photon detection unit c9692 series

PHOTON
DETECTION
LOW-LIGHT-LEVEL UNIT
DETECTION
C9692 SERIES
C9692-11
C9692-12
C9692-13
Excitation
light source unit
Optical fiber panel
Sample holder unit
C9692-14
C9692-15
Sample holder unit
C9692-16
Syringe
(sold separately)
Dispenser unit
Sample holder unit
Optical block panel
Microtube unit
OVERVIEW
The C9692 series Photon Detection Units are photon counting units designed to make low-light measurements at single photon levels without a time-consuming measurement
setup. All users need to do is prepare the sample the users
want to measure and a personal computer (PC). Six models
are available so the users can select one that best meets
your application.
When combined with optional modular units the C9692 is
ideal for various types of measurement. The USB interface
allows simple plug & play setup when connecting the C9692
to a PC.
APPLICATIONS
●Bioluminescence, chemiluminescence
●Food oxidation, antioxidant activity luminescence
●Activated cell luminescence
●UV-excited (UV LED) delayed fluorescence (C9692-13)
●Other low-light-level measurements
FEATURES
●Photon counting with high S/N ratio
Low noise: 50 s-1 (Typ.at 25 °C)
●Computer control
Built-in USB interface
●CE marking compliance
●Optical fiber (FC type) compatible (C9692-11)
●Interlock function (C9692-12/-13/-14/-15)
Automatically closes optical shutter to prevent excessive light from entering PMT if sample compartment is accidentally opened during measurement.
●Built-in UV LED excitation light source (C9692-13)
Light source wavelength: 375 nm
Output power: 10 mW/cm2
Irradiation time: 0.1 to 3600 seconds
●Reagent dispensing (C9692-14/-15)
Dispenses two types of reagents using syringe (C9692-14),
pipette (C9692-15) as a dispenser
APPLICATIONS
Application 1 Evaluating compost maturity
Compost
Hydrothermal Extract liquid
extraction
from compost
Measure
5000
4000
IMMATURE COMPOST
3000
MEDIUM MATURE COMPOST
2000
FULLY MATURE COMPOST
100
0
0
100
200
C9692-12
Oxygen
injection
Filtration
6000
LUMINESCENCE INTENSITY (counts/10s)
Organic farming is the focus of much recent attention, and is a field
where the market value of good quality compost is on the rise. To ensure
that good quality compost can be quickly supplied when needed, photon
detection units are used in research to develop techniques for
determining compost maturity during the compost purification process.
Focusing on the fact that good quality compost is oxidized excrement,
researchers added oxygen to liquid extracted from compost during the
fermentation process to accelerate oxidation of residual organic matter
and then rated the maturation from the intensity of low-level luminescence
emitted during the oxidation process. Good quality compost has less
unoxidized material and so emits low-level luminescence.
300
400
500
600
TIME (s)
Data courtesy of:
Nobuya Katayama, Shizuoka Prefectural
Animal Husbandry Experiment Station
Mayuko Iwai, Graduate School for Creation of
New Photonics Industries
Luminescence
Application 2 Diagnosing fungus-infected plants
C9692-12
Tree bark
Results from measuring luminescence intensity
on fruited body impossible to observe visually
over elapsed time
400000
LUMINESCENCE INTENSITY (counts/s)
Photon detection units are utilized in research to develop techniques for
detecting the low level luminescence emitted from honey fungus which is
a type of mushroom that acts as a parasite and eats into road-side trees.
These techniques will serve as tests to diagnose whether a tree is
infected with fungal filaments (hypha).
Samples taken from the bark of the suspect tree are measured using a
photon detection unit.
The material within the tree bark being eaten away by the fungus
undergoes a temperature change due to fungal action so that the
luminescence intensity rises with the passage of time. Utilizing these
changes in low-level luminescence intensity
reveals whether there is fungal infection or not.
300000
200000
100000
0
Luminescence
0
30
60
90
TIME (min)
Upper: Honey fungus (fruited body: luminescence cannot be observed visually)
Left: Image observed on fungus culture under bright conditions
Right: Image observed on fungus culture under dark conditions (luminescence can be observed visually)
Data courtesy of:
Masaru Hiroi, Koriyama Women’s University & College
Application 3 Oxidization on one polished rice grain
(Approximate values)
90
PHOTON DETECTION UNIT C9692
80
PHOTOMULTIPLIER
TUBE
MODULE
SHUTTER
LOW LEVEL LIGHT
FLUORESCENCE
EXCITATION
LIGHT UNIT
A9860
UV LED
SAMPLE
FLUORESCENCE INTENSITY
The delayed fluorescence intensity from
the surface of a single polished rice grain
irradiated with UV light for 10 seconds is
measured using a photon detection unit
that contains an excitation light source
and is operated with dedicated software.
