PHOTON DETECTION LOW-LIGHT-LEVEL UNIT DETECTION C9692 SERIES C9692-11 C9692-12 C9692-13 Excitation light source unit Optical fiber panel Sample holder unit C9692-14 C9692-15 Sample holder unit C9692-16 Syringe (sold separately) Dispenser unit Sample holder unit Optical block panel Microtube unit OVERVIEW The C9692 series Photon Detection Units are photon counting units designed to make low-light measurements at single photon levels without a time-consuming measurement setup. All users need to do is prepare the sample the users want to measure and a personal computer (PC). Six models are available so the users can select one that best meets your application. When combined with optional modular units the C9692 is ideal for various types of measurement. The USB interface allows simple plug & play setup when connecting the C9692 to a PC. APPLICATIONS ●Bioluminescence, chemiluminescence ●Food oxidation, antioxidant activity luminescence ●Activated cell luminescence ●UV-excited (UV LED) delayed fluorescence (C9692-13) ●Other low-light-level measurements FEATURES ●Photon counting with high S/N ratio Low noise: 50 s-1 (Typ.at 25 °C) ●Computer control Built-in USB interface ●CE marking compliance ●Optical fiber (FC type) compatible (C9692-11) ●Interlock function (C9692-12/-13/-14/-15) Automatically closes optical shutter to prevent excessive light from entering PMT if sample compartment is accidentally opened during measurement. ●Built-in UV LED excitation light source (C9692-13) Light source wavelength: 375 nm Output power: 10 mW/cm2 Irradiation time: 0.1 to 3600 seconds ●Reagent dispensing (C9692-14/-15) Dispenses two types of reagents using syringe (C9692-14), pipette (C9692-15) as a dispenser APPLICATIONS Application 1 Evaluating compost maturity Compost Hydrothermal Extract liquid extraction from compost Measure 5000 4000 IMMATURE COMPOST 3000 MEDIUM MATURE COMPOST 2000 FULLY MATURE COMPOST 100 0 0 100 200 C9692-12 Oxygen injection Filtration 6000 LUMINESCENCE INTENSITY (counts/10s) Organic farming is the focus of much recent attention, and is a field where the market value of good quality compost is on the rise. To ensure that good quality compost can be quickly supplied when needed, photon detection units are used in research to develop techniques for determining compost maturity during the compost purification process. Focusing on the fact that good quality compost is oxidized excrement, researchers added oxygen to liquid extracted from compost during the fermentation process to accelerate oxidation of residual organic matter and then rated the maturation from the intensity of low-level luminescence emitted during the oxidation process. Good quality compost has less unoxidized material and so emits low-level luminescence. 300 400 500 600 TIME (s) Data courtesy of: Nobuya Katayama, Shizuoka Prefectural Animal Husbandry Experiment Station Mayuko Iwai, Graduate School for Creation of New Photonics Industries Luminescence Application 2 Diagnosing fungus-infected plants C9692-12 Tree bark Results from measuring luminescence intensity on fruited body impossible to observe visually over elapsed time 400000 LUMINESCENCE INTENSITY (counts/s) Photon detection units are utilized in research to develop techniques for detecting the low level luminescence emitted from honey fungus which is a type of mushroom that acts as a parasite and eats into road-side trees. These techniques will serve as tests to diagnose whether a tree is infected with fungal filaments (hypha). Samples taken from the bark of the suspect tree are measured using a photon detection unit. The material within the tree bark being eaten away by the fungus undergoes a temperature change due to fungal action so that the luminescence intensity rises with the passage of time. Utilizing these changes in low-level luminescence intensity reveals whether there is fungal infection or not. 300000 200000 100000 0 Luminescence 0 30 60 90 TIME (min) Upper: