Lot# APC01K108

Technical Data
Caution: For Laboratory Use. A product for research purposes only
Product Number: CR130-150
Compound:
Streptavidin conjugated to SureLight-Allophycocyanin
for kinase assays
Amount:
50.0 mg total protein
Lot Number:
APC01K108
Storage:
4C in the dark. After product has been reconstituted, DO NOT FREEZE.
Handling:
Before use, reconstitute to 25.0 ml with distilled deionized water and allow vial to sit on ice for 20-30
minutes. Gently mix by vortexing or tapping tube before use.
Buffer:
Supplied lyophilized. Upon reconstitution with 25.0 mL distilled deionized water, the product is in 10 mM
Tris (pH 8.2), 150 mM sodium chloride, 50 mM sucrose, 0.1% BSA and 1 g/mL pentachlorophenol.
Stability:
Upon reconstitution with water, product should be stored at 4C and be used within 8 months.
Expiration:
12/2015 when stored lyophilized.
Intended use: The Streptavidin:SureLight-Allophycocyanin conjugate can be used as an acceptor for the energy emitted by Eu 3+-chelates in
the LANCE homogeneous assay format. Product is for research purposes only, the product is not intended for any other use (such as
cosmetic, agricultural, drug, food or household uses). Assay optimization should be based on the Streptavidin concentration. One can assume
that 1 Streptavidin binds two biotinylated peptides or one biotinylated protein.
Concentration: 2.02 mg/ml total protein
SureLight-APC molarity = 10.20 M
Streptavidin molarity = 18.76 M
2.00E+06
1.60E+06
Relative Fluorescence
Lot specific information:
(after reconstitution)
1.20E+06
8.00E+05
4.00E+05
0.00E+00
600
640
680
720
760
Wavelength (nm)
SureLight is a registered trademark of Columbia Biosciences Corporation
LANCE is a trademark of Perkin Elmer Corporation
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