Lot# APC01K109

Technical Data
Caution: For Laboratory Use. A product for research purposes only
Product Number: CR130-150
Compound:
Streptavidin conjugated to SureLight-Allophycocyanin
for kinase assays
Amount:
50.0 mg total protein
Lot Number:
APC01K109
Storage:
4C in the dark. After product has been reconstituted, DO NOT FREEZE.
Handling:
Before use, reconstitute to 25.0 ml with distilled deionized water and allow vial to sit on ice
for 20-30 minutes. Gently mix by vortexing or tapping tube before use.
Buffer:
Supplied lyophilized. Upon reconstitution with 25.0 mL distilled deionized water, the product
is in 10 mM Tris (pH 8.2), 150 mM sodium chloride, 50 mM sucrose, 0.1% BSA and 1 g/mL
pentachlorophenol.
Stability:
Upon reconstitution with water, product should be stored at 4C and be used within 8 months.
Expiration:
5/2016 when stored lyophilized.
Intended use: The Streptavidin:SureLight-Allophycocyanin conjugate can be used as an acceptor for the energy emitted by
Eu3+-chelates in the LANCE homogeneous assay format. Product is for research purposes only, the product is not intended for
any other use (such as cosmetic, agricultural, drug, food or household uses). Assay optimization should be based on the
Streptavidin concentration. One can assume that 1 Streptavidin binds two biotinylated peptides or one biotinylated protein.
Lot specific information:
(after reconstitution)
Concentration: 2.02 mg/ml total protein
SureLight-APC molarity = 9.46 M
Streptavidin molarity = 18.64 M
SureLight is a registered trademark of Columbia Biosciences Corporation
LANCE is a trademark of Perkin Elmer Corporation
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