Santa Cruz Biotechnology, Inc. SANTA CRUZ HNF-1 (H-205): sc-8986 BIOTECHNOLOGY BACKGROUND HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family (1-4). The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus (1). HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation (2,4). HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4a is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4γ is expressed in each of these tissues except liver (3). HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin (4). SOURCE HNF-1 (H-205) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 80-284 mapping within an internal region of HNF-1α of human origin. PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS containing 0.1% sodium azide and 0.2% gelatin. Also available as HNF-1 (H-205) X TransCruz antibody: sc-8986 X for gel supershift studies; supplied as 200 µg IgG in 0.1 ml PBS containing 0.1% sodium azide. STORAGE Store at 4° C, do not freeze; stable for one year from the date of shipment. SPECIFICITY HNF-1 (H-205) reacts with HNF-1α and HNF-1β of mouse, rat and human origin by Western blotting, immunoprecipitation and immunohistochemistry (including paraffin-embedded sections). HNF-1 (H-205) X TransCruz antibody is recommended for gel supershift studies. Recommended dilution range for Western blot analysis: 1:200–1:1000. Recommended starting dilution: 1:200. Molecular weight of HNF-1: 75 kDa, 67 kDa. Western blotting positive control: Hep G2 WCL, sc-2227: mouse liver extract, sc-2256; rat liver extract, sc-2395. RESEARCH USE For research use only, not for use in diagnostic procedures. REFERENCES 1. Bach, I. and Yaniv, M. 1993. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing. EMBO J. 12: 4229-4242. 2. Kaestner, K.H., Hiemisch, H., Luckow, B., and Schutz, G. 1994. The HNF-3 gene family of transcription factors in mice:gene structure, cDNA sequence, and mRNA distribution. Genomics 20: 377-385. 3. Drewes, T., Senkel, S., Holewa, B., and Ryffel, G.U. 1996. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes. Mol. Cell Biol. 16: 925-931. 4. Samadani, U. and Costa, R.H. 1996. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression. Mol. Cell Biol. 16: 6273-6284. U.S. 1.800.457.3801 • 831.457.3800 • fax 831.457.3801 • Europe +800 4573 8000 • 49 (0)6221 4503 0 • fax 49 (0)6221 4503 45 • www.scbt.com April 2000 © 2002 Santa Cruz Biotechnology, Inc. All Rights Reserved.