This measurement yielded a specific
value for progressive oxidation after rice
milling with the
C9692-13
passage of time.
70
60
50
AFTER RICE MILLING
40
6 MONTHS AFTER RICE MILLING
30
20
10
SAMPLE
HOLDER
UNIT
A9861
0
0
0.5
1
1.5
TIME (s)
2
2.5
3
12
16
ATP LUMINESCENCE LEVEL
KOJI ASPERGILLUS MASS
10
12
8
6
8
4
4
2
0
0
16
8
0
32
24
KOJI ASPERGILLUS BODY MASS (mg/g OF KOJI)
Photon detection units were used to measure the luminescence level
in the APT method and changes in koji aspergillus body mass of rice
koji cultivated based on the standard koji making test.
Comparing the luminescence level in the APT method with the koji aspergillus body mass, they show similar changes up to 32 consecutive
hours of rice koji cultivation. Changes in the rice body mass and enzymatic activity (alpha-amylase) are major quality indicators of koji aspergillus and mainly end during the logarithmic growth phase, so how
both methods related was compared in a range of the culture time
from 12 to 32 hours,
which is a transition to
C9692-15
the stationary phase.
Although the number of
data was small, the results clearly showed a
high correlation.
LUMINESCENCE LEVEL (×105 counts/10s)
Application 4 Luminescence level in ATP method and changes in koji aspergillus body mass during cultivation of rice koji
CULTURE TIME (h)
Data courtesy of:
Prof. Takahiro Saito, Faculty of Agriculture, Department of
Environmental Engineering, Utsunomiya University
Photon detection units are used in research to find techniques using
chemiluminescence for quickly and easily measuring the degree of
purity in food.
About 3 leaves each taken from the outermost layer of several pieces
of Boston lettuce were thoroughly crushed and diluted about 10 times
with distilled sterile water for use as the sample fluid concentrate and
the luminescence intensity measured by the ATP method using the
photon detection unit.
The number of viable
C9692-15
bacteria cultured by the
official analytical method
was then found and the
correlation with the luminescence level found.
VIABLE BACTERIA COUNT (Log of CFU/g)
Application 5 Viable bacteria count versus luminescence level in ATP method on fresh produce
7
6
5
4
3
y = 1.33 × -1.52
R2 = 0.80
2
1
0
0
1
2
4
3
6
5
7
LUMINESCENCE LEVEL (Log of counts/10s)
Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University
Application 6 Evaluation of refined sake deterioration
Evaluation by oxidation reaction measurement system (lower graph)
The degradation (or oxidation) occurring in refined sake was evaluated using a mixture of 75 microliters of hypochlorous natrium to 3 milliliters of refined sake.
In both methods the luminescence intensity (degradation level) rose in proportion to the number of days in storage.
Left standing
in 30 °C
environment
C9692-14
LUMINESCENCE INTENSITY (count/s)
Evaluation by XYZ measurement system (upper graph)
The degradation (or oxidation) occurring in refined sake was evaluated by
ranking refined sake as reactive oxygen species (X) and using a mixture of
anti-oxidation species (Y) and receptor species (Z).
Evaluation by XYZ measurement system
100
60 days
30 days
0 days
90
80
70
60
50
40
30
20
10
0
0
50
100
150
200
TIME (s)
Evaluation by oxidation reaction measurement system
LUMINESCENCE INTENSITY (count/s)
Refined sake (rice wine) oxidizes or in other words degrades after the container or bottle is opened. This oxidation was evaluated with photon detection units utilizing the following two methods.
