Technical Data Caution: For Laboratory Use. A product for research purposes only Product Number: CR130-100 Compound: Streptavidin conjugated to SureLight-Allophycocyanin for kinase assays Amount: 1.0 mg total protein Lot Number: APC1K111 Storage: 4C in the dark. After product has been reconstituted, DO NOT FREEZE. Handling: Before use, reconstitute to 1.0 ml with distilled deionized water and allow vial to sit on ice for 20-30 minutes. Gently mix by vortexing or tapping tube before use. Buffer: Supplied lyophilized. Upon reconstitution with 1.0 mL distilled deionized water, the product is in 10 mM Tris (pH 8.2), 150 mM sodium chloride, 50 mM sucrose, 0.1% BSA and 1 g/mL pentachlorophenol. Stability: Upon reconstitution with water, product should be stored at 4C and be used within 8 months. Expiration: 2/2017 when stored lyophilized. Intended use: The Streptavidin: SureLight-Allophycocyanin conjugate can be used as an acceptor for the energy emitted by Eu 3+-chelates in the LANCE homogeneous assay format. Product is for research purposes only, the product is not intended for any other use (such as cosmetic, agricultural, drug, food or household uses). Assay optimization should be based on the Streptavidin concentration. Streptavidin binds two biotinylated peptides or one biotinylated protein. 2.00E+06 Lot specific information: (after reconstitution) SureLight-APC molarity = 4.46 M Streptavidin molarity = 11.5 M Relative Fluorescence Concentration: 1.01 mg/ml total protein 1.60E+06 1.20E+06 8.00E+05 4.00E+05 0.00E+00 600 640 680 Wavelength (nm) PerkinElmer, Inc. 549 Albany Street Boston, MA 02118 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com/nenradiochemicals For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 720 760 One can assume that 1