Honey fungus (fruited body: luminescence cannot be observed visually) Left: Image observed on fungus culture under bright conditions Right: Image observed on fungus culture under dark conditions (luminescence can be observed visually) Data courtesy of: Masaru Hiroi, Koriyama Women’s University & College Application 3 Oxidization on one polished rice grain (Approximate values) 90 PHOTON DETECTION UNIT C9692 80 PHOTOMULTIPLIER TUBE MODULE SHUTTER LOW LEVEL LIGHT FLUORESCENCE EXCITATION LIGHT UNIT A9860 UV LED SAMPLE FLUORESCENCE INTENSITY The delayed fluorescence intensity from the surface of a single polished rice grain irradiated with UV light for 10 seconds is measured using a photon detection unit that contains an excitation light source and is operated with dedicated software. This measurement yielded a specific value for progressive oxidation after rice milling with the C9692-13 passage of time. 70 60 50 AFTER RICE MILLING 40 6 MONTHS AFTER RICE MILLING 30 20 10 SAMPLE HOLDER UNIT A9861 0 0 0.5 1 1.5 TIME (s) 2 2.5 3 12 16 ATP LUMINESCENCE LEVEL KOJI ASPERGILLUS MASS 10 12 8 6 8 4 4 2 0 0 16 8 0 32 24 KOJI ASPERGILLUS BODY MASS (mg/g OF KOJI) Photon detection units were used to measure the luminescence level in the APT method and changes in koji aspergillus body mass of rice koji cultivated based on the standard koji making test. Comparing the luminescence level in the APT method with the koji aspergillus body mass, they show similar changes up to 32 consecutive hours of rice koji cultivation. Changes in the rice body mass and enzymatic activity (alpha-amylase) are major quality indicators of koji aspergillus and mainly end during the logarithmic growth phase, so how both methods related was compared in a range of the culture time from 12 to 32 hours, which is a transition to C9692-15 the stationary phase. Although the number of data was small, the results clearly showed a high correlation. LUMINESCENCE LEVEL (×105 counts/10s) Application 4 Luminescence level in ATP method and changes in koji aspergillus body mass during cultivation of rice koji CULTURE TIME (h) Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University Photon detection units are used in research to find techniques using chemiluminescence for quickly and easily measuring the degree of purity in food. About 3 leaves each taken from the outermost layer of several pieces of Boston lettuce were thoroughly crushed and diluted about 10 times with distilled sterile water for use as the sample fluid concentrate and the luminescence intensity measured by the ATP method using the photon detection unit. The number of viable C9692-15 bacteria cultured by the official analytical method was then found and the correlation with the luminescence level found. VIABLE BACTERIA COUNT (Log of CFU/g) Application 5 Viable bacteria count versus luminescence level in ATP method on fresh produce 7 6 5 4 3 y = 1.33 × -1.52 R2 = 0.80 2 1 0 0 1 2 4 3 6 5 7 LUMINESCENCE LEVEL (Log of counts/10s) Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University Application 6 Evaluation of refined sake deterioration Evaluation by oxidation reaction measurement system (lower graph) The degradation (or oxidation) occurring in refined sake was evaluated using a mixture of 75 microliters of hypochlorous natrium to 3 milliliters of refined sake. In both methods the luminescence intensity (degradation level) rose in proportion to the number of days in storage. Left standing in 30 °C environment C9692-14 LUMINESCENCE INTENSITY (count/s) Evaluation by XYZ measurement system (upper graph) The degradation (or oxidation) occurring in refined sake was evaluated by ranking refined sake as reactive oxygen species (X) and using a mixture of anti-oxidation species (Y) and receptor species (Z). Evaluation by XYZ measurement system 100 60 days 30 days 0 days 90 80 70 60 50 40 30 20 10 0 0 50 100 150 200 TIME (s) Evaluation by oxidation reaction measurement system LUMINESCENCE INTENSITY (count/s) Refined sake (rice wine) oxidizes or in other words degrades after the container or bottle is opened. This oxidation was evaluated with photon detection units utilizing the following two methods. 