15000
60 days
30 days
0 days
12000
9000
6000
3000
0
0
5
10
15
20
25
30
TIME (s)
Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University
SPECIFICATIONS
C9692 SERIES COMMON SPECIFICATIONS
Parameter
Detection Method
Spectral Response Range
Photocathode Size
Maximum Count Rate
Counter Gate Time
Dark Count (Typ. at 25 °C)
Counter Capacity
Trigger Signal Input Mode
Trigger Signal Level
Trigger Section
Trigger Signal Pulse Width
Input Voltage (DC)
Input Voltage (AC) to Supplied AC Adapter
Temperature
Operating
Humidity
Temperature
Storage
Humidity
OS
Interface
Description / Value
Photon counting method
185 to 650
16 × 18
3 × 106
0.001 to 10 (1, 2, 5 Steps)
50
32 bits/gate
External trigger, software trigger
TTL negative logic
100 ns or more
+7 (supplied from AC adapter)
100 V to 240 V (auto switchable), single phase 50 Hz/60 Hz
+5 to +40
Below 80 (no condensation)
0 to +50
Below 85 (no condensation)
Windows® XP-Pro, Vista Business (32 bit), 7 Pro (32 bit)
USB
Unit
—
nm
mm
s-1
s
s-1
—
—
—
—
V
—
°C
%
°C
%
—
—
Description / Value
FC type (HRFC-R1/Hirose)
21.0 (from fiber end)
Approx. 0.7
Unit
—
mm
kg
Description / Value
50 × 50 × 15
32.5 (from bottom of sample compartment)
Approx. 1.1
Unit
mm
mm
kg
C9692-11 (Optical Fiber Panel Type)
Parameter
Optical Fiber Adapter
Distance to Photocathode
Weight
C9692-12 (Sample Holder Unit Type)
Parameter
Effective Size of Sample Compartment (W × D × H)
Distance to Photocathode
Weight
C9692-13 (Sample Holder Unit + Excitation Light Source Unit Type)
Parameter
Wavelength · Output Power
Excitation Light
Irradiation Time
Source (UV LED)
Irradiation Area
Effective Size of Sample Compartment (W × D × H)
Distance to Photocathode
Weight
Description / Value
375 nm ·10 mW/cm2
0.1 to 3600
10 (center of sample compartment)
50 × 50 × 15
49.5 (from bottom of sample compartment)
Approx. 1.2
Unit
—
s
mm
mm
mm
kg
C9692-14 (Sample Holder Unit + Dispenser Unit Type)
Parameter
Suitable Syringe Capacity
Needle Size
Recommended Gas Flow Tube
Distance to Photocathode
Weight
Description / Value
5 (Terumo syringe)
Outer diameter: 1 mm, inner diameter: 0.6 mm
Outer diameter: 6 mm (Black tube is recommended for light shielding)
49.5
Approx. 1.2
Unit
ml
—
—
mm
kg
Description / Value
500
23.0
Approx. 1.4
Unit
µl
mm
kg
Description / Value
V-groove type
23.3
Approx. 0.7
Unit
—
mm
kg
C9692-15 (Microtube Unit Type)
Parameter
Suitable Microtube
Distance to Photocathode (to microtube surface)
Weight
C9692-16 (Optical Block Panel Type)
Parameter
Suitable Optical Block
Distance to Photocathode
Weight
ACCESSORIES (Supplied)
●CD-ROM (control software)
●USB cable (1.5 m)
●AC adapter
●Cable for external trigger (1.5 m)
●1-rack (96 pcs) of pipette tips (C9692-15)
CONTROL SOFTWARE FUNCTIONS
●Time-resolved measurement
Resolves measurement time per unit time (1 ms or more) allowing measurement of various light emission patterns.
●Optical shutter control
Opens or closes optical shutter for excess light protection and dark current pulse measurement.
●Data display during measurement
Continuously transfers measurement data to PC for data monitoring.
●Measurement data save
Saves measurement data in Excel format to make data analysis easier.
