Santa Cruz Biotechnology, Inc. SANTA CRUZ G α 16 (C-18): sc-7415 BIOTECHNOLOGY BACKGROUND Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein a, b and g polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gα subunits have been identified; these include Gs, Gi, unc and Gα 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gα i-1, Gα i-2, Gα i-3, Gα o, Gα t1, Gα t2, Gα z and Gα gust (4). Of these, the three Gα i subtypes function to open atrial potassium channels (6). Gα 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7). SOURCE G α 16 (C-18) is an affinity-purified goat polyclonal antibody mapping at the carboxy terminus of G α16 of human origin (identical to corresponding mouse sequence). PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS containing 0.1% sodium azide and 0.2% gelatin. Blocking peptide is available for competition studies (sc-7415 P) (100 µg peptide in 0.5 ml PBS with 0.1% sodium azide and 100 µg BSA). SPECIFICITY Gα 16 (C-18) reacts with Gα 16 and Gα 15 of mouse, rat and human origin by Western blotting and immunohistochemistry. STORAGE Store at 4° C, do not freeze; stable for one year from the date of shipment. RESEARCH USE For research use only, not for use in diagnostic procedures. REFERENCES 1. Conklin, B.R. and Bourne, H.R. 1993. Structural elements of Gα subunits that interact with Gβγ, receptors, and effectors. Cell 73: 631-641. 2. Cali, J.J., Balcueva, E.A., Rybalkin, I., and Robishaw, J.D. 1992. Selective tissue distribution of G protein γ subunits, including a new form of the γ subunits identified by cDNA cloning. J. Biol. Chem. 267: 24023-24027. 3. McLaughlin, S.K., McKinnon, P.J., and Margolskee, R.F. 1992. Gustducin is a taste-cell-specific G protein closely related to the transducins. Nature 357: 563-569. 4. Simon, M.I., Strathmann, M.P., and Gautam, N. 1991. Diversity of G proteins in signal transduction. Science 252: 802-808. 5. von Weizsäcker, E., Strathman, M.P., and Simon, M.I. 1992. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA. Biochem. Biophys. Res. Commun. 183: 350-356. 6. Jones, D.T., Masters, S.B., Bourne, H.R., and Reed, R.R. 1990. Biochemical characterization of three stimulatory GTP-binding proteins. J. Biol. Chem. 265: 2671-2676. 7. Amatruda, T.T. III, Steele, D.A., Slepak, V.Z., and Simon, M.I. 1991. Gα 16, a G protein alpha subunit specifically expressed in hematopoietic cells. Proc. Natl. Acad. Sci. USA 88: 5587-5591. Recommended dilution range for Western blot analysis: 1:100–1 :1000. Recommended starting dilution: 1:100. U.S. 1.800.457.3801 • 831.457.3800 • fax 831.457.3801 • Europe +800 4573 8000 • 49 (0)6221 4503 0 • fax 49 (0)6221 4503 45 • www.scbt.com July 2001 © 2002 Santa Cruz Biotechnology, Inc. All Rights Reserved.