more information, please visit our website at www.perkinelmer.com/invivo PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2013-2015, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 011478B_01PKI