TECHNICAL DATA SHEET 35 S Caution: For Laboratory Use. A product for research purposes only. Expre35s35s, [35S]-Protein Labeling Mix Product Number: NEG072 LOT SPECIFIC INFORMATION Lot Number: ________ 01241 ___________ Specific Activity: L-[35S] Methionine: 43.5 TBq/mmol (1175.0 Ci/mmol ) L-[35S] Cysteine: 39.8 TBq/mmol (1075.0 Ci/mmol ) Concentration: Total Hydrolysate: 407.0 MBq/ml (11.0 mCi/ml ) L-[35S] Methionine: 296.0 MBq/ml (8.0 mCi/ml ) L-[35S] Cysteine: 88.8 MBq/ml (2.4 mCi/ml ) Calibration Date: ______ 18-Feb-2011 _______ CAUTION: The package size of NEG-072 refers to the total radioactivity due to L-[35S]methionine, L-[35S]cysteine and other 35S components. Pre-calibration significantly increases the actual mCi/ml and mCi/vial. Refer to the Product Specifications section for distribution of labeled components. PACKAGING: 50mM Tricine-HCl (pH 7.4) containing 10µmoles 2-mercaptoethanol per ml, in a NENSURE vial with polypropylene-v-insert, shipped in dry ice. PRODUCT SPECIFICATIONS: NEG-072, EXPRE35S35S is prepared directly from the hydrolysate of E. coli grown in the presence of carrier-free [35S] sulfate. As determined by amino acid analysis, the distribution of 35S-labeled components is routinely 73% L-[35S] methionine and 22% L-[35S] cysteine. STABILITY AND STORAGE: • • • The rate of decomposition of NEG-072, EXPRE35S35S is approximately 1% per week when stored at -80oC. Storage at higher temperatures accelerates product decomposition. Both L-[35S] methionine and L-[35S] cysteine are highly susceptible to oxidation. Precautions should be taken to minimize exposure to air. If the entire quantity will not be used at once it is recommended that the product be thawed quickly, aliquoted under N2 into samples of an appropriate size, refrozen immediately and stored at -80°C or below HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause cancer. Page 1 of 2 PRODUCT APPLICATION: NEG-072 is intended solely for use in eukaryotic or bacterial in vivo labeling studies. It is not recommended for use in cell-free mRNA-directed translations. The use of NEG-072 in the latter application provides irreproducible experimental results, with significant variation in both 35S-amino acid incorporation and background levels. SAFE HANDLING: Because this product has been stored at -80°C, it is possible that pressure may develop in the vial during the thawing process. In addition, volatile 35S-labeled decomposition products are generated at a rate of ~0.01% per week. We recommend that prior to opening, vials are first vented in a fume hood using the following procedure: • • • • Slide aside the dust cover on the cap to expose the septum. Pierce the septum with a cotton-plugged syringe needle or charcoal trap (NENTM NEX-033T), taking care that the tip does not come in contact with the product. If the product is frozen, quickly thaw at room temperature or in a 37°C water bath. Any pressure developed will vent through the syringe needle. Remove the needle and dispose of as contaminated equipment. When used for in vivo labeling experiments, we recommend that specific steps be taken to minimize incubator and water bath contamination. We suggest using a shallow tray of activated charcoal, charcoal sticks or charcoal filter units to trap 35S volatiles and reduce contamination. SPECIAL INFORMATION: Decay of 35S (physical half-life, 87.4 days): DECAY FACTORS Days BEFORE Assay Date 30 20 10 0 Days AFTER Assay Date 0 10 20 30 1 2 3 4 5 1.269 1.279 1.181 1.091 1.008 1.299 1.200 1.109 1.309 1.210 1.117 1.320 1.172 1.083 1.000 1.289 1.191 1.100 1.016 1.024 1.032 1.049 2 3 4 7 8 9 0.992 0.984 0.976 0.969 0.961 0.954 0.946 0.939 0.916 0.847 0.782 0.909 0.840 0.776 0.902 0.833 0.770 0.895 0.827 0.764 0.888 0.881 0.820 0.814 0.758 0.752 0.874 0.807 0.746 0.867 0.801 0.740 0.931 0.860 0.795 0.734 0 0 1.000 0.924 0.853 0.788 1 1.219 1.135 5 6 1.330 1.229 1.135 1.049 7 1.341 1.239 1.144 1.057 1.066 1.074 6 from the specific activity at the calibration date (SAo ) and the decay factor (f) given above. f 1/SAo (1-f)/1494 RELATED PRODUCTS: NEG-009A NEG-009C NEG-009T NEG-022T NEX-033T Methionine, L-[35S]- Premium Stabilized Grade Methionine, L-[35S]- High concentration. Methionine, L-[35S]- Translation Grade Cysteine, L-[35S]Charcoal Trap for NENSureTM vial. 0695:121007 PerkinElmer, Inc. 549 Albany Street Boston, MA 02118 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com/nenradiochemicals For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 9 1.362 The specific activity at time t (SA t) may be calculated, using the following equation, SA t = 8 1.352 1.249 1.153 1.259 1.163