3 CCD Cooled Digital Color Camera R ORCA-3CCD 3CCD Cooled Digital Color Camera ▲ Rear cable mount model (Type number : C7780-20) The ORCA-3CCD cooled digital color camera incorporates three cooled CCD chips, providing the rapid readout, high resolution and superior S/N ratio of the Hamamatsu ORCA digital camera series. The three color CCDs employ an RGB prism to achieve extremely high quality color representation without color blur, a performance difficult to achieve with a single-CCD camera. The CCDs used are the same as those used in the ORCA series, providing proven high quantum efficiency and high resolution, cooled to 0 °C for high sensitivity detection. It is suitable for a wide range of applications, from brightfield (i.e., stained pathological specimens) to fluorescent specimens using GFP and fluorescent antibodies. FEATURES Total 4.13 million pixels Total 36 bit color resolution Cooling temperature of 0 ˚C Individual R, G, and B exposure settings 8 × 8 binning capability 9.1 frames/s full speed display Low readout noise (13 electrons rms typ.) APPLICATIONS Color digital time-lapse recording Fluorescence Resonance Energy Transfer (FRET) studies Fluorescence In Situ Hybridization (FISH) studies Simultaneous imaging of multiply-labeled cell and tissue ▲ Sample: FluoCells Prepared Slide #1 High performance R, G, and B separation The ORCA-3CCD incorporates three color CCDs with a RGB prism to achieve color separation superior to that available with a conventional single CCD with mosaic filter for microscope use. Trichrome stained DAPI (blue), BODIPY FL (green), and MitoTracker (red) specimens were observed simultaneously using a D-F-T triple band mirror cassette. Separation of the color image into B, G, and R revealed admixture of BODIPY FL (green) fluorescence in the B (blue) and R (red) channels when using the single CCD with mosaic filter; however when the ORCA3CCD was used, skillful matching of the fluorescent dyes with the wavelength separation prism achieved excellent color separation. Blue Green Green PC Camera head Camera controller Image input board Lens ORCA-3CCD Microscope Image acquisition software Commercially available image processing software 2/3-inch ENG mount TV camera adapter Standard Optional SPECIFICATIONS Red ▲Color separation of single CCD Blue SYSTEM CONFIGURATION Red ▲Color separation of ORCA-3CCD Total 4.13 million pixels The use of three color CCDs in the ORCA-3CCD eliminates the deterioration in B (blue) and R (red) resolution which tends to occur with single color CCDs. The high spatial resolution of approximately 1.3 mega pixels for each of the R, G, and B channels allows acquisition of highly detailed fluorescent images. Cooling temperature of 0 ˚C The ORCA-3CCD employs Hamamatsu's own cooling technology* to lower the temperature of the CCDs to 0 ˚C. This significantly reduces the noise characteristics often associated with long exposures, thus allowing observation of faint levels of fluorescence. Model name Type number Signal output connector position Sensor structure Imaging device Effective number of pixels Cell size Effective area Pixel clock rate Frame rate 2 × 2 binning 4 × 4 binning 8 × 8 binning Readout noise (rms) Full well capacity Dynamic range* Cooling method Cooling temperature Dark current A/D converter Output signal (digital output) Exposure time External control Sub array External trigger Lens mount Ambient storage temperature Ambient operating temperature Ambient operating/storage humidity Line voltage Power consumption ORCA-3CCD C7780-10 C7780-20 Front Rear 3 chip CCD with RGB prism Progressive scan interline CCD 1344 (H) × 1024 (V) 6.45 mm × 6.45 mm (square format) 8.67 mm × 6.60 mm (2/3-inch format) 16 MHz/pixel 9.1 frames/s 18.1 frames/s 31.8 frames/s 51.5 frames/s 13 electrons 18,000 electrons 1384 : 1 Peltier cooling with air radiation 0 ˚C at +20 ˚C ambient temperature 0.5 electrons/pixel/s 12 bit 12 bit, 10 bit and 8 bit × 3 channels parallel output 128 μs to 10 s RS-232C (full remote for all camera functions) yes yes 2/3-inch bayonet mount (flange back 48 mm) -10 ˚C to + 50 ˚C 0 ˚C to + 40 ˚C 70 % max. (no condensation) 100 V / 117 V / 220 V / 240 V, 50 Hz/60 Hz Approx. 140 VA * Calculated from the ratio of the full well capacity and the readout noise. ▲ Non-cooled digital color camera Yellow circle: Location of dark noise ▲ ORCA-3CCD ★ ORCA is registered trademark of Hamamatsu Photonics K.K. (France, Germany, Japan, U.K., U.S.A.) ★ Windows is registered trademark of Microsoft Corporation in the U.S.A. ★ Product and software package names noted in this documentation are trademarks or registered trademarks of their respective manufacturers. ● ● Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult your local sales representative. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearance are subject to change without notice. © 2013 Hamamatsu Photonics K.K. HAMAMATSU PHOTONICS K.K. www.hamamatsu.com HAMAMATSU PHOTONICS K.K., Systems Division 812 Joko-cho, Higashi-ku, Hamamatsu City, 431-3196, Japan, Telephone: (81)53-431-0124, Fax: (81)53-435-1574, E-mail: [email protected] U.S.A.: Hamamatsu Corporation: 360 Foothill Road, P. O. Box 6910, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road Welwyn Garden City Hertfordshire AL7 1BW, United Kingdom, Telephone: 44-(0)1707-294888, Fax: 44(0)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Thorshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] Italy: Hamamatsu Photonics Italia: S.R.L.: Strada della Moia, 1/E, 20020 Arese, (Milano), Italy, Telephone: (39)02-935 81 733, Fax: (39)02-935 81 741 E-mail: [email protected] China: Hamamatsu Photonics (China) Co., Ltd.: 1201 Tower B, Jiaming Center, 27 Dongsanhuan Road North, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected] Cat. No. SCAS0087E01 FEB/2013 HPK Created in Japan