15000 60 days 30 days 0 days 12000 9000 6000 3000 0 0 5 10 15 20 25 30 TIME (s) Data courtesy of: Prof. Takahiro Saito, Faculty of Agriculture, Department of Environmental Engineering, Utsunomiya University SPECIFICATIONS C9692 SERIES COMMON SPECIFICATIONS Parameter Detection Method Spectral Response Range Photocathode Size Maximum Count Rate Counter Gate Time Dark Count (Typ. at 25 °C) Counter Capacity Trigger Signal Input Mode Trigger Signal Level Trigger Section Trigger Signal Pulse Width Input Voltage (DC) Input Voltage (AC) to Supplied AC Adapter Temperature Operating Humidity Temperature Storage Humidity OS Interface Description / Value Photon counting method 185 to 650 16 × 18 3 × 106 0.001 to 10 (1, 2, 5 Steps) 50 32 bits/gate External trigger, software trigger TTL negative logic 100 ns or more +7 (supplied from AC adapter) 100 V to 240 V (auto switchable), single phase 50 Hz/60 Hz +5 to +40 Below 80 (no condensation) 0 to +50 Below 85 (no condensation) Windows® XP-Pro, Vista Business (32 bit), 7 Pro (32 bit) USB Unit — nm mm s-1 s s-1 — — — — V — °C % °C % — — Description / Value FC type (HRFC-R1/Hirose) 21.0 (from fiber end) Approx. 0.7 Unit — mm kg Description / Value 50 × 50 × 15 32.5 (from bottom of sample compartment) Approx. 1.1 Unit mm mm kg C9692-11 (Optical Fiber Panel Type) Parameter Optical Fiber Adapter Distance to Photocathode Weight C9692-12 (Sample Holder Unit Type) Parameter Effective Size of Sample Compartment (W × D × H) Distance to Photocathode Weight C9692-13 (Sample Holder Unit + Excitation Light Source Unit Type) Parameter Wavelength · Output Power Excitation Light Irradiation Time Source (UV LED) Irradiation Area Effective Size of Sample Compartment (W × D × H) Distance to Photocathode Weight Description / Value 375 nm ·10 mW/cm2 0.1 to 3600 10 (center of sample compartment) 50 × 50 × 15 49.5 (from bottom of sample compartment) Approx. 1.2 Unit — s mm mm mm kg C9692-14 (Sample Holder Unit + Dispenser Unit Type) Parameter Suitable Syringe Capacity Needle Size Recommended Gas Flow Tube Distance to Photocathode Weight Description / Value 5 (Terumo syringe) Outer diameter: 1 mm, inner diameter: 0.6 mm Outer diameter: 6 mm (Black tube is recommended for light shielding) 49.5 Approx. 1.2 Unit ml — — mm kg Description / Value 500 23.0 Approx. 1.4 Unit µl mm kg Description / Value V-groove type 23.3 Approx. 0.7 Unit — mm kg C9692-15 (Microtube Unit Type) Parameter Suitable Microtube Distance to Photocathode (to microtube surface) Weight C9692-16 (Optical Block Panel Type) Parameter Suitable Optical Block Distance to Photocathode Weight ACCESSORIES (Supplied) ●CD-ROM (control software) ●USB cable (1.5 m) ●AC adapter ●Cable for external trigger (1.5 m) ●1-rack (96 pcs) of pipette tips (C9692-15) CONTROL SOFTWARE FUNCTIONS ●Time-resolved measurement Resolves measurement time per unit time (1 ms or more) allowing measurement of various light emission patterns. ●Optical shutter control Opens or closes optical shutter for excess light protection and dark current pulse measurement. ●Data display during measurement Continuously transfers measurement data to PC for data monitoring. ●Measurement data save Saves measurement data in Excel format to make data analysis easier. OPTIONAL MODULAR UNITS (available at additional cost) Optical Fiber Panel A9859 Excitation Light Source Unit A9860 Sample Holder Unit A9861 Disperser Unit A10490 Microtube Unit A11044 Pipette Tips A11045 (A11045: 10-rack set) Optical Block Panel A9859-01 SETUP DIAGRAMS ●C9692-11 ●C9692-12 Major applications: Various measurements using optical fiber (FC type) PHOTON DETECTION UNIT C9692 DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V Major applications: Bioluminescence, chemiluminescence