QCM934 QCM bio-sensing system Simultaneous measurement of mass change and viscoelastic change index QCM bio-sensing system QCM934 can be simultaneously measured a small mass change as resonant frequency and the viscoelastic change as resonant resistance with high sensitivity and high resolution. Application examples •Measurement of biopolymer interactions, such as protein •Real-time monitoring of the formation of high molecular and decomposition •Evaluation of biocompatible materials like titanium •Evaluation of a lithium ion secondary battery with EQCM •Film thickness measurement in the plating •Gas analysis, such as humidity and smell substance •Quantitative evaluation of the detergency in surfactant •Structural change monitor of the polymeric material System Examples Flow Cell System Flow Cell System is controlled by the accuracy of the ± 0.02 ℃ the temperature of the flow type cell of contents 90 μL which was placed in the measuring chamber (QCM934-510). Continuous reaction of multiple samples can be monitored in real time by the sample flow at a constant rate by the pump. Batch reaction can be monitored by stop of pump. Example system configuration (QCM934-FLOWBASE, Laptop PC, Pump) Example Measurement of antigen and antibody using 1CH Flow Cell System ① ■QCM system: ② QCM934 1CH Flow Cell System (with pump) ■Quartz Crystal Resonator: QA-A9M-AU(M)(Au, 9MHz mirror) ■Temperature: 25 ℃ ■Speed of moving fluid: Approx. 30 μL/ Min ■Sample： (Antigen) 1.0mg/ mL rabit IgG ① (Antibody) 1.0mg/ mL mouse anti-rabit IgG ② QCM934 Features Resonance frequency with high sensitivity and high resolution Simultaneous measurement of resonance resistance for an indicator of viscoelastic change QCM934 is suitable for high sensitivity of mass change detection for use in bio-sensing field necessary in ng order by measuring 27MHz(3rd harmonic of 9MHz resonator) and 0.01Hz resolution. The viscosity and structural change of sample are indicated by measuring resonance resistance. Sauerbrey’s equation The system is compatible with various measurements ΔF: Frequency change F: Base frequency Δm: Mass change A: Area ρ: Density of resonator μ: Crystal shear stress n: Overtone order High-precision temperature control of the measurement chamber(QCM934-550) QCM934-550 is kept a constant temperature of QCM934-510 with an accuracy of ± 0.02 ℃ for stable measurement. QCM934 is 3 type systems, Flow Cell; advanced temperature control, Batch Cell; Dip/ Well type, and EQCM; EQCM with Potentiostat/Galvanostat Expansion supports up to 4 channels QCM934 can be simultaneously measured or independent with max. 4ch QCM934-210 by connecting the extension slot of QCM934-010. System Examples Batch Cell System Batch Cell System is connected with the cell of dip type (PTFE), well type (PTFE, PEEK and transparent vinyl chloride) and flow type (PTFE, PEEK and transparent VC) for various measurement purpose. It is necessary to add another incubator to keep the sample at a stable temperature in these systems. Example System configuration (QCM934-BATCHBASE, Laptop PC, QA-CL4/ QA-CL4PK/ QA-CL5) ○Dip Cell QA-CL3 ○Well Cell QA-CL4 QA-CL4PK ○Ｗell Cell QA-CL5 ○Flow Cell QA-CL6 QA-CL6PK QA-CL7 EQCM System High sensitive EQCM system is combined with many Potentiostat/Galvanostats with analog output Data logger Potentiostat/ Galvanostat with analog output Example System configuration (QCM934-EQCMBASE, Laptop PC, Stand, Lab jack) Resonator Measurement image There are many options available for a 9MHz AT-cut resonator design with the combination of 14 different electrode materials (Au, Pt, Ag, Al, C, Cu, Ni