EasyTag Express Protein Labeling Mix, [ S]-

TECHNICAL
DATA
SHEET
35
S
Caution: For Laboratory Use. A product for research purposes only.
EasyTag™ Express Protein Labeling Mix, [35S]Product Number: NEG772
LOT SPECIFIC INFORMATION
Lot Number:
________ 03281 ___________
Specific Activity:
L-[35S] Methionine: 43.5 TBq/mmol (1175.0 Ci/mmol )
L-[35S] Cysteine: 39.8 TBq/mmol (1075.0 Ci/mmol )
Concentration:
Total Hydrolysate: 407.0 MBq/ml (11.0 mCi/ml )
L-[35S] Methionine: 296.0 MBq/ml (8.0 mCi/ml )
L-[35S] Cysteine: 88.8 MBq/ml (2.4 mCi/ml )
Calibration Date:
_______ 22-Apr-2011 ______
CAUTION: The package size of NEG-772 refers to the total radioactivity due to L-[35S] methionine, L-[35S]cysteine and
other 35S components. Pre-calibration significantly increases the actual mCi/ml and mCi/vial. Refer to the Product
Specifications section for distribution of labeled components.
PACKAGING: Stabilized aqueous solution.
PRODUCT SPECIFICATIONS: NEG-772, Easy TagTM EXPRESS is prepared directly from the hydrolysate of E.
coli grown in the presence of carrier-free [35S] sulfate with a proprietary stabilizer added. As determined by amino acid
analysis, the distribution of 35S-labeled components is routinely 73% L-[35S] methionine and 22% L-[35S] cysteine.
STABILITY AND STORAGE:
•
•
The rate of decomposition of NEG-772 Easy TagTM EXPRESS is approximately 1% per week when stored at 4oC.
The compound may be stored in a refrigerator during frequent use, and it may be exposed to room temperature for
several days without significant loss of purity or function. If used infrequently, freezing is recommended to
minimize the risk of microbial contamination.
HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause
cancer.
PRODUCT APPLICATION: NEG-772 is intended solely for use in eukaryotic or bacterial cell labeling studies. It
is not recommended or warranted for use in cell-free protein translation. The use of NEG-772 in the latter application
provides irreproducible experimental results, with significant variation in both 35S-amino acid incorporation and background
levels.
Page 1 of 2
SAFE HANDLING: Because this product has been stored at -80°C, it is possible that pressure may develop in the vial
during the thawing process. In addition, volatile 35S-labeled decomposition products are generated at a rate of ~0.01% per
week. We recommend that prior to opening, vials are first vented in a fume hood using the following procedure:
•
•
•
•
Slide aside the dust cover on the cap to expose the septum.
Pierce the septum with a cotton-plugged syringe needle or charcoal trap (NENTM NEX-033T), taking care that the
tip does not come in contact with the product.
If the product is frozen, quickly thaw at room temperature or in a 37°C water bath. Any pressure developed will
vent through the syringe needle.
Remove the needle and dispose of as contaminated equipment.
When used for in vivo labeling experiments, we recommend that specific steps be taken to minimize incubator and water bath
contamination. We suggest using a shallow tray of activated charcoal, charcoal sticks or charcoal filter units to trap 35S volatiles
and reduce contamination.
SPECIAL INFORMATION:
Decay of 35S (physical half-life, 87.4 days):
DECAY FACTORS
Days BEFORE
Assay Date
30
20
10
0
Days AFTER
Assay Date
0
10
20
30
1
2
3
4
5
1.269
1.279
1.181
1.091
1.299
1.200
1.109
1.000
1.008
1.016
1.024
1.309
1.210
1.117
1.032
1.320
1.172
1.083
1.289
1.191
1.100
2
3
4
0
0
1
1.219
1.135
1.049
5
6
1.330
1.229
1.135
1.049
7
1.352
1.249
1.153
1.057
1.066
6
9
0.931
0.860
0.795
0.734
0.992
0.984
0.976
0.969
0.961 0.954
0.946
0.939
0.924
0.853
0.788
0.916
0.847
0.782
0.909
0.840
0.776
0.902
0.833
0.770
0.895
0.827
0.764
0.888 0.881
0.820 0.814
0.758 0.752
0.874
0.807
0.746
0.867
0.801
0.740
from the specific activity at the calibration date (SAo ) and the decay factor (f) given above.
f
1/SAo
(1-f)/1494
RELATED PRODUCTS:
Easy TagTM Methionine,L-[35S]Methionine, L-[35S]- Premium Stabilized Grade
Methionine, L-[35S]- High concentration.
Methionine, L-[35S]- Translation Grade
Cysteine, L-[35S]Charcoal Trap for NENSureTM vial.
0695:121007
PerkinElmer, Inc.
549 Albany Street
Boston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
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All other trademarks are the property of their respective owners.
1.259
1.163
1.074
8
1.000
SA t =
9
1.362
7
The specific activity at time t (SA t) may be calculated, using the following equation,
NEG-709A
NEG-009A
NEG-009C
NEG-009T
NEG-022T
NEX-033T
8
1.341
1.239
1.144