OPTIONAL MODULAR UNITS (available at additional cost)
Optical Fiber Panel A9859
Excitation Light Source Unit A9860
Sample Holder Unit A9861
Disperser Unit A10490
Microtube Unit A11044
Pipette Tips A11045
(A11045: 10-rack set)
Optical Block Panel A9859-01
SETUP DIAGRAMS
●C9692-11
●C9692-12
Major applications: Various measurements using optical fiber (FC type)
PHOTON DETECTION UNIT C9692
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
Major applications: Bioluminescence, chemiluminescence
PHOTON DETECTION UNIT C9692
PHOTOMULTIPLIER
TUBE
MODULE
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
PHOTOMULTIPLIER
TUBE
MODULE
USB
SHUTTER
SHUTTER
LOW LEVEL
LIGHT
USB
LOW LEVEL
LIGHT
PC
OPTICAL FIBER PANEL
A9859
PC
SAMPLE HOLDER UNIT
A9861
SAMPLE
OPTICAL FIBER (FC TYPE)
TPMOC0190EA
●C9692-13
TPMOC0191EA
●C9692-14
Major applications: Fluorescence, delayed fluorescence, phosphorescence
PHOTON DETECTION UNIT C9692
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
Major applications: Chemiluminescence
PHOTON DETECTION UNIT C9692
PHOTOMULTIPLIER
TUBE
MODULE
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
PHOTOMULTIPLIER
TUBE
MODULE
USB
SHUTTER
SHUTTER
LOW LEVEL LIGHT
FLUORESCENCE
USB
LOW LEVEL
LIGHT
PC
PC
EXCITATION LIGHT UNIT
A9860
DISPENSER UNIT
A10490
SAMPLE HOLDER UNIT
A9861
SAMPLE HOLDER UNIT
A9861
UV LED
SAMPLE
SAMPLE
TPMOC0188EA
●C9692-15
TPMOC0205EA
●C9692-16
Major applications: Chemiluminescence
Major applications: Measurements with optical blocks
PIPETTE TIP A11045
PHOTON DETECTION UNIT C9692
LOW LEVEL LIGHT
MICROTUBE
UNIT
A11044
SHUTTER
MICROTUBE
PHOTOMULTIPLIER
TUBE
MODULE
PHOTON DETECTION UNIT C9692
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
DC +7 V IN
AC ADAPTER
(SUPPLIED)
AC100 V to 240 V
PHOTOMULTIPLIER
TUBE
MODULE
USB
SHUTTER
PC
USB
LOW LEVEL
LIGHT
PC
OPTICAL BLOCK PANEL
A9859-01
OPTICAL BLOCK
TPMZC0014EA
TPMZC0015EA
DIMENSIONAL OUTLINES (Unit: mm)
●C9692-11
FRONT VIEW
PHOTON DETECTION UNIT C9692
ON
OFF
PANEL
POWER
ERROR
PMT POWER
EXT. TRIG
100
SIDE VIEW
DC IN
USB
SIDE VIEW
101.5
100
8.5
42
OPTICAL FIBER ADAPTER
FC TYPE: HRFC-R1 (HIROSE)
●C9692-12
TOP VIEW
FRONT VIEW
TPMOA0033EA
SIDE VIEW
PHOTON DETECTION UNIT C9692
EXT. TRIG
PMT POWER
124.5
100
USB
ERROR
POWER
OFF
DC IN
PANEL
15
8
30
ON
100
17
50
SAMPLE COMPARTMENT
78
●C9692-13
TOP VIEW
TPMOA0034EA
FRONT VIEW
SIDE VIEW
PHOTON DETECTION UNIT C9692
EXT. TRIG
PMT POWER
142
100
USB
ERROR
POWER
OFF
18
DC IN
30
100
UV LED
15
ON
8
PANEL
17
50
SAMPLE COMPARTMENT
78
100
●C9692-14
TPMOA0035EA
FRONT VIEW
TOP VIEW
SIDE VIEW
PHOTON DETECTION UNIT C9692
EXT. TRIG
ID SW
PMT POWER
SYRINGE
INSERTION
PORT
142
POWER
GAS PORT
OFF
ON
100
50
78
100
15
18
DC IN
8
PANEL
30
100
USB
ERROR
17
100±0.5
SAMPLE COMPARTMENT
TPMOA0045EA
DIMENSIONAL OUTLINES (Unit: mm)
●C9692-15
PHOTON DETECTION UNIT C9692
PIPETTE TIP INSERTION PORT
SAMPLE EXCHANGE DOOR
ON
126.5 ± 2.0
DC IN
PANEL
OFF
POWER
ERROR
USB
PMT POWER
ID SW
●C9692-16
12
160 ± 1
SIDE VIEW
PHOTON DETECTION UNIT C9692
DC IN
ON
OFF
PANEL
POWER
ERROR
PMT POWER
EXT. TRIG
TPMOA0003EA
FRONT VIEW
USB
ID SW
SIDE VIEW
100
EXT. TRIG
72 ± 1
100 ± 1
96.5
100
10.8
42
TPMZA0013EA
●Sample compartment tray (same for C9692-12, -13, -14)
TOP VIEW
4-R3
17
96
69.8
56
50
78
56
EFFECTIVE SIZE OF
SAMPLE COMPARTMENT
(W50 × D50 × H15)
TPMOA0034EA / TPMOA0035EA / TPMOA0045EA
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Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are
subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2012 Hamamatsu Photonics K.K.
HAMAMATSU PHOTONICS K.K.
www.hamamatsu.com
HAMAMATSU PHOTONICS K.K., Electron Tube Division
314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205
U.S.A.: Hamamatsu Corporation: 360 Foothill Road, P. O. Box 6910, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected]
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TPMZ1010E01
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JAN. 2012 IP