PHOTON DETECTION UNIT C9692 PHOTOMULTIPLIER TUBE MODULE DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V PHOTOMULTIPLIER TUBE MODULE USB SHUTTER SHUTTER LOW LEVEL LIGHT USB LOW LEVEL LIGHT PC OPTICAL FIBER PANEL A9859 PC SAMPLE HOLDER UNIT A9861 SAMPLE OPTICAL FIBER (FC TYPE) TPMOC0190EA ●C9692-13 TPMOC0191EA ●C9692-14 Major applications: Fluorescence, delayed fluorescence, phosphorescence PHOTON DETECTION UNIT C9692 DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V Major applications: Chemiluminescence PHOTON DETECTION UNIT C9692 PHOTOMULTIPLIER TUBE MODULE DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V PHOTOMULTIPLIER TUBE MODULE USB SHUTTER SHUTTER LOW LEVEL LIGHT FLUORESCENCE USB LOW LEVEL LIGHT PC PC EXCITATION LIGHT UNIT A9860 DISPENSER UNIT A10490 SAMPLE HOLDER UNIT A9861 SAMPLE HOLDER UNIT A9861 UV LED SAMPLE SAMPLE TPMOC0188EA ●C9692-15 TPMOC0205EA ●C9692-16 Major applications: Chemiluminescence Major applications: Measurements with optical blocks PIPETTE TIP A11045 PHOTON DETECTION UNIT C9692 LOW LEVEL LIGHT MICROTUBE UNIT A11044 SHUTTER MICROTUBE PHOTOMULTIPLIER TUBE MODULE PHOTON DETECTION UNIT C9692 DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V DC +7 V IN AC ADAPTER (SUPPLIED) AC100 V to 240 V PHOTOMULTIPLIER TUBE MODULE USB SHUTTER PC USB LOW LEVEL LIGHT PC OPTICAL BLOCK PANEL A9859-01 OPTICAL BLOCK TPMZC0014EA TPMZC0015EA DIMENSIONAL OUTLINES (Unit: mm) ●C9692-11 FRONT VIEW PHOTON DETECTION UNIT C9692 ON OFF PANEL POWER ERROR PMT POWER EXT. TRIG 100 SIDE VIEW DC IN USB SIDE VIEW 101.5 100 8.5 42 OPTICAL FIBER ADAPTER FC TYPE: HRFC-R1 (HIROSE) ●C9692-12 TOP VIEW FRONT VIEW TPMOA0033EA SIDE VIEW PHOTON DETECTION UNIT C9692 EXT. TRIG PMT POWER 124.5 100 USB ERROR POWER OFF DC IN PANEL 15 8 30 ON 100 17 50 SAMPLE COMPARTMENT 78 ●C9692-13 TOP VIEW TPMOA0034EA FRONT VIEW SIDE VIEW PHOTON DETECTION UNIT C9692 EXT. TRIG PMT POWER 142 100 USB ERROR POWER OFF 18 DC IN 30 100 UV LED 15 ON 8 PANEL 17 50 SAMPLE COMPARTMENT 78 100 ●C9692-14 TPMOA0035EA FRONT VIEW TOP VIEW SIDE VIEW PHOTON DETECTION UNIT C9692 EXT. TRIG ID SW PMT POWER SYRINGE INSERTION PORT 142 POWER GAS PORT OFF ON 100 50 78 100 15 18 DC IN 8 PANEL 30 100 USB ERROR 17 100±0.5 SAMPLE COMPARTMENT TPMOA0045EA DIMENSIONAL OUTLINES (Unit: mm) ●C9692-15 PHOTON DETECTION UNIT C9692 PIPETTE TIP INSERTION PORT SAMPLE EXCHANGE DOOR ON 126.5 ± 2.0 DC IN PANEL OFF POWER ERROR USB PMT POWER ID SW ●C9692-16 12 160 ± 1 SIDE VIEW PHOTON DETECTION UNIT C9692 DC IN ON OFF PANEL POWER ERROR PMT POWER EXT. TRIG TPMOA0003EA FRONT VIEW USB ID SW SIDE VIEW 100 EXT. TRIG 72 ± 1 100 ± 1 96.5 100 10.8 42 TPMZA0013EA ●Sample compartment tray (same for C9692-12, -13, -14) TOP VIEW 4-R3 17 96 69.8 56 50 78 56 EFFECTIVE SIZE OF SAMPLE COMPARTMENT (W50 × D50 × H15) TPMOA0034EA / TPMOA0035EA / TPMOA0045EA Windows® is a registered trademark of Microsoft Corporation in the United States and/or other countries. Other product names, software names and company names mentioned herein may be the trademarks of their respective owners. Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2012 Hamamatsu Photonics K.K. HAMAMATSU PHOTONICS K.K. www.hamamatsu.com HAMAMATSU PHOTONICS K.K., Electron Tube Division 314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205 U.S.A.: Hamamatsu Corporation: 360 Foothill Road, P. O. Box 6910, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road Welwyn Garden City Hertfordshire AL7 1BW, United Kingdom, Telephone: 44-(0)1707-294888, Fax: 44(0)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Smidesvägen 12, SE-171-41 SOLNA, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] TPMZ1010E01 Italy: Hamamatsu Photonics Italia: S.R.L.: Strada della Moia, 1/E, 20020 Arese, (Milano), Italy, Telephone: (39)02-935 81 733, Fax: (39)02-935 81 741 E-mail: [email protected] China: HAMAMATSU PHOTONICS (CHINA) Co., Ltd.: 1201 Tower B, Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected] JAN. 2012 IP