LINEAR_DIMENSIONS LND-BA1K

DATA SHEET
D
LND-BA1K
ADDRESSABLE BIOSENSOR ARRAYS
GENERAL DESCRIPTION
FEATURES
The custom oligonucleotide microarray is
synthesized
using
a
semiconductor-based
electrochemical-synthesis
process.
Each
oligonucleotide probe is synthesized via a
platinum electrode that is independently controlled
by the synthesizer’s computer. Synthesis is based
on established phosphoramidite chemistry and
occurs at thousands of sites simultaneously
according to a computer algorithm that activates
only specified electrodes. Since physical
photolithographic masks or pre-built collections of
oligos are not involved in the process, all probes
can be easily changed without extra time or cost.

Microarray of Electrodes I2C or SPI
Addressable
Electrical Control for Each Array Element
and Segment
Mux up to Four High Impedance Input
Lines into Array or;
Read Array
2240 Array Sites
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APPLICATIONS
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mRNA
µRNA
SNP Genotyping
Re-Sequencing
Custom Array Concept
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
Chemistry of in-situ oligonucleotide synthesis on microelectrodes.
The Linear Dimensions chips are produced in a
form that the protected thymines are attached on
the surface of each electrode.
By computer-controlled electronics, selected
electrodes are turned onto form effective acidic
local environment around the corresponding
electrodes. The protection groups on thymines
are cleaved off in the acidic environment.
Microfluidic device supplies amidite solutions
which couples to the deprotected nucleotide to
form phosphodiester bond.
The chip surface is washed to remove any free
amidite on the solution.
The repetition of selected deprotection-couplewash step results in the synthesis of
oligonucleotides with custom-designed sequence.
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
Comparison between Linear Dimensions and Affymetrix chips
Two platform shares the in-situ nature of the oligonucleotide synthesis, but they differ in the principle of
deprotecting the protecting group of amidites. In contrast Linear Dimensions where the electric signal controls the
deprotection chemistry. Affymetrix chip uses photolithographic principle where the photomasks are used to
selectively illuminate the position where the next coupling should be performed.
The synthesis of Affymetrix chip needs set of photomasks designed and synthesized before the actual synthesis
of the chip. Thus, the change of custom design is more time- and resource-consuming compared to Linear
Dimensions chip where the only thing you need to do is to change the sequence file for the synthesis software.
Moreover, the state-of-the-art microfulics design and electronic controls enables the exact synthesis chemistry
that Linear Dimensions offers precise synthesis of oligonucleotide up to 50-mer, compared to 25-mer of
Affymetrix.
Affymetrix
Linear Dimensions
Photolithography controlled synthesis
Electronics controlled synthesis
Needs to produce masks for each base
sequence
Software controlled sequence
Up to 25-mer
Up to 50-mer
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
CustomArrayTM Chips are re-usable
The stripping of Linear Dimensions chip is based on chemical denaturation of DNA:DNA and DNA:RNA hybrids.
The stripping enables for users to perform hybridization up to 3 times.
Probes that are longer than 40-mer may not be stripped completely. Optimized for DNA and RNA targets labeled
with biotin, and with cy5.
Microarrays must be kept wet for re-usage. The figures 1-3 illustrate the efficiency of stripping.
Re-Usable Custom Array
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
Stripping kit
Die Image
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
Pin Description
Pin Number
Description
1
Feedback
2
V0
3
V1
4
V2
5
V3
6
IREF
7
GND
8
CLOCK
9
SS
10
MOSI
11
MISO
12
Vdd
Layout
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 
LND-BA1K
Orientation Options
 Linear Dimensions Semiconductor Inc.  22620 Ricardo Rd, Cupertino CA 95014 USA  tel 312.321.1810  fax 312.321.1830  www.lineardimensions.com 