Mo, ITO, Si, SiO2, SUS316L, SUS304, Ti), surface finish (standard and mirror) and lead wire fixing (integrate and separate) An AC voltage is applied to the electrodes of resonator and it is resonance by the piezoelectric effect, the mass change of the surface (Δm) is proportional to the resonance frequency change (ΔF). The sensitivity is about 0.36ng/Hz(about 1.8ng/Hz/cm2) in case of 3rd harmonic of 9MHz. Au electrode Lead wire AT-cut crystal piece Specification □QCM934 base function: QCM934-010+QCM934-210 Item Description Number of Up to 4 QCM934-210 can be installed channnels Resonance frequency, resistance and Measuring item temperature in measurement chamber Resonance Resolution: 0.01 Hz frequency Frequency range: 25 MHz to 27 MHz Resonance Resolution: 0.01 Ω resistance Resistance range: Max. 2kΩ Temperature Range: 5 ℃ to 55 ℃ Resolution 0.01 ℃ Gate time 0.1 s/ 1 s/ 10 s Resonator 9MHz At-cut Oscillation within 1 Hz/ min stability 25 ℃ phosphate buffer in the stable Interface USB 2.0 (full speed) Input power AC 100 to 240 V, 50/60 Hz source Power Max. 90 VA consumption implementation of 4ch Connection cable 20 cm QCM934-260-200B length (Option) 30 cm QCM934-260-300B Dimensions 320 x 280 x 133 (WxDxH)[mm]* Weight Operation environmental temperature Compliant standards Approx. 5.5 kg 10 ℃ to 40 ℃ non-condensing CE marking EMC, low-voltage directive □Oscillation Circuit Unit: QCM934-310 Item Description Temperature Control: On/ Off control Dimensions 156 x 72 x 32 (WxDxH)[mm]* Weight Operation environmental temperature Approx. 0.6 kg 10 ℃ to 40 ℃ non-condensing □Measurement Chamber: QCM934-550 Item Description Temperature Control: On/ Off control Temperature 15 ℃ to 45 ℃ in case of environmental control range temperature -15 ℃ to +20 ℃ Temoperature ±0.02 ℃ stability Oscillation circuit unit built for batch cell Oscillation circuit system Dimensions 136 x 102 x 182 (WxDxH)[mm] * Weight Operation environmental temperature Approx. 1.5 kg □Flow Cell system: QCM934-510 Item Description Main body: Alminum alloy/ Polypropylene Material O-ring: Viton® Dimensions 41 x 34 x 19 (WxDxH)[mm]* Capacity 90μL Cell is flowed with solution by the pump and Usage connected with QCM934-550 □9MHz AT-Cut Quartz Crystal Resonator: QA-A9M-series Item Description Au, Pt, Ag, Al, C, Cu, ITO, Mo, Ni, Si, SiO2, Electrode SUS316L, SUS304,Ti material 300nm of electrode material is sputtered onto a Ti film groundwork Electrode area 5mmφ □Dip Cell: QA-CL3 Item Description Main body: PTFE, PVDF Material O-ring: Viton® Dimensions 25.5 x 20 x 12 (WxDxH)[mm]* Usage Solution or air □Well Cell: QA-CL4/ QA-CL4PK Item Description Main body: PTFE, PVDF(QA-CL4) Material PEEK(QA-CL4PK) Oring: Viton® Dimensions 25.5 x 20 x 12 (WxDxH)[mm]* Capacity Max. 750μl Cell is filled with solution or CL4 and CL4PK Usage are connected with RG100(Cell of EQCM) □Well Cell: QA-CL5 Item Description Main body: VC Material O-ring: Viton® Dimensions 25.5 x 20 x 12 (WxDxH)[mm]* Capacity Max. 250μl Usage Cell is filled with solution □Flow Cell: QA-CL6/ QA-CL6PK/ QA-CL7 Item Description Main body: PTFE, PVDF(CL6)/ Material PEEK(CL6PK)/ VC(CL7) O-ring: Viton® Dimensions 28.0 x 20 x 12 (WxDxH)[mm]* Capacity 90μl Usage Cell is flowed with solution by the pump * without a projection part 10 ℃ to 40 ℃ non-condensing Product names in this paper are trademarks or registered trademarks. The specification is subject to change without notice. Please be forewarned. SEIKO EG&G CO.,LTD Grande Bldgs., 2-26-9, Hatchobori, Chuo-ku, Tokyo 104-0032, Japan TEL:(+81)3-5543-3106 FAX:(+81)3-5542-3109 http://www.sii.co.jp/segg Jun 2015 Rev. 1.1