Expre35s35s, [ S]-Protein Labeling Mix

TECHNICAL
DATA
SHEET
35
S
Caution: For Laboratory Use. A product for research purposes only.
Expre35s35s, [35S]-Protein Labeling Mix
Product Number: NEG072
LOT SPECIFIC INFORMATION
Lot Number:
________ 01241 ___________
Specific Activity:
L-[35S] Methionine: 43.5 TBq/mmol (1175.0 Ci/mmol )
L-[35S] Cysteine: 39.8 TBq/mmol (1075.0 Ci/mmol )
Concentration:
Total Hydrolysate: 407.0 MBq/ml (11.0 mCi/ml )
L-[35S] Methionine: 296.0 MBq/ml (8.0 mCi/ml )
L-[35S] Cysteine: 88.8 MBq/ml (2.4 mCi/ml )
Calibration Date:
______ 18-Feb-2011 _______
CAUTION: The package size of NEG-072 refers to the total radioactivity due to L-[35S]methionine, L-[35S]cysteine and
other 35S components. Pre-calibration significantly increases the actual mCi/ml and mCi/vial. Refer to the Product
Specifications section for distribution of labeled components.
PACKAGING: 50mM Tricine-HCl (pH 7.4) containing 10µmoles 2-mercaptoethanol per ml, in a NENSURE vial with
polypropylene-v-insert, shipped in dry ice.
PRODUCT SPECIFICATIONS: NEG-072, EXPRE35S35S is prepared directly from the hydrolysate of E. coli
grown in the presence of carrier-free [35S] sulfate. As determined by amino acid analysis, the distribution of 35S-labeled
components is routinely 73% L-[35S] methionine and 22% L-[35S] cysteine.
STABILITY AND STORAGE:
•
•
•
The rate of decomposition of NEG-072, EXPRE35S35S is approximately 1% per week when stored at -80oC.
Storage at higher temperatures accelerates product decomposition.
Both L-[35S] methionine and L-[35S] cysteine are highly susceptible to oxidation. Precautions should be taken to
minimize exposure to air.
If the entire quantity will not be used at once it is recommended that the product be thawed quickly, aliquoted under
N2 into samples of an appropriate size, refrozen immediately and stored at -80°C or below
HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause
cancer.
Page 1 of 2
PRODUCT APPLICATION: NEG-072 is intended solely for use in eukaryotic or bacterial in vivo labeling studies.
It is not recommended for use in cell-free mRNA-directed translations. The use of NEG-072 in the latter application provides
irreproducible experimental results, with significant variation in both 35S-amino acid incorporation and background levels.
SAFE HANDLING: Because this product has been stored at -80°C, it is possible that pressure may develop in the vial
during the thawing process. In addition, volatile 35S-labeled decomposition products are generated at a rate of ~0.01% per
week. We recommend that prior to opening, vials are first vented in a fume hood using the following procedure:
•
•
•
•
Slide aside the dust cover on the cap to expose the septum.
Pierce the septum with a cotton-plugged syringe needle or charcoal trap (NENTM NEX-033T), taking care that the
tip does not come in contact with the product.
If the product is frozen, quickly thaw at room temperature or in a 37°C water bath. Any pressure developed will
vent through the syringe needle.
Remove the needle and dispose of as contaminated equipment.
When used for in vivo labeling experiments, we recommend that specific steps be taken to minimize incubator and water bath
contamination. We suggest using a shallow tray of activated charcoal, charcoal sticks or charcoal filter units to trap 35S volatiles
and reduce contamination.
SPECIAL INFORMATION:
Decay of 35S (physical half-life, 87.4 days):
DECAY FACTORS
Days BEFORE
Assay Date
30
20
10
0
Days AFTER
Assay Date
0
10
20
30
1
2
3
4
5
1.269
1.279
1.181
1.091
1.008
1.299
1.200
1.109
1.309
1.210
1.117
1.320
1.172
1.083
1.000
1.289
1.191
1.100
1.016
1.024
1.032
1.049
2
3
4
7
8
9
0.992
0.984
0.976
0.969
0.961 0.954
0.946
0.939
0.916
0.847
0.782
0.909
0.840
0.776
0.902
0.833
0.770
0.895
0.827
0.764
0.888 0.881
0.820 0.814
0.758 0.752
0.874
0.807
0.746
0.867
0.801
0.740
0.931
0.860
0.795
0.734
0
0
1.000
0.924
0.853
0.788
1
1.219
1.135
5
6
1.330
1.229
1.135
1.049
7
1.341
1.239
1.144
1.057
1.066
1.074
6
from the specific activity at the calibration date (SAo ) and the decay factor (f) given above.
f
1/SAo
(1-f)/1494
RELATED PRODUCTS:
NEG-009A
NEG-009C
NEG-009T
NEG-022T
NEX-033T
Methionine, L-[35S]- Premium Stabilized Grade
Methionine, L-[35S]- High concentration.
Methionine, L-[35S]- Translation Grade
Cysteine, L-[35S]Charcoal Trap for NENSureTM vial.
0695:121007
PerkinElmer, Inc.
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Boston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
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All other trademarks are the property of their respective owners.
9
1.362
The specific activity at time t (SA t) may be calculated, using the following equation,
SA t =
8
1.352
1.249
1.153
1.259
1.163